Added value of autoregulation and multi-step kinetics of transcription initiation

Detalhes bibliográficos
Autor(a) principal: Prajapat, Mahendra Kumar
Data de Publicação: 2018
Outros Autores: Ribeiro, André S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1098/rsos.181170
Resumo: This work was supported by Tekniikan edistamissaation (M.K.P), Academy of Finland (295027 to A.S.R.), Academy of Finland Key Project Funding (305342 to A.S.R.) and Jane and Aatos Erkko Foundation (610536 to A.S.R.). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
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spelling Added value of autoregulation and multi-step kinetics of transcription initiationAutoregulation mechanismsCell-to-cell variabilityRate-limiting stepsTranscription initiationGeneralThis work was supported by Tekniikan edistamissaation (M.K.P), Academy of Finland (295027 to A.S.R.), Academy of Finland Key Project Funding (305342 to A.S.R.) and Jane and Aatos Erkko Foundation (610536 to A.S.R.). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.Bacterial gene expression regulation occurs mostly during transcription, which has two main rate-limiting steps: the close complex formation, when the RNA polymerase binds to an active promoter, and the subsequent open complex formation, after which it follows elongation. Tuning these steps’ kinetics by the action of e.g. transcription factors, allows for a wide diversity of dynamics. For example, adding autoregulation generates single-gene circuits able to perform more complex tasks. Using stochastic models of transcription kinetics with empirically validated parameter values, we investigate how autoregulation and the multi-step transcription initiation kinetics of single-gene autoregulated circuits can be combined to fine-tune steady state mean and cell-to-cell variability in protein expression levels, as well as response times. Next, we investigate how they can be jointly tuned to control complex behaviours, namely, time counting, switching dynamics and memory storage. Overall, our finding suggests that, in bacteria, jointly regulating a single-gene circuit’s topology and the transcription initiation multi-step dynamics allows enhancing complex task performance.CTS - Centro de Tecnologia e SistemasUNINOVA-Instituto de Desenvolvimento de Novas TecnologiasRUNPrajapat, Mahendra KumarRibeiro, André S.2019-06-17T22:44:44Z2018-11-012018-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1098/rsos.181170eng2054-5703PURE: 13707083http://www.scopus.com/inward/record.url?scp=85058415001&partnerID=8YFLogxKhttps://doi.org/10.1098/rsos.181170info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:33:51Zoai:run.unl.pt:10362/72896Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:16.640735Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Added value of autoregulation and multi-step kinetics of transcription initiation
title Added value of autoregulation and multi-step kinetics of transcription initiation
spellingShingle Added value of autoregulation and multi-step kinetics of transcription initiation
Prajapat, Mahendra Kumar
Autoregulation mechanisms
Cell-to-cell variability
Rate-limiting steps
Transcription initiation
General
title_short Added value of autoregulation and multi-step kinetics of transcription initiation
title_full Added value of autoregulation and multi-step kinetics of transcription initiation
title_fullStr Added value of autoregulation and multi-step kinetics of transcription initiation
title_full_unstemmed Added value of autoregulation and multi-step kinetics of transcription initiation
title_sort Added value of autoregulation and multi-step kinetics of transcription initiation
author Prajapat, Mahendra Kumar
author_facet Prajapat, Mahendra Kumar
Ribeiro, André S.
author_role author
author2 Ribeiro, André S.
author2_role author
dc.contributor.none.fl_str_mv CTS - Centro de Tecnologia e Sistemas
UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
RUN
dc.contributor.author.fl_str_mv Prajapat, Mahendra Kumar
Ribeiro, André S.
dc.subject.por.fl_str_mv Autoregulation mechanisms
Cell-to-cell variability
Rate-limiting steps
Transcription initiation
General
topic Autoregulation mechanisms
Cell-to-cell variability
Rate-limiting steps
Transcription initiation
General
description This work was supported by Tekniikan edistamissaation (M.K.P), Academy of Finland (295027 to A.S.R.), Academy of Finland Key Project Funding (305342 to A.S.R.) and Jane and Aatos Erkko Foundation (610536 to A.S.R.). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-01
2018-11-01T00:00:00Z
2019-06-17T22:44:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1098/rsos.181170
url https://doi.org/10.1098/rsos.181170
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2054-5703
PURE: 13707083
http://www.scopus.com/inward/record.url?scp=85058415001&partnerID=8YFLogxK
https://doi.org/10.1098/rsos.181170
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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