Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics

Detalhes bibliográficos
Autor(a) principal: Faria-Oliveira, Fábio
Data de Publicação: 2014
Outros Autores: Carvalho, Joana, Belmiro, Celso L R, Martinez-Gomariz, Montserrat, Hernaez, Maria Luisa, Pavão, Mauro, Gil, Concha, Lucas, Cândida, Ferreira, Célia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/63305
Resumo: In a multicellular organism, the extracellular matrix (ECM) provides a cell-supporting scaffold and helps maintaining the biophysical integrity of tissues and organs. At the same time it plays crucial roles in cellular communication and signalling, with implications in spatial organisation, motility and differentiation. Similarly, the presence of an ECM-like extracellular polymeric substance is known to support and protect bacterial and fungal multicellular aggregates, such as biofilms or colonies. However, the roles and composition of this microbial ECM are still poorly understood.
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spelling Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomicsCandida albicansChemistry Techniques, AnalyticalExtracellular MatrixFungal ProteinsGlycomicsPolysaccharidesProteomicsReproducibility of ResultsSaccharomyces cerevisiaeScience & TechnologyIn a multicellular organism, the extracellular matrix (ECM) provides a cell-supporting scaffold and helps maintaining the biophysical integrity of tissues and organs. At the same time it plays crucial roles in cellular communication and signalling, with implications in spatial organisation, motility and differentiation. Similarly, the presence of an ECM-like extracellular polymeric substance is known to support and protect bacterial and fungal multicellular aggregates, such as biofilms or colonies. However, the roles and composition of this microbial ECM are still poorly understood.Authors would like to acknowledge Joana Tulha for assistance on yeasts overlay photographs, and to Rui Armada for C. albicans ECM SDS-PAGE experiment. The proteomic analysis was carried out at the proteomics facility UCM-PCM, a member of the ProteoRed network. The polysaccharide analysis was performed at the Laboratory of Glycoconjugates Biochemistry and Cellular Biology, UFRJ, Brazil. Fabio Faria-Oliveira was supported by a PhD scholarship SFRH/BD/45368/2008 from FCT (Fundacao para a Ciencia e a Tecnologia). This work was funded by Marie Curie Initial Training Network GLYCOPHARM (PITN-GA-2012-317297), and by FCT/MEC through Portuguese funds (PIDDAC) - PEst-OE/BIA/UI4050/2014. The authors would also like to acknowledge Hugh S. Johnson for critical reading of the manuscript regarding English usage.BMCUniversidade do MinhoFaria-Oliveira, FábioCarvalho, JoanaBelmiro, Celso L RMartinez-Gomariz, MontserratHernaez, Maria LuisaPavão, MauroGil, ConchaLucas, CândidaFerreira, Célia2014-10-252014-10-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/63305eng1471-218010.1186/s12866-014-0244-025344425info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:06Zoai:repositorium.sdum.uminho.pt:1822/63305Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:51.140824Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics
title Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics
spellingShingle Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics
Faria-Oliveira, Fábio
Candida albicans
Chemistry Techniques, Analytical
Extracellular Matrix
Fungal Proteins
Glycomics
Polysaccharides
Proteomics
Reproducibility of Results
Saccharomyces cerevisiae
Science & Technology
title_short Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics
title_full Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics
title_fullStr Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics
title_full_unstemmed Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics
title_sort Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics
author Faria-Oliveira, Fábio
author_facet Faria-Oliveira, Fábio
Carvalho, Joana
Belmiro, Celso L R
Martinez-Gomariz, Montserrat
Hernaez, Maria Luisa
Pavão, Mauro
Gil, Concha
Lucas, Cândida
Ferreira, Célia
author_role author
author2 Carvalho, Joana
Belmiro, Celso L R
Martinez-Gomariz, Montserrat
Hernaez, Maria Luisa
Pavão, Mauro
Gil, Concha
Lucas, Cândida
Ferreira, Célia
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Faria-Oliveira, Fábio
Carvalho, Joana
Belmiro, Celso L R
Martinez-Gomariz, Montserrat
Hernaez, Maria Luisa
Pavão, Mauro
Gil, Concha
Lucas, Cândida
Ferreira, Célia
dc.subject.por.fl_str_mv Candida albicans
Chemistry Techniques, Analytical
Extracellular Matrix
Fungal Proteins
Glycomics
Polysaccharides
Proteomics
Reproducibility of Results
Saccharomyces cerevisiae
Science & Technology
topic Candida albicans
Chemistry Techniques, Analytical
Extracellular Matrix
Fungal Proteins
Glycomics
Polysaccharides
Proteomics
Reproducibility of Results
Saccharomyces cerevisiae
Science & Technology
description In a multicellular organism, the extracellular matrix (ECM) provides a cell-supporting scaffold and helps maintaining the biophysical integrity of tissues and organs. At the same time it plays crucial roles in cellular communication and signalling, with implications in spatial organisation, motility and differentiation. Similarly, the presence of an ECM-like extracellular polymeric substance is known to support and protect bacterial and fungal multicellular aggregates, such as biofilms or colonies. However, the roles and composition of this microbial ECM are still poorly understood.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-25
2014-10-25T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/63305
url http://hdl.handle.net/1822/63305
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1471-2180
10.1186/s12866-014-0244-0
25344425
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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