Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/42316 |
Resumo: | [Excerpt] Background: The colonization of indwelling medical devices by biofilm-forming bacteria is one of the major causes of healthcare-associated infections (Percival et al., 2015). Staphylococcus epidermidis, a biofilm-forming commensal bacterium that inhabits human skin and mucosae, is considered one of most important causes of medical devices-related infections, being particularly associated with the use of intravascular catheters (Mack et al., 2013). Although S. epidermidis biofilms are classically associated with the development of chronic infections (Costerton et al., 1999), the release of cells from the biofilm has been associated with onset of acute infections such as embolic events of endocarditis (Pitz et al., 2011), bacteremia, or even septicemia (Cole et al., 2016). Bloodstream infections caused by S. epidermidis are typically indolent and difficult to eradicate significantly increasing patient’s morbidity (Kleinschmidt et al., 2015) and mortality among immunocompromised (Khashu et al., 2006) and immunosuppressed patients (Bender and Hughes,1980).Inaddition,thecostsassociatedwiththediagnosisandtreatmentofthesesecondary infections is estimated to be approximately $20,000 per occurrence (Kilgore and Brossette, 2008). Henceforth, it is imperative to redefine strategies for the management of the pathologic events associated with biofilm disassembly. Since bloodstream infections are one of the most frequent complications caused by S. epidermidis biofilm disassembly (Cole et al., 2016), a comprehensive analysis of the interplay between S. epidermidis biofilm-released cells (BRC) and hosts’ blood components would be invaluable. Herein, as the first step toward the understanding of this interaction,wehavecharacterized,usingRNAsequencing(RNAseq)technology,thetranscriptome of S. epidermidis BRC upon interaction with whole human blood, polymorphonuclear, or mononuclear leukocytes and plasma. |
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Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factorsStaphylococcus epidermidis biofilmsbiofilm-released cellshuman bloodhuman plasmahuman leukocytestranscriptomeScience & Technology[Excerpt] Background: The colonization of indwelling medical devices by biofilm-forming bacteria is one of the major causes of healthcare-associated infections (Percival et al., 2015). Staphylococcus epidermidis, a biofilm-forming commensal bacterium that inhabits human skin and mucosae, is considered one of most important causes of medical devices-related infections, being particularly associated with the use of intravascular catheters (Mack et al., 2013). Although S. epidermidis biofilms are classically associated with the development of chronic infections (Costerton et al., 1999), the release of cells from the biofilm has been associated with onset of acute infections such as embolic events of endocarditis (Pitz et al., 2011), bacteremia, or even septicemia (Cole et al., 2016). Bloodstream infections caused by S. epidermidis are typically indolent and difficult to eradicate significantly increasing patient’s morbidity (Kleinschmidt et al., 2015) and mortality among immunocompromised (Khashu et al., 2006) and immunosuppressed patients (Bender and Hughes,1980).Inaddition,thecostsassociatedwiththediagnosisandtreatmentofthesesecondary infections is estimated to be approximately $20,000 per occurrence (Kilgore and Brossette, 2008). Henceforth, it is imperative to redefine strategies for the management of the pathologic events associated with biofilm disassembly. Since bloodstream infections are one of the most frequent complications caused by S. epidermidis biofilm disassembly (Cole et al., 2016), a comprehensive analysis of the interplay between S. epidermidis biofilm-released cells (BRC) and hosts’ blood components would be invaluable. Herein, as the first step toward the understanding of this interaction,wehavecharacterized,usingRNAsequencing(RNAseq)technology,thetranscriptome of S. epidermidis BRC upon interaction with whole human blood, polymorphonuclear, or mononuclear leukocytes and plasma.This study was funded by the Portuguese Foundation for Science and Technology (FCT) by the project with the reference FCOMP-01-012014-FEDER-041246 (EXPL/BIA-MIC/0101/2013), the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the. scope of Norte2020 - Programa Operational Regional do Norte. NC is an Investigador FCT. AT is supported by the FCT fellowship SFRH/BPD/99961/2014. The hinders had no role in study design, data collection and interpretation, or decision to submit the work for publication.Frontiers MediaUniversidade do MinhoFrança, Ângela Maria Oliveira SousaPier, G. B.Vilanova, M.Cerca, Nuno2016-07-212016-07-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/42316engFrança, Angela; Pier, G. B.; Vilanova, M.; Cerca N, Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors. Frontiers in Microbiology, 7(1143), 2016.1664-302X1664-302X10.3389/fmicb.2016.01143http://journal.frontiersin.org/journal/microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:18Zoai:repositorium.sdum.uminho.pt:1822/42316Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:32.354715Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors |
title |
Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors |
spellingShingle |
Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors França, Ângela Maria Oliveira Sousa Staphylococcus epidermidis biofilms biofilm-released cells human blood human plasma human leukocytes transcriptome Science & Technology |
title_short |
Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors |
title_full |
Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors |
title_fullStr |
Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors |
title_full_unstemmed |
Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors |
title_sort |
Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors |
author |
França, Ângela Maria Oliveira Sousa |
author_facet |
França, Ângela Maria Oliveira Sousa Pier, G. B. Vilanova, M. Cerca, Nuno |
author_role |
author |
author2 |
Pier, G. B. Vilanova, M. Cerca, Nuno |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
França, Ângela Maria Oliveira Sousa Pier, G. B. Vilanova, M. Cerca, Nuno |
dc.subject.por.fl_str_mv |
Staphylococcus epidermidis biofilms biofilm-released cells human blood human plasma human leukocytes transcriptome Science & Technology |
topic |
Staphylococcus epidermidis biofilms biofilm-released cells human blood human plasma human leukocytes transcriptome Science & Technology |
description |
[Excerpt] Background: The colonization of indwelling medical devices by biofilm-forming bacteria is one of the major causes of healthcare-associated infections (Percival et al., 2015). Staphylococcus epidermidis, a biofilm-forming commensal bacterium that inhabits human skin and mucosae, is considered one of most important causes of medical devices-related infections, being particularly associated with the use of intravascular catheters (Mack et al., 2013). Although S. epidermidis biofilms are classically associated with the development of chronic infections (Costerton et al., 1999), the release of cells from the biofilm has been associated with onset of acute infections such as embolic events of endocarditis (Pitz et al., 2011), bacteremia, or even septicemia (Cole et al., 2016). Bloodstream infections caused by S. epidermidis are typically indolent and difficult to eradicate significantly increasing patient’s morbidity (Kleinschmidt et al., 2015) and mortality among immunocompromised (Khashu et al., 2006) and immunosuppressed patients (Bender and Hughes,1980).Inaddition,thecostsassociatedwiththediagnosisandtreatmentofthesesecondary infections is estimated to be approximately $20,000 per occurrence (Kilgore and Brossette, 2008). Henceforth, it is imperative to redefine strategies for the management of the pathologic events associated with biofilm disassembly. Since bloodstream infections are one of the most frequent complications caused by S. epidermidis biofilm disassembly (Cole et al., 2016), a comprehensive analysis of the interplay between S. epidermidis biofilm-released cells (BRC) and hosts’ blood components would be invaluable. Herein, as the first step toward the understanding of this interaction,wehavecharacterized,usingRNAsequencing(RNAseq)technology,thetranscriptome of S. epidermidis BRC upon interaction with whole human blood, polymorphonuclear, or mononuclear leukocytes and plasma. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-21 2016-07-21T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/42316 |
url |
http://hdl.handle.net/1822/42316 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
França, Angela; Pier, G. B.; Vilanova, M.; Cerca N, Transcriptomic analysis of Staphylococcus epidermidis biofilm-released cells upon interaction with human blood circulating immune cells and soluble factors. Frontiers in Microbiology, 7(1143), 2016. 1664-302X 1664-302X 10.3389/fmicb.2016.01143 http://journal.frontiersin.org/journal/microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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