3d-hepatocyte culture applied to parasitology

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Armanda V.
Data de Publicação: 2020
Outros Autores: Alexandre-Pires, Graça, Valério-Bolas, Ana, Santos-Mateus, David, Rafael-Fernandes, Mariana, Pereira, Maria A., Ligeiro, Dário, Nunes, Telmo, Alves-Azevedo, Raquel, Santos, Marcos, Fonseca, Isabel Pereira da, Santos-Gomes, Gabriela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116512
Resumo: The application of innovative three-dimensional (3D) spheroids cell culture strategy to Parasitology offers the opportunity to closely explore host–parasite interactions. Here we present a first report on the application of 3D hepatic spheroids to unravel the immune response of canine hepatocytes exposed to Leishmania infantum. The liver, usually considered a major metabolic organ, also performs several important immunological functions and constitutes a target organ for L. infantum infection, the etiological agent of canine leishmaniasis (CanL), and a parasitic disease of major veterinary and public health concern. 3D hepatic spheroids were able to sense and immunologically react to L. infantum parasites, generating an innate immune response by increasing nitric oxide (NO) production and enhancing toll-like receptor (TLR) 2 and interleukin-10 gene expression. The immune response orchestrated by canine hepatocytes also lead to the impairment of several cytochrome P450 (CYP450) with possible implications for liver natural xenobiotic metabolization capacity. The application of meglumine antimoniate (MgA) increased the inflammatory response of 3D hepatic spheroids by inducing the expression of Nucleotide oligomerization domain (NOD)-like receptors 1 and NOD2 and TLR2, TLR4, and TLR9 and enhancing gene expression of tumour necrosis factor α. It is therefore suggested that hepatocytes are key effector cells and can activate and orchestrate the immune response to L. infantum parasites.
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spelling 3d-hepatocyte culture applied to parasitologyimmune activation of canine hepatic spheroids exposed to leishmania infantum3D cell cultureHepatocytesImmune responseLeishmania infantumMeglumine antimoniateMedicine (miscellaneous)Biochemistry, Genetics and Molecular Biology(all)SDG 3 - Good Health and Well-beingThe application of innovative three-dimensional (3D) spheroids cell culture strategy to Parasitology offers the opportunity to closely explore host–parasite interactions. Here we present a first report on the application of 3D hepatic spheroids to unravel the immune response of canine hepatocytes exposed to Leishmania infantum. The liver, usually considered a major metabolic organ, also performs several important immunological functions and constitutes a target organ for L. infantum infection, the etiological agent of canine leishmaniasis (CanL), and a parasitic disease of major veterinary and public health concern. 3D hepatic spheroids were able to sense and immunologically react to L. infantum parasites, generating an innate immune response by increasing nitric oxide (NO) production and enhancing toll-like receptor (TLR) 2 and interleukin-10 gene expression. The immune response orchestrated by canine hepatocytes also lead to the impairment of several cytochrome P450 (CYP450) with possible implications for liver natural xenobiotic metabolization capacity. The application of meglumine antimoniate (MgA) increased the inflammatory response of 3D hepatic spheroids by inducing the expression of Nucleotide oligomerization domain (NOD)-like receptors 1 and NOD2 and TLR2, TLR4, and TLR9 and enhancing gene expression of tumour necrosis factor α. It is therefore suggested that hepatocytes are key effector cells and can activate and orchestrate the immune response to L. infantum parasites.Vector borne diseases and pathogens (VBD)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNRodrigues, Armanda V.Alexandre-Pires, GraçaValério-Bolas, AnaSantos-Mateus, DavidRafael-Fernandes, MarianaPereira, Maria A.Ligeiro, DárioNunes, TelmoAlves-Azevedo, RaquelSantos, MarcosFonseca, Isabel Pereira daSantos-Gomes, Gabriela2021-04-30T22:43:56Z2020-12-182020-12-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21application/pdfhttp://hdl.handle.net/10362/116512eng2227-9059PURE: 27513717https://doi.org/10.3390/biomedicines8120628info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-09T01:37:48Zoai:run.unl.pt:10362/116512Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-09T01:37:48Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv 3d-hepatocyte culture applied to parasitology
immune activation of canine hepatic spheroids exposed to leishmania infantum
title 3d-hepatocyte culture applied to parasitology
spellingShingle 3d-hepatocyte culture applied to parasitology
Rodrigues, Armanda V.
3D cell culture
Hepatocytes
Immune response
Leishmania infantum
Meglumine antimoniate
Medicine (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
SDG 3 - Good Health and Well-being
title_short 3d-hepatocyte culture applied to parasitology
title_full 3d-hepatocyte culture applied to parasitology
title_fullStr 3d-hepatocyte culture applied to parasitology
title_full_unstemmed 3d-hepatocyte culture applied to parasitology
title_sort 3d-hepatocyte culture applied to parasitology
author Rodrigues, Armanda V.
author_facet Rodrigues, Armanda V.
Alexandre-Pires, Graça
Valério-Bolas, Ana
Santos-Mateus, David
Rafael-Fernandes, Mariana
Pereira, Maria A.
Ligeiro, Dário
Nunes, Telmo
Alves-Azevedo, Raquel
Santos, Marcos
Fonseca, Isabel Pereira da
Santos-Gomes, Gabriela
author_role author
author2 Alexandre-Pires, Graça
Valério-Bolas, Ana
Santos-Mateus, David
Rafael-Fernandes, Mariana
Pereira, Maria A.
Ligeiro, Dário
Nunes, Telmo
Alves-Azevedo, Raquel
Santos, Marcos
Fonseca, Isabel Pereira da
Santos-Gomes, Gabriela
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Vector borne diseases and pathogens (VBD)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Rodrigues, Armanda V.
Alexandre-Pires, Graça
Valério-Bolas, Ana
Santos-Mateus, David
Rafael-Fernandes, Mariana
Pereira, Maria A.
Ligeiro, Dário
Nunes, Telmo
Alves-Azevedo, Raquel
Santos, Marcos
Fonseca, Isabel Pereira da
Santos-Gomes, Gabriela
dc.subject.por.fl_str_mv 3D cell culture
Hepatocytes
Immune response
Leishmania infantum
Meglumine antimoniate
Medicine (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
SDG 3 - Good Health and Well-being
topic 3D cell culture
Hepatocytes
Immune response
Leishmania infantum
Meglumine antimoniate
Medicine (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
SDG 3 - Good Health and Well-being
description The application of innovative three-dimensional (3D) spheroids cell culture strategy to Parasitology offers the opportunity to closely explore host–parasite interactions. Here we present a first report on the application of 3D hepatic spheroids to unravel the immune response of canine hepatocytes exposed to Leishmania infantum. The liver, usually considered a major metabolic organ, also performs several important immunological functions and constitutes a target organ for L. infantum infection, the etiological agent of canine leishmaniasis (CanL), and a parasitic disease of major veterinary and public health concern. 3D hepatic spheroids were able to sense and immunologically react to L. infantum parasites, generating an innate immune response by increasing nitric oxide (NO) production and enhancing toll-like receptor (TLR) 2 and interleukin-10 gene expression. The immune response orchestrated by canine hepatocytes also lead to the impairment of several cytochrome P450 (CYP450) with possible implications for liver natural xenobiotic metabolization capacity. The application of meglumine antimoniate (MgA) increased the inflammatory response of 3D hepatic spheroids by inducing the expression of Nucleotide oligomerization domain (NOD)-like receptors 1 and NOD2 and TLR2, TLR4, and TLR9 and enhancing gene expression of tumour necrosis factor α. It is therefore suggested that hepatocytes are key effector cells and can activate and orchestrate the immune response to L. infantum parasites.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-18
2020-12-18T00:00:00Z
2021-04-30T22:43:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116512
url http://hdl.handle.net/10362/116512
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2227-9059
PURE: 27513717
https://doi.org/10.3390/biomedicines8120628
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 21
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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