Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality

Detalhes bibliográficos
Autor(a) principal: Carvalho, Filomena A.
Data de Publicação: 2013
Outros Autores: Martins, Ivo C., Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10769
Resumo: © 2012 Elsevier Inc. All rights reserved.
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spelling Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionalityAtomic force microscopySingle-molecule force spectroscopyProtein unfoldingProtein stretchingIntramolecular forces© 2012 Elsevier Inc. All rights reserved.Atomic force microscopy (AFM) applied to biological systems can, besides generating high-quality and well-resolved images, be employed to study protein folding via AFM-based force spectroscopy. This approach allowed remarkable advances in the measurement of inter- and intramolecular interaction forces with piconewton resolution. The detection of specific interaction forces between molecules based on the AFM sensitivity and the manipulation of individual molecules greatly advanced the understanding of intra-protein and protein–ligand interactions. Apart from the academic interest in the resolution of basic scientific questions, this technique has also key importance on the clarification of several biological questions of immediate biomedical relevance. Force spectroscopy is an especially appropriate technique for ‘‘mechanical proteins’’ that can provide crucial information on single protein molecules and/or domains. Importantly, it also has the potential of combining in a single experiment spatial and kinetic measurements. Here, the main principles of this methodology are described, after which the ability to measure interactions at the single-molecule level is discussed, in the context of relevant protein-folding examples. We intend to demonstrate the potential of AFM-based force spectroscopy in the study of protein folding, especially since this technique is able to circumvent some of the difficulties typically encountered in classical thermal/chemical denaturation studies.This work was supported by Fundação para a Ciência e a Tecnologia – Ministério do Ensino e Ciência (FCT–MEC, Portugal) and by Fundação Calouste Gulbenkian (Portugal). I.C.M. also acknowledges FCT–MEC fellowship SFRH/BPD/74287/2010.ElsevierRepositório da Universidade de LisboaCarvalho, Filomena A.Martins, Ivo C.Santos, Nuno C.2014-03-21T14:44:22Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10769engArchives of Biochemistry and Biophysics 531 (2013) 116–1270003-9861http://dx.doi.org/10.1016/j.abb.2012.11.007metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:14:42Zoai:repositorio.ul.pt:10451/10769Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:14:42Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality
title Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality
spellingShingle Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality
Carvalho, Filomena A.
Atomic force microscopy
Single-molecule force spectroscopy
Protein unfolding
Protein stretching
Intramolecular forces
title_short Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality
title_full Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality
title_fullStr Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality
title_full_unstemmed Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality
title_sort Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality
author Carvalho, Filomena A.
author_facet Carvalho, Filomena A.
Martins, Ivo C.
Santos, Nuno C.
author_role author
author2 Martins, Ivo C.
Santos, Nuno C.
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Filomena A.
Martins, Ivo C.
Santos, Nuno C.
dc.subject.por.fl_str_mv Atomic force microscopy
Single-molecule force spectroscopy
Protein unfolding
Protein stretching
Intramolecular forces
topic Atomic force microscopy
Single-molecule force spectroscopy
Protein unfolding
Protein stretching
Intramolecular forces
description © 2012 Elsevier Inc. All rights reserved.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-03-21T14:44:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10769
url http://hdl.handle.net/10451/10769
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Biochemistry and Biophysics 531 (2013) 116–127
0003-9861
http://dx.doi.org/10.1016/j.abb.2012.11.007
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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