Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells

Detalhes bibliográficos
Autor(a) principal: Rodrigo, Ana P.
Data de Publicação: 2021
Outros Autores: Mendes, Vera M., Manadas, Bruno, Grosso, Ana R., Alves de Matos, António P., Baptista, Pedro V., Costa, Pedro M., Fernandes, Alexandra R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/126070
Resumo: POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013).
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spelling Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cellscancercell cyclecytotoxicityinvertebrateovarian cancerprogrammed cell deathtoxinDrug DiscoverySDG 3 - Good Health and Well-beingSDG 14 - Life Below WaterPOCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013).As Yondelis joins the ranks of approved anti-cancer drugs, the benefit from exploring the oceans' biodiversity becomes clear. From marine toxins, relevant bioproducts can be obtained due to their potential to interfere with specific pathways. We explored the cytotoxicity of toxin-bearing secretions of the polychaete Eulalia onto a battery of normal and cancer human cell lines and discovered that the cocktail of proteins is more toxic towards an ovarian cancer cell line (A2780). The secretions' main proteins were identified by proteomics and transcriptomics: 14-3-3 protein, Hsp70, Rab3, Arylsulfatase B and serine protease, the latter two being known toxins. This mixture of toxins induces cell-cycle arrest at G2/M phase after 3h exposure in A2780 cells and extrinsic programmed cell death. These findings indicate that partial re-activation of the G2/M checkpoint, which is inactivated in many cancer cells, can be partly reversed by the toxic mixture. Protein-protein interaction networks partake in two cytotoxic effects: cell-cycle arrest with a link to RAB3C and RAF1; and lytic activity of arylsulfatases. The discovery of both mechanisms indicates that venomous mixtures may affect proliferating cells in a specific manner, highlighting the cocktails' potential in the fine-tuning of anti-cancer therapeutics targeting cell cycle and protein homeostasis.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNRodrigo, Ana P.Mendes, Vera M.Manadas, BrunoGrosso, Ana R.Alves de Matos, António P.Baptista, Pedro V.Costa, Pedro M.Fernandes, Alexandra R.2021-10-13T23:19:29Z2021-01-122021-01-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/126070eng1660-3397PURE: 28346124https://doi.org/10.3390/md19010031info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:06:45Zoai:run.unl.pt:10362/126070Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:45:50.798470Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells
title Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells
spellingShingle Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells
Rodrigo, Ana P.
cancer
cell cycle
cytotoxicity
invertebrate
ovarian cancer
programmed cell death
toxin
Drug Discovery
SDG 3 - Good Health and Well-being
SDG 14 - Life Below Water
title_short Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells
title_full Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells
title_fullStr Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells
title_full_unstemmed Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells
title_sort Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells
author Rodrigo, Ana P.
author_facet Rodrigo, Ana P.
Mendes, Vera M.
Manadas, Bruno
Grosso, Ana R.
Alves de Matos, António P.
Baptista, Pedro V.
Costa, Pedro M.
Fernandes, Alexandra R.
author_role author
author2 Mendes, Vera M.
Manadas, Bruno
Grosso, Ana R.
Alves de Matos, António P.
Baptista, Pedro V.
Costa, Pedro M.
Fernandes, Alexandra R.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Rodrigo, Ana P.
Mendes, Vera M.
Manadas, Bruno
Grosso, Ana R.
Alves de Matos, António P.
Baptista, Pedro V.
Costa, Pedro M.
Fernandes, Alexandra R.
dc.subject.por.fl_str_mv cancer
cell cycle
cytotoxicity
invertebrate
ovarian cancer
programmed cell death
toxin
Drug Discovery
SDG 3 - Good Health and Well-being
SDG 14 - Life Below Water
topic cancer
cell cycle
cytotoxicity
invertebrate
ovarian cancer
programmed cell death
toxin
Drug Discovery
SDG 3 - Good Health and Well-being
SDG 14 - Life Below Water
description POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013).
publishDate 2021
dc.date.none.fl_str_mv 2021-10-13T23:19:29Z
2021-01-12
2021-01-12T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/126070
url http://hdl.handle.net/10362/126070
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1660-3397
PURE: 28346124
https://doi.org/10.3390/md19010031
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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