Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/28958 |
Resumo: | The Camellia genus comprises a vast array of underexplored medicinal plants that merit a systematic valorization to exploit their potential as natural sources of phytochemicals with associated health-promoting properties. In this work, flower extracts from eight poorly characterized Camellia japonica L. cultivars were subjected to polyphenol profiling through untargeted metabolomics combined with in vitro functional analysis. Anthocyanins, mostly represented by cyanidin 3-O-glycosides, flavones, and flavonols, were found as the major constituents of C. japonica flowers, together with hydroxycinnamic acids, tyrosols, alkylphenols, and stilbenes, which were detected for the first time in this species. The application of multivariate statistics revealed a flower colordependent fingerprint of C. japonica cultivars, featuring anthocyanins and other flavonoids as metabolite markers associated with color-flowered cultivars with respect to white-flowered ones. The accumulation of anthocyanins, especially reported in ‘Eugenia de Montijo’ flowers, was highly correlated with the cytotoxic and anti-inflammatory properties of the derived extracts, including AGS, Caco-2, and MCF7 cancer cell lines. Moreover, the flavones accumulation reported in ‘Carolyn Tuttle’ extracts was also associated with high rates of free-radical scavenging activity, as well as a potent cytotoxicity against the Caco-2 cell line. In general, C. japonica anthocyanin-enriched flower extracts were revealed as promising candidates for the industrial production of polyphenols with associated biological activities of high interest for critical sectors in the food, pharmaceutical, and cosmetic industries. |
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Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial applicationPhenolic compoundsMultivariate statisticsBioactivitiesMedicinal plantsAntioxidantsPhytochemicalsThe Camellia genus comprises a vast array of underexplored medicinal plants that merit a systematic valorization to exploit their potential as natural sources of phytochemicals with associated health-promoting properties. In this work, flower extracts from eight poorly characterized Camellia japonica L. cultivars were subjected to polyphenol profiling through untargeted metabolomics combined with in vitro functional analysis. Anthocyanins, mostly represented by cyanidin 3-O-glycosides, flavones, and flavonols, were found as the major constituents of C. japonica flowers, together with hydroxycinnamic acids, tyrosols, alkylphenols, and stilbenes, which were detected for the first time in this species. The application of multivariate statistics revealed a flower colordependent fingerprint of C. japonica cultivars, featuring anthocyanins and other flavonoids as metabolite markers associated with color-flowered cultivars with respect to white-flowered ones. The accumulation of anthocyanins, especially reported in ‘Eugenia de Montijo’ flowers, was highly correlated with the cytotoxic and anti-inflammatory properties of the derived extracts, including AGS, Caco-2, and MCF7 cancer cell lines. Moreover, the flavones accumulation reported in ‘Carolyn Tuttle’ extracts was also associated with high rates of free-radical scavenging activity, as well as a potent cytotoxicity against the Caco-2 cell line. In general, C. japonica anthocyanin-enriched flower extracts were revealed as promising candidates for the industrial production of polyphenols with associated biological activities of high interest for critical sectors in the food, pharmaceutical, and cosmetic industries.The research leading to these results was supported by MICINN supporting the Ramón y Cajal grant for M.A.-P. (RYC-2017–22891) and the Juan de la Cierva Formación grant for T.-O. (FJC2019–042549-I). The authors acknowledge Xunta de Galicia for funding the post-doctoral grant of L. C. (ED481B-2021/152) and the program EXCELENCIAED431F 2020/12, which supported the work by F.C. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020), and national funding by FCT, P.I., through the institutional scientific employment program contract for L.-B. and R. C.-C. The work by P.G.-P. was financed by the Spanish Ministry of Universities under the application 33.50.460A.752 and by the European Union NextGenerationEU/PRTR through a Margarita Salas contract by the Universidade de Vigo.ElsevierBiblioteca Digital do IPBPereira, A.G.Cassani, LuciaOludemi, TaofiqChamorro, FranklinCalhelha, Ricardo C.Prieto Lage, Miguel A.Barros, LillianSimal-Gandara, JesusLucini, LuigiGarcia-Perez, Pascual2023-12-18T09:52:56Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/28958engPereira, A.G.; Cassani, Lucia; Oludemi, Taofiq; Chamorro, Franklin; Calhelha, Ricardo C.; Prieto Lage, Miguel A.; Barros, Lillian; Simal-Gandara, Jesus; Lucini, Luigi; Garcia-Perez, Pascual (2023). Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application. Industrial Crops and Products. ISSN 0926-6690. 204, p. 1-110926-669010.1016/j.indcrop.2023.117389info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-20T01:19:17Zoai:bibliotecadigital.ipb.pt:10198/28958Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:55:06.196414Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application |
title |
Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application |
spellingShingle |
Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application Pereira, A.G. Phenolic compounds Multivariate statistics Bioactivities Medicinal plants Antioxidants Phytochemicals |
title_short |
Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application |
title_full |
Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application |
title_fullStr |
Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application |
title_full_unstemmed |
Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application |
title_sort |
Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application |
author |
Pereira, A.G. |
author_facet |
Pereira, A.G. Cassani, Lucia Oludemi, Taofiq Chamorro, Franklin Calhelha, Ricardo C. Prieto Lage, Miguel A. Barros, Lillian Simal-Gandara, Jesus Lucini, Luigi Garcia-Perez, Pascual |
author_role |
author |
author2 |
Cassani, Lucia Oludemi, Taofiq Chamorro, Franklin Calhelha, Ricardo C. Prieto Lage, Miguel A. Barros, Lillian Simal-Gandara, Jesus Lucini, Luigi Garcia-Perez, Pascual |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Pereira, A.G. Cassani, Lucia Oludemi, Taofiq Chamorro, Franklin Calhelha, Ricardo C. Prieto Lage, Miguel A. Barros, Lillian Simal-Gandara, Jesus Lucini, Luigi Garcia-Perez, Pascual |
dc.subject.por.fl_str_mv |
Phenolic compounds Multivariate statistics Bioactivities Medicinal plants Antioxidants Phytochemicals |
topic |
Phenolic compounds Multivariate statistics Bioactivities Medicinal plants Antioxidants Phytochemicals |
description |
The Camellia genus comprises a vast array of underexplored medicinal plants that merit a systematic valorization to exploit their potential as natural sources of phytochemicals with associated health-promoting properties. In this work, flower extracts from eight poorly characterized Camellia japonica L. cultivars were subjected to polyphenol profiling through untargeted metabolomics combined with in vitro functional analysis. Anthocyanins, mostly represented by cyanidin 3-O-glycosides, flavones, and flavonols, were found as the major constituents of C. japonica flowers, together with hydroxycinnamic acids, tyrosols, alkylphenols, and stilbenes, which were detected for the first time in this species. The application of multivariate statistics revealed a flower colordependent fingerprint of C. japonica cultivars, featuring anthocyanins and other flavonoids as metabolite markers associated with color-flowered cultivars with respect to white-flowered ones. The accumulation of anthocyanins, especially reported in ‘Eugenia de Montijo’ flowers, was highly correlated with the cytotoxic and anti-inflammatory properties of the derived extracts, including AGS, Caco-2, and MCF7 cancer cell lines. Moreover, the flavones accumulation reported in ‘Carolyn Tuttle’ extracts was also associated with high rates of free-radical scavenging activity, as well as a potent cytotoxicity against the Caco-2 cell line. In general, C. japonica anthocyanin-enriched flower extracts were revealed as promising candidates for the industrial production of polyphenols with associated biological activities of high interest for critical sectors in the food, pharmaceutical, and cosmetic industries. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-18T09:52:56Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/28958 |
url |
http://hdl.handle.net/10198/28958 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira, A.G.; Cassani, Lucia; Oludemi, Taofiq; Chamorro, Franklin; Calhelha, Ricardo C.; Prieto Lage, Miguel A.; Barros, Lillian; Simal-Gandara, Jesus; Lucini, Luigi; Garcia-Perez, Pascual (2023). Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application. Industrial Crops and Products. ISSN 0926-6690. 204, p. 1-11 0926-6690 10.1016/j.indcrop.2023.117389 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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