PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.7/896 |
Resumo: | The deposited article version is the Epub Ahead of Print version of the article (the "Accepted Manuscript"), posted online 20th September 2018, provided by Company of Biologists. It has peer-review. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formationPLK4MTOCsin vitro reconstitutionmicrotubule nucleationPCMcentrosomesde novosupramolecular assembliesThe deposited article version is the Epub Ahead of Print version of the article (the "Accepted Manuscript"), posted online 20th September 2018, provided by Company of Biologists. It has peer-review.The deposited article version contains attached the supplementary materials within the pdf.The centrosome is an important microtubule-organizing centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by the pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to an existing structure (canonical duplication) or de novo How centrosomes form de novo is not known. The master driver of centrosome biogenesis, PLK4, is critical to recruit several centriole components. Here, we investigate the beginning of centrosome biogenesis, taking advantage of Xenopus egg extracts, where PLK4 can induce de novo MTOC formation (Eckerdt et al., 2011; Zitouni et al., 2016). Surprisingly, we observe that in vitro, PLK4 can self-assemble into condensates that recruit α/β-tubulin. In Xenopus extracts, PLK4 assemblies additionally recruit PLK4's substrate, STIL, and the microtubule nucleator, γ-tubulin, forming acentriolar MTOCs de novo The assembly of these robust microtubule asters is independent of dynein, similarly to centrosomes. We suggest a new mechanism of action for PLK4, where it forms a self-organizing catalytic scaffold that recruits centriole components, PCM factors and α/β-tubulin, leading to MTOC formation.We are thankful to Anna Akhmanova, Raquel Oliveira and Jeffrey B.Woodruff for reading and discussing the manuscript. We are also thankful to Catarina Nabais for the GFP control construct and Vladimir Joukov for the Xenopus Cep192 antibody. S.M.G was funded by an EMBO Long term fellowship ALTF 1088-2009, a Marie curie Intra-European fellowship (#253373) and a FCT postdoctoral fellowship. The collaboration with J.L. laboratory in the USA was supported by a The Company of Biologists travel grant. S.Z is funded by ERC grant ERC-COG-683258. Research in JL lab was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research. M.B-D. Laboratory is supported by an ERC grant ERC-COG-683258 and FCT Investigator to MBD.Company of BiologistsARCAGouveia, Susana MontenegroZitouni, SihemKong, DongDuarte, PauloGomes, Beatriz FerreiraSousa, Ana LauraTranfield, Erin M.Hyman, AnthonyLoncarek, JadrankaBettencourt-Dias, Monica2019-09-01T00:30:08Z2018-09-202018-09-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/896enghttp://jcs.biologists.org/content/early/2018/09/17/jcs.219501.long10.1242/jcs.219501info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:18Zoai:arca.igc.gulbenkian.pt:10400.7/896Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:12:06.277637Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formation |
| title |
PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formation |
| spellingShingle |
PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formation Gouveia, Susana Montenegro PLK4 MTOCs in vitro reconstitution microtubule nucleation PCM centrosomes de novo supramolecular assemblies |
| title_short |
PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formation |
| title_full |
PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formation |
| title_fullStr |
PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formation |
| title_full_unstemmed |
PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formation |
| title_sort |
PLK4 is a microtubule-associated protein that self assembles promoting de novo MTOC formation |
| author |
Gouveia, Susana Montenegro |
| author_facet |
Gouveia, Susana Montenegro Zitouni, Sihem Kong, Dong Duarte, Paulo Gomes, Beatriz Ferreira Sousa, Ana Laura Tranfield, Erin M. Hyman, Anthony Loncarek, Jadranka Bettencourt-Dias, Monica |
| author_role |
author |
| author2 |
Zitouni, Sihem Kong, Dong Duarte, Paulo Gomes, Beatriz Ferreira Sousa, Ana Laura Tranfield, Erin M. Hyman, Anthony Loncarek, Jadranka Bettencourt-Dias, Monica |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
ARCA |
| dc.contributor.author.fl_str_mv |
Gouveia, Susana Montenegro Zitouni, Sihem Kong, Dong Duarte, Paulo Gomes, Beatriz Ferreira Sousa, Ana Laura Tranfield, Erin M. Hyman, Anthony Loncarek, Jadranka Bettencourt-Dias, Monica |
| dc.subject.por.fl_str_mv |
PLK4 MTOCs in vitro reconstitution microtubule nucleation PCM centrosomes de novo supramolecular assemblies |
| topic |
PLK4 MTOCs in vitro reconstitution microtubule nucleation PCM centrosomes de novo supramolecular assemblies |
| description |
The deposited article version is the Epub Ahead of Print version of the article (the "Accepted Manuscript"), posted online 20th September 2018, provided by Company of Biologists. It has peer-review. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-09-20 2018-09-20T00:00:00Z 2019-09-01T00:30:08Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/896 |
| url |
http://hdl.handle.net/10400.7/896 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
http://jcs.biologists.org/content/early/2018/09/17/jcs.219501.long 10.1242/jcs.219501 |
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info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Company of Biologists |
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Company of Biologists |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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