Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection
| Autor(a) principal: | |
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| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://doi.org/10.1186/s13071-015-1259-6 |
Resumo: | PMID: 26684322 WOS:000367080800004 |
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Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infectionParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingPMID: 26684322 WOS:000367080800004Background: Visceral leishmaniasis is a severe and potentially fatal disease caused by protozoa of the genus Leishmania, transmitted by phlebotomine sandflies. In Europe and the Mediterranean region, L. infantum is the commonest agent of visceral leishmaniasis, causing a wide spectrum of clinical manifestations, including asymptomatic carriage, cutaneous lesions and severe visceral disease. Visceral leishmaniasis is more frequent in immunocompromised individuals and data obtained in experimental models of infection have highlighted the importance of the host immune response, namely the efficient activation of host's macrophages, in determining infection outcome. Conversely, few studies have addressed a possible contribution of parasite variability to this outcome. Methods: In this study, we compared three isolates of L. infantum regarding their capacity to grow in the organs of mice, the way they activate the host's macrophages and other components of the immune response and also their capacity to cope with host's antimicrobial mechanisms, namely reactive oxygen and nitrogen species. Results: We found that the three parasite strains significantly differed regarding the degree to which they induced nitric oxide synthase (NOS2) and arginase expression in infected macrophages and the pattern of cytokine production they induced in the host, resulting in different degrees of inflammatory response in infected livers. Additionally, the three strains also significantly differed in their in vitro susceptibility to reactive oxygen and nitrogen species. This variability was reflected in the capacity of each strain to persist and proliferate in the organs of wild-type as well as NOS2- and phagocyte oxidase- deficient mice. Conclusions: The results obtained in this study show that parasite strain variability is an important determinant of disease outcome in L. infantum visceral leishmaniasis, with relevant implications for studies on host-pathogen interaction and also for leishmanicidal drug development.Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)Instituto de Higiene e Medicina Tropical (IHMT)RUNMarques, FilipeVale-Costa, SílviaCruz, TâniaMarques, Joana MoreiraSilva, TâniaNeves, João VilaresCortes, SofiaFernandes, AnaRocha, EduardoAppelberg, RuiRodrigues, PedroTomás, Ana M.Gomes, Maria Salomé2018-05-11T22:02:55Z2015-12-182015-12-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1186/s13071-015-1259-6engPURE: 1729028http://www.scopus.com/inward/record.url?scp=84950313576&partnerID=8YFLogxKhttps://doi.org/10.1186/s13071-015-1259-6info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:03Zoai:run.unl.pt:10362/36589Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:34.419693Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection |
| title |
Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection |
| spellingShingle |
Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection Marques, Filipe Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
| title_short |
Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection |
| title_full |
Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection |
| title_fullStr |
Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection |
| title_full_unstemmed |
Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection |
| title_sort |
Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection |
| author |
Marques, Filipe |
| author_facet |
Marques, Filipe Vale-Costa, Sílvia Cruz, Tânia Marques, Joana Moreira Silva, Tânia Neves, João Vilares Cortes, Sofia Fernandes, Ana Rocha, Eduardo Appelberg, Rui Rodrigues, Pedro Tomás, Ana M. Gomes, Maria Salomé |
| author_role |
author |
| author2 |
Vale-Costa, Sílvia Cruz, Tânia Marques, Joana Moreira Silva, Tânia Neves, João Vilares Cortes, Sofia Fernandes, Ana Rocha, Eduardo Appelberg, Rui Rodrigues, Pedro Tomás, Ana M. Gomes, Maria Salomé |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) Instituto de Higiene e Medicina Tropical (IHMT) RUN |
| dc.contributor.author.fl_str_mv |
Marques, Filipe Vale-Costa, Sílvia Cruz, Tânia Marques, Joana Moreira Silva, Tânia Neves, João Vilares Cortes, Sofia Fernandes, Ana Rocha, Eduardo Appelberg, Rui Rodrigues, Pedro Tomás, Ana M. Gomes, Maria Salomé |
| dc.subject.por.fl_str_mv |
Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
| topic |
Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
| description |
PMID: 26684322 WOS:000367080800004 |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-12-18 2015-12-18T00:00:00Z 2018-05-11T22:02:55Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.1186/s13071-015-1259-6 |
| url |
https://doi.org/10.1186/s13071-015-1259-6 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
PURE: 1729028 http://www.scopus.com/inward/record.url?scp=84950313576&partnerID=8YFLogxK https://doi.org/10.1186/s13071-015-1259-6 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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