Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters

Detalhes bibliográficos
Autor(a) principal: Chiesa, Stefania
Data de Publicação: 2015
Outros Autores: Scalici, Massimiliano, Lucentini, Livia, Marzano, Francesco Nonnis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/16818
Resumo: Although the symbionts of non-vertebrate metazoans have received scarce attention, scientific interest has recently increased as these symbionts can spread diseases and alter the diversity of the ecosystem where hosts are introduced. We report here a symbiont newly described by molecular markers and observed on the yabby Cherax destructor Clark, 1936, collected in Italian freshwaters. Preliminary morphological observations were performed and a species identification was proposed using molecular techniques. In particular, a 644-bp long 28S rDNA fragment was successfully sequenced and could be aligned for the entire sample set. The minimum spanning network identified two haplotypes clustered into a single haplogroup (H1) together with Temnosewellia minor Haswell, 1888 reference sequences. Considering the introduction of temnocephalans, this represents the third discovery of alien crayfish symbionts in Italian freshwaters, but the first obtained by molecular data. Outside Europe, there are only few records of alien temnocephalans worldwide, introduced with the crayfishes from Australia. The occurrence of this new temnocephalid species and the great number of potential hosts species inhabiting Italian freshwaters suggest that the Italian peninsula might have many undescribed temnocephalid populations/species.
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spelling Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwatersTemnocephalidaalien speciessymbiontsmolecular identification28S rDNAItalyAlthough the symbionts of non-vertebrate metazoans have received scarce attention, scientific interest has recently increased as these symbionts can spread diseases and alter the diversity of the ecosystem where hosts are introduced. We report here a symbiont newly described by molecular markers and observed on the yabby Cherax destructor Clark, 1936, collected in Italian freshwaters. Preliminary morphological observations were performed and a species identification was proposed using molecular techniques. In particular, a 644-bp long 28S rDNA fragment was successfully sequenced and could be aligned for the entire sample set. The minimum spanning network identified two haplotypes clustered into a single haplogroup (H1) together with Temnosewellia minor Haswell, 1888 reference sequences. Considering the introduction of temnocephalans, this represents the third discovery of alien crayfish symbionts in Italian freshwaters, but the first obtained by molecular data. Outside Europe, there are only few records of alien temnocephalans worldwide, introduced with the crayfishes from Australia. The occurrence of this new temnocephalid species and the great number of potential hosts species inhabiting Italian freshwaters suggest that the Italian peninsula might have many undescribed temnocephalid populations/species.Regional Euro-Asian Biological Invasions Centre (REABIC)2017-02-15T12:57:20Z2015-01-01T00:00:00Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/16818eng1798-654010.3391/ai.2015.10.2.09Chiesa, StefaniaScalici, MassimilianoLucentini, LiviaMarzano, Francesco Nonnisinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:31:24Zoai:ria.ua.pt:10773/16818Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:51:51.191428Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters
title Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters
spellingShingle Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters
Chiesa, Stefania
Temnocephalida
alien species
symbionts
molecular identification
28S rDNA
Italy
title_short Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters
title_full Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters
title_fullStr Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters
title_full_unstemmed Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters
title_sort Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters
author Chiesa, Stefania
author_facet Chiesa, Stefania
Scalici, Massimiliano
Lucentini, Livia
Marzano, Francesco Nonnis
author_role author
author2 Scalici, Massimiliano
Lucentini, Livia
Marzano, Francesco Nonnis
author2_role author
author
author
dc.contributor.author.fl_str_mv Chiesa, Stefania
Scalici, Massimiliano
Lucentini, Livia
Marzano, Francesco Nonnis
dc.subject.por.fl_str_mv Temnocephalida
alien species
symbionts
molecular identification
28S rDNA
Italy
topic Temnocephalida
alien species
symbionts
molecular identification
28S rDNA
Italy
description Although the symbionts of non-vertebrate metazoans have received scarce attention, scientific interest has recently increased as these symbionts can spread diseases and alter the diversity of the ecosystem where hosts are introduced. We report here a symbiont newly described by molecular markers and observed on the yabby Cherax destructor Clark, 1936, collected in Italian freshwaters. Preliminary morphological observations were performed and a species identification was proposed using molecular techniques. In particular, a 644-bp long 28S rDNA fragment was successfully sequenced and could be aligned for the entire sample set. The minimum spanning network identified two haplotypes clustered into a single haplogroup (H1) together with Temnosewellia minor Haswell, 1888 reference sequences. Considering the introduction of temnocephalans, this represents the third discovery of alien crayfish symbionts in Italian freshwaters, but the first obtained by molecular data. Outside Europe, there are only few records of alien temnocephalans worldwide, introduced with the crayfishes from Australia. The occurrence of this new temnocephalid species and the great number of potential hosts species inhabiting Italian freshwaters suggest that the Italian peninsula might have many undescribed temnocephalid populations/species.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01T00:00:00Z
2015
2017-02-15T12:57:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/16818
url http://hdl.handle.net/10773/16818
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1798-6540
10.3391/ai.2015.10.2.09
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Regional Euro-Asian Biological Invasions Centre (REABIC)
publisher.none.fl_str_mv Regional Euro-Asian Biological Invasions Centre (REABIC)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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