3d-hepatocyte culture applied to parasitology
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/116512 |
Resumo: | The application of innovative three-dimensional (3D) spheroids cell culture strategy to Parasitology offers the opportunity to closely explore host–parasite interactions. Here we present a first report on the application of 3D hepatic spheroids to unravel the immune response of canine hepatocytes exposed to Leishmania infantum. The liver, usually considered a major metabolic organ, also performs several important immunological functions and constitutes a target organ for L. infantum infection, the etiological agent of canine leishmaniasis (CanL), and a parasitic disease of major veterinary and public health concern. 3D hepatic spheroids were able to sense and immunologically react to L. infantum parasites, generating an innate immune response by increasing nitric oxide (NO) production and enhancing toll-like receptor (TLR) 2 and interleukin-10 gene expression. The immune response orchestrated by canine hepatocytes also lead to the impairment of several cytochrome P450 (CYP450) with possible implications for liver natural xenobiotic metabolization capacity. The application of meglumine antimoniate (MgA) increased the inflammatory response of 3D hepatic spheroids by inducing the expression of Nucleotide oligomerization domain (NOD)-like receptors 1 and NOD2 and TLR2, TLR4, and TLR9 and enhancing gene expression of tumour necrosis factor α. It is therefore suggested that hepatocytes are key effector cells and can activate and orchestrate the immune response to L. infantum parasites. |
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7160 |
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3d-hepatocyte culture applied to parasitologyimmune activation of canine hepatic spheroids exposed to leishmania infantum3D cell cultureHepatocytesImmune responseLeishmania infantumMeglumine antimoniateMedicine (miscellaneous)Biochemistry, Genetics and Molecular Biology(all)SDG 3 - Good Health and Well-beingThe application of innovative three-dimensional (3D) spheroids cell culture strategy to Parasitology offers the opportunity to closely explore host–parasite interactions. Here we present a first report on the application of 3D hepatic spheroids to unravel the immune response of canine hepatocytes exposed to Leishmania infantum. The liver, usually considered a major metabolic organ, also performs several important immunological functions and constitutes a target organ for L. infantum infection, the etiological agent of canine leishmaniasis (CanL), and a parasitic disease of major veterinary and public health concern. 3D hepatic spheroids were able to sense and immunologically react to L. infantum parasites, generating an innate immune response by increasing nitric oxide (NO) production and enhancing toll-like receptor (TLR) 2 and interleukin-10 gene expression. The immune response orchestrated by canine hepatocytes also lead to the impairment of several cytochrome P450 (CYP450) with possible implications for liver natural xenobiotic metabolization capacity. The application of meglumine antimoniate (MgA) increased the inflammatory response of 3D hepatic spheroids by inducing the expression of Nucleotide oligomerization domain (NOD)-like receptors 1 and NOD2 and TLR2, TLR4, and TLR9 and enhancing gene expression of tumour necrosis factor α. It is therefore suggested that hepatocytes are key effector cells and can activate and orchestrate the immune response to L. infantum parasites.Vector borne diseases and pathogens (VBD)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNRodrigues, Armanda V.Alexandre-Pires, GraçaValério-Bolas, AnaSantos-Mateus, DavidRafael-Fernandes, MarianaPereira, Maria A.Ligeiro, DárioNunes, TelmoAlves-Azevedo, RaquelSantos, MarcosFonseca, Isabel Pereira daSantos-Gomes, Gabriela2021-04-30T22:43:56Z2020-12-182020-12-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21application/pdfhttp://hdl.handle.net/10362/116512eng2227-9059PURE: 27513717https://doi.org/10.3390/biomedicines8120628info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-09T01:37:48Zoai:run.unl.pt:10362/116512Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-09T01:37:48Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
3d-hepatocyte culture applied to parasitology immune activation of canine hepatic spheroids exposed to leishmania infantum |
title |
3d-hepatocyte culture applied to parasitology |
spellingShingle |
3d-hepatocyte culture applied to parasitology Rodrigues, Armanda V. 3D cell culture Hepatocytes Immune response Leishmania infantum Meglumine antimoniate Medicine (miscellaneous) Biochemistry, Genetics and Molecular Biology(all) SDG 3 - Good Health and Well-being |
title_short |
3d-hepatocyte culture applied to parasitology |
title_full |
3d-hepatocyte culture applied to parasitology |
title_fullStr |
3d-hepatocyte culture applied to parasitology |
title_full_unstemmed |
3d-hepatocyte culture applied to parasitology |
title_sort |
3d-hepatocyte culture applied to parasitology |
author |
Rodrigues, Armanda V. |
author_facet |
Rodrigues, Armanda V. Alexandre-Pires, Graça Valério-Bolas, Ana Santos-Mateus, David Rafael-Fernandes, Mariana Pereira, Maria A. Ligeiro, Dário Nunes, Telmo Alves-Azevedo, Raquel Santos, Marcos Fonseca, Isabel Pereira da Santos-Gomes, Gabriela |
author_role |
author |
author2 |
Alexandre-Pires, Graça Valério-Bolas, Ana Santos-Mateus, David Rafael-Fernandes, Mariana Pereira, Maria A. Ligeiro, Dário Nunes, Telmo Alves-Azevedo, Raquel Santos, Marcos Fonseca, Isabel Pereira da Santos-Gomes, Gabriela |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Vector borne diseases and pathogens (VBD) Global Health and Tropical Medicine (GHTM) Instituto de Higiene e Medicina Tropical (IHMT) RUN |
dc.contributor.author.fl_str_mv |
Rodrigues, Armanda V. Alexandre-Pires, Graça Valério-Bolas, Ana Santos-Mateus, David Rafael-Fernandes, Mariana Pereira, Maria A. Ligeiro, Dário Nunes, Telmo Alves-Azevedo, Raquel Santos, Marcos Fonseca, Isabel Pereira da Santos-Gomes, Gabriela |
dc.subject.por.fl_str_mv |
3D cell culture Hepatocytes Immune response Leishmania infantum Meglumine antimoniate Medicine (miscellaneous) Biochemistry, Genetics and Molecular Biology(all) SDG 3 - Good Health and Well-being |
topic |
3D cell culture Hepatocytes Immune response Leishmania infantum Meglumine antimoniate Medicine (miscellaneous) Biochemistry, Genetics and Molecular Biology(all) SDG 3 - Good Health and Well-being |
description |
The application of innovative three-dimensional (3D) spheroids cell culture strategy to Parasitology offers the opportunity to closely explore host–parasite interactions. Here we present a first report on the application of 3D hepatic spheroids to unravel the immune response of canine hepatocytes exposed to Leishmania infantum. The liver, usually considered a major metabolic organ, also performs several important immunological functions and constitutes a target organ for L. infantum infection, the etiological agent of canine leishmaniasis (CanL), and a parasitic disease of major veterinary and public health concern. 3D hepatic spheroids were able to sense and immunologically react to L. infantum parasites, generating an innate immune response by increasing nitric oxide (NO) production and enhancing toll-like receptor (TLR) 2 and interleukin-10 gene expression. The immune response orchestrated by canine hepatocytes also lead to the impairment of several cytochrome P450 (CYP450) with possible implications for liver natural xenobiotic metabolization capacity. The application of meglumine antimoniate (MgA) increased the inflammatory response of 3D hepatic spheroids by inducing the expression of Nucleotide oligomerization domain (NOD)-like receptors 1 and NOD2 and TLR2, TLR4, and TLR9 and enhancing gene expression of tumour necrosis factor α. It is therefore suggested that hepatocytes are key effector cells and can activate and orchestrate the immune response to L. infantum parasites. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-18 2020-12-18T00:00:00Z 2021-04-30T22:43:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/116512 |
url |
http://hdl.handle.net/10362/116512 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2227-9059 PURE: 27513717 https://doi.org/10.3390/biomedicines8120628 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
21 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545793555398656 |