Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/1351 |
Resumo: | Six hundred twenty-one samples from Portugal, the Cabo Verde archipelago, and Guinea-Bissau were typed for HLA-A, HLA-B, and HLADRB1usingthepolymerasechainreaction–sequence-specificoligonucleotide probe (PCR-SSOP) method and the sequence-based typing (SBT) method to characterizeandcomparediscrepanciesbetweenthetwomethods.Fifty-three alleles (4.27% of 1,242 chromosomes typed) identified by the PCR-SSOP method were not concordant with the results obtained using the SBT method. Thirty-four (2.74% of total chromosomes typed) PCR-SSOP mistyping results were discrepancies inside the same allele group and 19 others (1.53% of total chromosomes typed) were relative to nonconcordant results between different groups. PCR-SSOP allele mistyping is the result of interpretation difficulties resulting from less intense, absent, or dubious hybridization patterns. Noncommercial PCR-SSOP procedures are highly exigent on the technicians’ experience and the availability of properly calibrated high-precision equipment. |
id |
RCAP_dce4273bb97b096710dfb95d42f48cdc |
---|---|
oai_identifier_str |
oai:digituma.uma.pt:10400.13/1351 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case studyHLAPolymerase chain reaction - sequence-specific - oligonucleotide probe (PCR-SSOP) techniqueSequence-based typing (SBT) techniquePortugalCabo VerdeGuiné-BissauAzores islands (Portugal).Six hundred twenty-one samples from Portugal, the Cabo Verde archipelago, and Guinea-Bissau were typed for HLA-A, HLA-B, and HLADRB1usingthepolymerasechainreaction–sequence-specificoligonucleotide probe (PCR-SSOP) method and the sequence-based typing (SBT) method to characterizeandcomparediscrepanciesbetweenthetwomethods.Fifty-three alleles (4.27% of 1,242 chromosomes typed) identified by the PCR-SSOP method were not concordant with the results obtained using the SBT method. Thirty-four (2.74% of total chromosomes typed) PCR-SSOP mistyping results were discrepancies inside the same allele group and 19 others (1.53% of total chromosomes typed) were relative to nonconcordant results between different groups. PCR-SSOP allele mistyping is the result of interpretation difficulties resulting from less intense, absent, or dubious hybridization patterns. Noncommercial PCR-SSOP procedures are highly exigent on the technicians’ experience and the availability of properly calibrated high-precision equipment.Wayne State University PressDigitUMaSpínola, HélderBruges-Armas, JácomeBrehm, António2017-01-26T16:29:50Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/1351engSpínola, H., Bruges-Armas, J., & Brehm, A. (2007). Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: A Case Study. Human Biology, 79 (5), 537-543.0018-7143info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-02T05:42:48Zoai:digituma.uma.pt:10400.13/1351Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:04:04.605732Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study |
title |
Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study |
spellingShingle |
Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study Spínola, Hélder HLA Polymerase chain reaction - sequence-specific - oligonucleotide probe (PCR-SSOP) technique Sequence-based typing (SBT) technique Portugal Cabo Verde Guiné-Bissau Azores islands (Portugal) . |
title_short |
Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study |
title_full |
Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study |
title_fullStr |
Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study |
title_full_unstemmed |
Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study |
title_sort |
Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study |
author |
Spínola, Hélder |
author_facet |
Spínola, Hélder Bruges-Armas, Jácome Brehm, António |
author_role |
author |
author2 |
Bruges-Armas, Jácome Brehm, António |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Spínola, Hélder Bruges-Armas, Jácome Brehm, António |
dc.subject.por.fl_str_mv |
HLA Polymerase chain reaction - sequence-specific - oligonucleotide probe (PCR-SSOP) technique Sequence-based typing (SBT) technique Portugal Cabo Verde Guiné-Bissau Azores islands (Portugal) . |
topic |
HLA Polymerase chain reaction - sequence-specific - oligonucleotide probe (PCR-SSOP) technique Sequence-based typing (SBT) technique Portugal Cabo Verde Guiné-Bissau Azores islands (Portugal) . |
description |
Six hundred twenty-one samples from Portugal, the Cabo Verde archipelago, and Guinea-Bissau were typed for HLA-A, HLA-B, and HLADRB1usingthepolymerasechainreaction–sequence-specificoligonucleotide probe (PCR-SSOP) method and the sequence-based typing (SBT) method to characterizeandcomparediscrepanciesbetweenthetwomethods.Fifty-three alleles (4.27% of 1,242 chromosomes typed) identified by the PCR-SSOP method were not concordant with the results obtained using the SBT method. Thirty-four (2.74% of total chromosomes typed) PCR-SSOP mistyping results were discrepancies inside the same allele group and 19 others (1.53% of total chromosomes typed) were relative to nonconcordant results between different groups. PCR-SSOP allele mistyping is the result of interpretation difficulties resulting from less intense, absent, or dubious hybridization patterns. Noncommercial PCR-SSOP procedures are highly exigent on the technicians’ experience and the availability of properly calibrated high-precision equipment. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 2007-01-01T00:00:00Z 2017-01-26T16:29:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/1351 |
url |
http://hdl.handle.net/10400.13/1351 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Spínola, H., Bruges-Armas, J., & Brehm, A. (2007). Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: A Case Study. Human Biology, 79 (5), 537-543. 0018-7143 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wayne State University Press |
publisher.none.fl_str_mv |
Wayne State University Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799129910960193536 |