Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS method

Detalhes bibliográficos
Autor(a) principal: Lopes, Daniel Deodato
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/9318
Resumo: Dissertation to obtain the Master Degree in Biotechnology
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spelling Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS methodMicrobubblesSupercritical fluidsPGSSPFCGas encapsulationDissertation to obtain the Master Degree in BiotechnologyThe main goal of this Project was the development of microbubbles (MBs) and gas-filled porous particles (GPPs) by supercritical fluids (SCF) processes, namely particles from gas saturated solution (PGSS). MBs of perfluorcarbons (PFC) are currently used as contrast agents in ultrasound imaging and as ultrasound-guided drug delivery for targeted therapeutic applications. They are normally produced by processes that present some limitations. The SCF processes are an innovative technology for the production of these MB’s that has not yet been exploited and can overcome these limitations. Carriers are used to prevent rapid diffusion of the gas out of the particle. In the first part of this thesis, a preliminary selection of carrier materials was carried out, including lipids of different hydrophilic-lipophilic balance (HLB) and hydrophilic polymers. Their behavior in the presence of PFC and mixtures of PFC and sc-CO2 was qualitatively studied. A lipid of high HLB value, Gelucire 50/13, was selected to produce the GPPs by PGSS. Main parameters of the process, temperature and pressure, were studied in the range from 55ºC to 80ºC and from 8,5 MPa to 15,4 MPa. Besides, the carrier:PFC ratio and the molecular structure of the PFC were investigated. Particle morphology was analyzed by Scanning Electron Microscopy (SEM), and the presence of PFC was determined by Nuclear Magnetic Resonance (NMR). Small and non agglomerated particles were obtained at 80ºC and 8.5 MPa. Cyclic C4F8 was entrapped in lipid particles in higher amount than of linear C3F8. Nevertheless, the stability of particles obtained at the best operating conditions (80ºC and 8.5 MPa), was relatively short (below 3h) being the majority of the gas released in the first hour.Faculdade de Ciências e TecnologiaDuarte, CatarinaRUNLopes, Daniel Deodato2013-04-15T10:37:40Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/9318TID:201755580enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:42:18Zoai:run.unl.pt:10362/9318Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:42.162914Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS method
title Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS method
spellingShingle Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS method
Lopes, Daniel Deodato
Microbubbles
Supercritical fluids
PGSS
PFC
Gas encapsulation
title_short Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS method
title_full Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS method
title_fullStr Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS method
title_full_unstemmed Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS method
title_sort Preparation of gas-filled porous microparticles (GPPs) and microbubbles (MBs) by PGSS method
author Lopes, Daniel Deodato
author_facet Lopes, Daniel Deodato
author_role author
dc.contributor.none.fl_str_mv Duarte, Catarina
RUN
dc.contributor.author.fl_str_mv Lopes, Daniel Deodato
dc.subject.por.fl_str_mv Microbubbles
Supercritical fluids
PGSS
PFC
Gas encapsulation
topic Microbubbles
Supercritical fluids
PGSS
PFC
Gas encapsulation
description Dissertation to obtain the Master Degree in Biotechnology
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2013-04-15T10:37:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/9318
TID:201755580
url http://hdl.handle.net/10362/9318
identifier_str_mv TID:201755580
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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