Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants

Detalhes bibliográficos
Autor(a) principal: Franklin, Gregory
Data de Publicação: 2006
Outros Autores: Arvinth, S., Sheeba, Caroline J., Kanchana, M., Subramonian, N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/10494
Resumo: We have developed a new, simple, quick and genotype-independent method for direct regeneration of sugarcane using novel midrib segment explants. Our protocol involves two steps: the pretreatment of starting material on MS (Murashige and Skoog (1962) Physiol Plant 15:473–497) medium containing 3.0 mg/l 2,4- dichlorophenoxyacetic acid (2,4-D) for 8 days under continuous dark and subsequent transfer of the explants to MS medium augmented with 0.1 mg/l benzyladenine (BA) and 0.1 mg/l naphthaleneacetic acid (NAA) under light-dark conditions. On the regeneration medium, numerous globular structures appeared from the explants and subsequently differentiated into shoots. Regenerated shoots attained 2–5 cm height within 30 days of culture initiation and readily rooted on MS basal medium. Hardened plants were successfully established in the greenhouse. The regulation of sugarcane morphogenesis by auxin pretreatment is discussed.
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spelling Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explantsAuxin pretreatmentDirect regenerationGlobular structuresMidrib segmentsSugarcaneScience & TechnologyWe have developed a new, simple, quick and genotype-independent method for direct regeneration of sugarcane using novel midrib segment explants. Our protocol involves two steps: the pretreatment of starting material on MS (Murashige and Skoog (1962) Physiol Plant 15:473–497) medium containing 3.0 mg/l 2,4- dichlorophenoxyacetic acid (2,4-D) for 8 days under continuous dark and subsequent transfer of the explants to MS medium augmented with 0.1 mg/l benzyladenine (BA) and 0.1 mg/l naphthaleneacetic acid (NAA) under light-dark conditions. On the regeneration medium, numerous globular structures appeared from the explants and subsequently differentiated into shoots. Regenerated shoots attained 2–5 cm height within 30 days of culture initiation and readily rooted on MS basal medium. Hardened plants were successfully established in the greenhouse. The regulation of sugarcane morphogenesis by auxin pretreatment is discussed.SpringerUniversidade do MinhoFranklin, GregoryArvinth, S.Sheeba, Caroline J.Kanchana, M.Subramonian, N.20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/10494eng“Plant Growth Regulation”. ISSN 0167-6903. 50:2-3 (2006) 111–119.0167-690310.1007/s10725-006-9108-4www.springerlink.cominfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:17Zoai:repositorium.sdum.uminho.pt:1822/10494Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:11:05.623119Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants
title Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants
spellingShingle Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants
Franklin, Gregory
Auxin pretreatment
Direct regeneration
Globular structures
Midrib segments
Sugarcane
Science & Technology
title_short Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants
title_full Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants
title_fullStr Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants
title_full_unstemmed Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants
title_sort Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants
author Franklin, Gregory
author_facet Franklin, Gregory
Arvinth, S.
Sheeba, Caroline J.
Kanchana, M.
Subramonian, N.
author_role author
author2 Arvinth, S.
Sheeba, Caroline J.
Kanchana, M.
Subramonian, N.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Franklin, Gregory
Arvinth, S.
Sheeba, Caroline J.
Kanchana, M.
Subramonian, N.
dc.subject.por.fl_str_mv Auxin pretreatment
Direct regeneration
Globular structures
Midrib segments
Sugarcane
Science & Technology
topic Auxin pretreatment
Direct regeneration
Globular structures
Midrib segments
Sugarcane
Science & Technology
description We have developed a new, simple, quick and genotype-independent method for direct regeneration of sugarcane using novel midrib segment explants. Our protocol involves two steps: the pretreatment of starting material on MS (Murashige and Skoog (1962) Physiol Plant 15:473–497) medium containing 3.0 mg/l 2,4- dichlorophenoxyacetic acid (2,4-D) for 8 days under continuous dark and subsequent transfer of the explants to MS medium augmented with 0.1 mg/l benzyladenine (BA) and 0.1 mg/l naphthaleneacetic acid (NAA) under light-dark conditions. On the regeneration medium, numerous globular structures appeared from the explants and subsequently differentiated into shoots. Regenerated shoots attained 2–5 cm height within 30 days of culture initiation and readily rooted on MS basal medium. Hardened plants were successfully established in the greenhouse. The regulation of sugarcane morphogenesis by auxin pretreatment is discussed.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/10494
url http://hdl.handle.net/1822/10494
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv “Plant Growth Regulation”. ISSN 0167-6903. 50:2-3 (2006) 111–119.
0167-6903
10.1007/s10725-006-9108-4
www.springerlink.com
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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