Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/63305 |
Resumo: | In a multicellular organism, the extracellular matrix (ECM) provides a cell-supporting scaffold and helps maintaining the biophysical integrity of tissues and organs. At the same time it plays crucial roles in cellular communication and signalling, with implications in spatial organisation, motility and differentiation. Similarly, the presence of an ECM-like extracellular polymeric substance is known to support and protect bacterial and fungal multicellular aggregates, such as biofilms or colonies. However, the roles and composition of this microbial ECM are still poorly understood. |
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Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomicsCandida albicansChemistry Techniques, AnalyticalExtracellular MatrixFungal ProteinsGlycomicsPolysaccharidesProteomicsReproducibility of ResultsSaccharomyces cerevisiaeScience & TechnologyIn a multicellular organism, the extracellular matrix (ECM) provides a cell-supporting scaffold and helps maintaining the biophysical integrity of tissues and organs. At the same time it plays crucial roles in cellular communication and signalling, with implications in spatial organisation, motility and differentiation. Similarly, the presence of an ECM-like extracellular polymeric substance is known to support and protect bacterial and fungal multicellular aggregates, such as biofilms or colonies. However, the roles and composition of this microbial ECM are still poorly understood.Authors would like to acknowledge Joana Tulha for assistance on yeasts overlay photographs, and to Rui Armada for C. albicans ECM SDS-PAGE experiment. The proteomic analysis was carried out at the proteomics facility UCM-PCM, a member of the ProteoRed network. The polysaccharide analysis was performed at the Laboratory of Glycoconjugates Biochemistry and Cellular Biology, UFRJ, Brazil. Fabio Faria-Oliveira was supported by a PhD scholarship SFRH/BD/45368/2008 from FCT (Fundacao para a Ciencia e a Tecnologia). This work was funded by Marie Curie Initial Training Network GLYCOPHARM (PITN-GA-2012-317297), and by FCT/MEC through Portuguese funds (PIDDAC) - PEst-OE/BIA/UI4050/2014. The authors would also like to acknowledge Hugh S. Johnson for critical reading of the manuscript regarding English usage.BMCUniversidade do MinhoFaria-Oliveira, FábioCarvalho, JoanaBelmiro, Celso L RMartinez-Gomariz, MontserratHernaez, Maria LuisaPavão, MauroGil, ConchaLucas, CândidaFerreira, Célia2014-10-252014-10-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/63305eng1471-218010.1186/s12866-014-0244-025344425info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:06Zoai:repositorium.sdum.uminho.pt:1822/63305Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:51.140824Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics |
title |
Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics |
spellingShingle |
Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics Faria-Oliveira, Fábio Candida albicans Chemistry Techniques, Analytical Extracellular Matrix Fungal Proteins Glycomics Polysaccharides Proteomics Reproducibility of Results Saccharomyces cerevisiae Science & Technology |
title_short |
Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics |
title_full |
Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics |
title_fullStr |
Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics |
title_full_unstemmed |
Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics |
title_sort |
Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics |
author |
Faria-Oliveira, Fábio |
author_facet |
Faria-Oliveira, Fábio Carvalho, Joana Belmiro, Celso L R Martinez-Gomariz, Montserrat Hernaez, Maria Luisa Pavão, Mauro Gil, Concha Lucas, Cândida Ferreira, Célia |
author_role |
author |
author2 |
Carvalho, Joana Belmiro, Celso L R Martinez-Gomariz, Montserrat Hernaez, Maria Luisa Pavão, Mauro Gil, Concha Lucas, Cândida Ferreira, Célia |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Faria-Oliveira, Fábio Carvalho, Joana Belmiro, Celso L R Martinez-Gomariz, Montserrat Hernaez, Maria Luisa Pavão, Mauro Gil, Concha Lucas, Cândida Ferreira, Célia |
dc.subject.por.fl_str_mv |
Candida albicans Chemistry Techniques, Analytical Extracellular Matrix Fungal Proteins Glycomics Polysaccharides Proteomics Reproducibility of Results Saccharomyces cerevisiae Science & Technology |
topic |
Candida albicans Chemistry Techniques, Analytical Extracellular Matrix Fungal Proteins Glycomics Polysaccharides Proteomics Reproducibility of Results Saccharomyces cerevisiae Science & Technology |
description |
In a multicellular organism, the extracellular matrix (ECM) provides a cell-supporting scaffold and helps maintaining the biophysical integrity of tissues and organs. At the same time it plays crucial roles in cellular communication and signalling, with implications in spatial organisation, motility and differentiation. Similarly, the presence of an ECM-like extracellular polymeric substance is known to support and protect bacterial and fungal multicellular aggregates, such as biofilms or colonies. However, the roles and composition of this microbial ECM are still poorly understood. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-25 2014-10-25T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/63305 |
url |
http://hdl.handle.net/1822/63305 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1471-2180 10.1186/s12866-014-0244-0 25344425 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
BMC |
publisher.none.fl_str_mv |
BMC |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132700919988224 |