In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Production

Detalhes bibliográficos
Autor(a) principal: Wang, Panpan
Data de Publicação: 2023
Outros Autores: Frank, Anna, Appel, Jens, Boehm, Marko, Strabel, Nadine, Nowaczyk, Marc M., Schuhmann, Wolfgang, Conzuelo, Felipe, Gutekunst, Kirstin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158540
Resumo: Funding Information: P.W., A.F., and J.A. contributed equally to this work. The authors are grateful to the Bundesministerium für Bildung und Forschung (BMBF) in the framework of the project CyFun (03SF0652A). The authors also thank Prof. Wolfgang Lubitz (Max Planck Institute for Chemical Energy Conversion, Mülheim an der Ruhr) for providing the DvMF[NiFe]-H2ase used for the fabrication of the H2 microsensor. Part of the project was funded by the research training group GRK2341 “Microbial Substrate Conversion (MiCon)” of the German research council (DFG) and the Dietmar Hopp Stiftung. P.W. is grateful for the financial support provided by the China Scholarship Council (CSC). F.C. is grateful to the support provided by FCT–Fundação para a Ciência e a Tecnologia, I.P. through MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). Open access funding enabled and organized by Projekt DEAL. Funding Information: P.W., A.F., and J.A. contributed equally to this work. The authors are grateful to the Bundesministerium für Bildung und Forschung (BMBF) in the framework of the project CyFun (03SF0652A). The authors also thank Prof. Wolfgang Lubitz (Max Planck Institute for Chemical Energy Conversion, Mülheim an der Ruhr) for providing the DvMF[NiFe]‐Hase used for the fabrication of the H microsensor. Part of the project was funded by the research training group GRK2341 “Microbial Substrate Conversion (MiCon)” of the German research council (DFG) and the Dietmar Hopp Stiftung. P.W. is grateful for the financial support provided by the China Scholarship Council (CSC). F.C. is grateful to the support provided by FCT–Fundação para a Ciência e a Tecnologia, I.P. through MOSTMICRO‐ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). 2 2 Publisher Copyright: © 2023 The Authors. Advanced Energy Materials published by Wiley-VCH GmbH.
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spelling In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Productionbiophotovoltaicshydrogen evolutionhydrogenasesphotosystem Iprotein chimeraPSI–H ase fusionRenewable Energy, Sustainability and the EnvironmentMaterials Science(all)SDG 7 - Affordable and Clean EnergyFunding Information: P.W., A.F., and J.A. contributed equally to this work. The authors are grateful to the Bundesministerium für Bildung und Forschung (BMBF) in the framework of the project CyFun (03SF0652A). The authors also thank Prof. Wolfgang Lubitz (Max Planck Institute for Chemical Energy Conversion, Mülheim an der Ruhr) for providing the DvMF[NiFe]-H2ase used for the fabrication of the H2 microsensor. Part of the project was funded by the research training group GRK2341 “Microbial Substrate Conversion (MiCon)” of the German research council (DFG) and the Dietmar Hopp Stiftung. P.W. is grateful for the financial support provided by the China Scholarship Council (CSC). F.C. is grateful to the support provided by FCT–Fundação para a Ciência e a Tecnologia, I.P. through MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). Open access funding enabled and organized by Projekt DEAL. Funding Information: P.W., A.F., and J.A. contributed equally to this work. The authors are grateful to the Bundesministerium für Bildung und Forschung (BMBF) in the framework of the project CyFun (03SF0652A). The authors also thank Prof. Wolfgang Lubitz (Max Planck Institute for Chemical Energy Conversion, Mülheim an der Ruhr) for providing the DvMF[NiFe]‐Hase used for the fabrication of the H microsensor. Part of the project was funded by the research training group GRK2341 “Microbial Substrate Conversion (MiCon)” of the German research council (DFG) and the Dietmar Hopp Stiftung. P.W. is grateful for the financial support provided by the China Scholarship Council (CSC). F.C. is grateful to the support provided by FCT–Fundação para a Ciência e a Tecnologia, I.P. through MOSTMICRO‐ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). 2 2 Publisher Copyright: © 2023 The Authors. Advanced Energy Materials published by Wiley-VCH GmbH.Photosynthetic hydrogen (photoH2) production is an elegant approach to storing solar energy. The most efficient strategy is to couple the hydrogen-producing enzyme, the hydrogenase (H2ase), directly to photosystem I (PSI), which is a light-driven nanomachine found in photosynthetic organisms. PSI–H2ase fusions have been tested in vivo and in vitro. Both approaches have each their specific advantages and drawbacks. Here, a system to combine both approaches by assembling PSI–H2ase fusions in vivo for in vitro photoH2 production is established. For this, cyanobacterial PSI–H2ase fusion mutants are generated and characterized concerning photoH2 production in vivo. The chimeric protein is purified and embedded in a redox polymer on an electrode where it successfully produces photoH2 in vitro. The combination of in vivo and in vitro processes comes along with reciprocal benefits. The in vivo assembly ensures that the chimeric protein is fully functional and suited for the fabrication of bioelectrodes in vitro. At the same time, the photoelectrochemical in vitro characterization now permits to analyze the assemblies in detail. This will open avenues to optimize in vivo and in vitro approaches for photoH2 production in a target-oriented manner in the future.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNWang, PanpanFrank, AnnaAppel, JensBoehm, MarkoStrabel, NadineNowaczyk, Marc M.Schuhmann, WolfgangConzuelo, FelipeGutekunst, Kirstin2023-09-30T22:21:25Z2023-04-132023-04-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158540eng1614-6832PURE: 72623082https://doi.org/10.1002/aenm.202203232info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:05Zoai:run.unl.pt:10362/158540Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:12.073343Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Production
title In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Production
spellingShingle In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Production
Wang, Panpan
biophotovoltaics
hydrogen evolution
hydrogenases
photosystem I
protein chimera
PSI–H ase fusion
Renewable Energy, Sustainability and the Environment
Materials Science(all)
SDG 7 - Affordable and Clean Energy
title_short In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Production
title_full In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Production
title_fullStr In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Production
title_full_unstemmed In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Production
title_sort In Vivo Assembly of Photosystem I-Hydrogenase Chimera for In Vitro PhotoH2 Production
author Wang, Panpan
author_facet Wang, Panpan
Frank, Anna
Appel, Jens
Boehm, Marko
Strabel, Nadine
Nowaczyk, Marc M.
Schuhmann, Wolfgang
Conzuelo, Felipe
Gutekunst, Kirstin
author_role author
author2 Frank, Anna
Appel, Jens
Boehm, Marko
Strabel, Nadine
Nowaczyk, Marc M.
Schuhmann, Wolfgang
Conzuelo, Felipe
Gutekunst, Kirstin
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Wang, Panpan
Frank, Anna
Appel, Jens
Boehm, Marko
Strabel, Nadine
Nowaczyk, Marc M.
Schuhmann, Wolfgang
Conzuelo, Felipe
Gutekunst, Kirstin
dc.subject.por.fl_str_mv biophotovoltaics
hydrogen evolution
hydrogenases
photosystem I
protein chimera
PSI–H ase fusion
Renewable Energy, Sustainability and the Environment
Materials Science(all)
SDG 7 - Affordable and Clean Energy
topic biophotovoltaics
hydrogen evolution
hydrogenases
photosystem I
protein chimera
PSI–H ase fusion
Renewable Energy, Sustainability and the Environment
Materials Science(all)
SDG 7 - Affordable and Clean Energy
description Funding Information: P.W., A.F., and J.A. contributed equally to this work. The authors are grateful to the Bundesministerium für Bildung und Forschung (BMBF) in the framework of the project CyFun (03SF0652A). The authors also thank Prof. Wolfgang Lubitz (Max Planck Institute for Chemical Energy Conversion, Mülheim an der Ruhr) for providing the DvMF[NiFe]-H2ase used for the fabrication of the H2 microsensor. Part of the project was funded by the research training group GRK2341 “Microbial Substrate Conversion (MiCon)” of the German research council (DFG) and the Dietmar Hopp Stiftung. P.W. is grateful for the financial support provided by the China Scholarship Council (CSC). F.C. is grateful to the support provided by FCT–Fundação para a Ciência e a Tecnologia, I.P. through MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). Open access funding enabled and organized by Projekt DEAL. Funding Information: P.W., A.F., and J.A. contributed equally to this work. The authors are grateful to the Bundesministerium für Bildung und Forschung (BMBF) in the framework of the project CyFun (03SF0652A). The authors also thank Prof. Wolfgang Lubitz (Max Planck Institute for Chemical Energy Conversion, Mülheim an der Ruhr) for providing the DvMF[NiFe]‐Hase used for the fabrication of the H microsensor. Part of the project was funded by the research training group GRK2341 “Microbial Substrate Conversion (MiCon)” of the German research council (DFG) and the Dietmar Hopp Stiftung. P.W. is grateful for the financial support provided by the China Scholarship Council (CSC). F.C. is grateful to the support provided by FCT–Fundação para a Ciência e a Tecnologia, I.P. through MOSTMICRO‐ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). 2 2 Publisher Copyright: © 2023 The Authors. Advanced Energy Materials published by Wiley-VCH GmbH.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:21:25Z
2023-04-13
2023-04-13T00:00:00Z
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PURE: 72623082
https://doi.org/10.1002/aenm.202203232
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