Atomic force microscopy-based force spectroscopy - biological and biomedical applications

Detalhes bibliográficos
Autor(a) principal: Carvalho, Filomena A.
Data de Publicação: 2012
Outros Autores: Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10766
Resumo: © 2012 IUBMB
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spelling Atomic force microscopy-based force spectroscopy - biological and biomedical applicationsAtomic force microscopyForce spectroscopyBiological applicationsBiomedical applicationsErythrocyteFibrinogen© 2012 IUBMBThe use of atomic force microscopy (AFM) applied to biological systems to generate high resolution images is gaining a wider acceptance. However, the most remarkable advances are being achieved on the use of the AFM to measure inter- and intramolecular interaction forces with piconewton resolution, not only to demonstrate this ability but also actually to solve biological and biomedical relevant questions. Single-molecule force spectroscopy recognition studies enable the detection of specific interaction forces, based on the AFM sensitivity and the possibility of manipulating individual molecules. In this review, we describe the basic principles of this methodology and some of the practical aspects involved. The ability to measure interactions at the single-molecule level is illustrated by some relevant examples. A special focus is given to the study of the fibrinogen–erythrocyte binding and its relevance as a cardiovascular risk factor. An approach to the latter problem by single-molecule force spectroscopy allowed the molecular recognition, characterization, and partial identification of a previously unknown receptor for fibrinogen on human erythrocytes.This work was supported by Fundação para a Ciência e a Tecnologia—Ministério do Ensino e Ciência (FCT-MEC, Portugal) and by Fundação Calouste Gulbenkian (Portugal).WileyRepositório da Universidade de LisboaCarvalho, Filomena A.Santos, Nuno C.2014-03-21T14:30:18Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10766engIUBMB Life, 64(6): 465–472, June 20121521-6543http://dx.doi.org/10.1002/iub.1037metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:14:44Zoai:repositorio.ul.pt:10451/10766Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:14:44Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Atomic force microscopy-based force spectroscopy - biological and biomedical applications
title Atomic force microscopy-based force spectroscopy - biological and biomedical applications
spellingShingle Atomic force microscopy-based force spectroscopy - biological and biomedical applications
Carvalho, Filomena A.
Atomic force microscopy
Force spectroscopy
Biological applications
Biomedical applications
Erythrocyte
Fibrinogen
title_short Atomic force microscopy-based force spectroscopy - biological and biomedical applications
title_full Atomic force microscopy-based force spectroscopy - biological and biomedical applications
title_fullStr Atomic force microscopy-based force spectroscopy - biological and biomedical applications
title_full_unstemmed Atomic force microscopy-based force spectroscopy - biological and biomedical applications
title_sort Atomic force microscopy-based force spectroscopy - biological and biomedical applications
author Carvalho, Filomena A.
author_facet Carvalho, Filomena A.
Santos, Nuno C.
author_role author
author2 Santos, Nuno C.
author2_role author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Filomena A.
Santos, Nuno C.
dc.subject.por.fl_str_mv Atomic force microscopy
Force spectroscopy
Biological applications
Biomedical applications
Erythrocyte
Fibrinogen
topic Atomic force microscopy
Force spectroscopy
Biological applications
Biomedical applications
Erythrocyte
Fibrinogen
description © 2012 IUBMB
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-21T14:30:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10766
url http://hdl.handle.net/10451/10766
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv IUBMB Life, 64(6): 465–472, June 2012
1521-6543
http://dx.doi.org/10.1002/iub.1037
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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