Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/12562 |
Resumo: | Extracts and compounds derived from marine organisms have reportedly shown some osteogenic potential. As such, these bioactives may aid in the treatment of musculoskeletal conditions such as osteoporosis; helping to address inefficacies with current treatment options. In this study, 72 fractions were tested for their in vitro osteogenic activity using a human foetal osteoblast (hFOB) cell line and bone marrow derived mesenchymal stem cells (MSCs), focusing on their cytotoxic, proliferative and differentiation effects. Extracts dissolved in dimethyl sulfoxide and ethanol showed no significant osteogenic potential. However, two extracts derived from powder residues (left over from original organic extractions) caused a significant promotion of MSC differentiation. Bioactivity from powder residues derived from the epiphytic red algae Ceramium pallidum is described in detail to highlight its treatment potential. In vitro, C. pallidum was shown to promote MSC differentiation and extracellular matrix mineralisation. In vivo, this extract caused a significant increase in opercular bone growth of zebrafish larvae and a significant increase in bone density of regenerated adult caudal fins. Our findings therefore show the importance of continued screening efforts, particularly of novel extract sources, and the presence of bioactive compounds in C. pallidum extract. |
id |
RCAP_d60327a24beee6cf0f389220511ea655 |
---|---|
oai_identifier_str |
oai:sapientia.ualg.pt:10400.1/12562 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidumAquatic organismsCell differentiationCell proliferationFetusHumansOsteoblastsOsteogenesisRhodophytaPlant extractsExtracts and compounds derived from marine organisms have reportedly shown some osteogenic potential. As such, these bioactives may aid in the treatment of musculoskeletal conditions such as osteoporosis; helping to address inefficacies with current treatment options. In this study, 72 fractions were tested for their in vitro osteogenic activity using a human foetal osteoblast (hFOB) cell line and bone marrow derived mesenchymal stem cells (MSCs), focusing on their cytotoxic, proliferative and differentiation effects. Extracts dissolved in dimethyl sulfoxide and ethanol showed no significant osteogenic potential. However, two extracts derived from powder residues (left over from original organic extractions) caused a significant promotion of MSC differentiation. Bioactivity from powder residues derived from the epiphytic red algae Ceramium pallidum is described in detail to highlight its treatment potential. In vitro, C. pallidum was shown to promote MSC differentiation and extracellular matrix mineralisation. In vivo, this extract caused a significant increase in opercular bone growth of zebrafish larvae and a significant increase in bone density of regenerated adult caudal fins. Our findings therefore show the importance of continued screening efforts, particularly of novel extract sources, and the presence of bioactive compounds in C. pallidum extract.Agência financiadora / Número do subsídio Marine Institute under the Marine Research Sub-Programme of the Irish National Development Plan 2007-2013 Santander (Bank, UK) Engineering and Physical Sciences Research Council (EPSRC) EP/M027473/1Public Library of ScienceSapientiaCarson, Matthew A.Nelson, JohnCancela, M. LeonorLaizé, VincentGavaia, PauloRae, MargaretHeesch, SvenjaVerzin, EugeneGilmore, Brendan F.Clarke, Susan A.2019-05-24T10:05:55Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/12562eng1932-620310.1371/journal.pone.0207303info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:24:32Zoai:sapientia.ualg.pt:10400.1/12562Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:53.685667Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum |
title |
Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum |
spellingShingle |
Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum Carson, Matthew A. Aquatic organisms Cell differentiation Cell proliferation Fetus Humans Osteoblasts Osteogenesis Rhodophyta Plant extracts |
title_short |
Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum |
title_full |
Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum |
title_fullStr |
Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum |
title_full_unstemmed |
Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum |
title_sort |
Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum |
author |
Carson, Matthew A. |
author_facet |
Carson, Matthew A. Nelson, John Cancela, M. Leonor Laizé, Vincent Gavaia, Paulo Rae, Margaret Heesch, Svenja Verzin, Eugene Gilmore, Brendan F. Clarke, Susan A. |
author_role |
author |
author2 |
Nelson, John Cancela, M. Leonor Laizé, Vincent Gavaia, Paulo Rae, Margaret Heesch, Svenja Verzin, Eugene Gilmore, Brendan F. Clarke, Susan A. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Carson, Matthew A. Nelson, John Cancela, M. Leonor Laizé, Vincent Gavaia, Paulo Rae, Margaret Heesch, Svenja Verzin, Eugene Gilmore, Brendan F. Clarke, Susan A. |
dc.subject.por.fl_str_mv |
Aquatic organisms Cell differentiation Cell proliferation Fetus Humans Osteoblasts Osteogenesis Rhodophyta Plant extracts |
topic |
Aquatic organisms Cell differentiation Cell proliferation Fetus Humans Osteoblasts Osteogenesis Rhodophyta Plant extracts |
description |
Extracts and compounds derived from marine organisms have reportedly shown some osteogenic potential. As such, these bioactives may aid in the treatment of musculoskeletal conditions such as osteoporosis; helping to address inefficacies with current treatment options. In this study, 72 fractions were tested for their in vitro osteogenic activity using a human foetal osteoblast (hFOB) cell line and bone marrow derived mesenchymal stem cells (MSCs), focusing on their cytotoxic, proliferative and differentiation effects. Extracts dissolved in dimethyl sulfoxide and ethanol showed no significant osteogenic potential. However, two extracts derived from powder residues (left over from original organic extractions) caused a significant promotion of MSC differentiation. Bioactivity from powder residues derived from the epiphytic red algae Ceramium pallidum is described in detail to highlight its treatment potential. In vitro, C. pallidum was shown to promote MSC differentiation and extracellular matrix mineralisation. In vivo, this extract caused a significant increase in opercular bone growth of zebrafish larvae and a significant increase in bone density of regenerated adult caudal fins. Our findings therefore show the importance of continued screening efforts, particularly of novel extract sources, and the presence of bioactive compounds in C. pallidum extract. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z 2019-05-24T10:05:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/12562 |
url |
http://hdl.handle.net/10400.1/12562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 10.1371/journal.pone.0207303 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133273746571264 |