An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba

Detalhes bibliográficos
Autor(a) principal: Campos, Sónia
Data de Publicação: 2023
Outros Autores: Rodrigo, Ana P., Moutinho Cabral, Inês, Mendes, Vera M., Manadas, Bruno, D’Ambrosio, Mariaelena, Costa, Pedro M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/162346
Resumo: Funding Information: The Portuguese Foundation for Science and Technology (FCT), i.p., funded project WormALL (PTDC/BTA BTA/28650/2017), which includes the contract attributed to M.D. and the fellowship of A.P.R. Fundo Azul cofinanced project MARVEN (FA_05_2017_007), which includes the fellowship of I.M.C. This work is also financed by the National Mass Spectrometry Network (RNEM) (POCI-01-0145-FEDER-402-022125 Ref. ROTEIRO/0028/2013). Publisher Copyright: © 2023 by the authors.
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spelling An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera albaAnnelidamarine invertebratesproteomicstoxicitytoxinvenom proteinsToxicologyHealth, Toxicology and MutagenesisSDG 14 - Life Below WaterFunding Information: The Portuguese Foundation for Science and Technology (FCT), i.p., funded project WormALL (PTDC/BTA BTA/28650/2017), which includes the contract attributed to M.D. and the fellowship of A.P.R. Fundo Azul cofinanced project MARVEN (FA_05_2017_007), which includes the fellowship of I.M.C. This work is also financed by the National Mass Spectrometry Network (RNEM) (POCI-01-0145-FEDER-402-022125 Ref. ROTEIRO/0028/2013). Publisher Copyright: © 2023 by the authors.The immense biodiversity of marine invertebrates makes them high-value targets for the prospecting of novel bioactives. The present study investigated proteinaceous toxins secreted by the skin and proboscis of Glycera alba (Annelida: Polychaeta), whose congenerics G. tridactyla and G. dibranchiata are known to be venomous. Proteomics and bioinformatics enabled the detection of bioactive proteins that hold potential for biotechnological applications, including toxins like glycerotoxins (GLTx), which can interfere with neuromuscular calcium channels and therefore have value for the development of painkillers, for instance. We also identified proteins involved in the biosynthesis of toxins. Other proteins of interest include venom and toxin-related bioactives like cysteine-rich venom proteins, many of which are known to interfere with the nervous system. Ex vivo toxicity assays with mussel gills exposed to fractionated protein extracts from the skin and proboscis revealed that fractions potentially containing higher-molecular-mass venom proteins can exert negative effects on invertebrate prey. Histopathology, DNA damage and caspase-3 activity suggest significant cytotoxic effects that can be coadjuvated by permeabilizing enzymes such as venom metalloproteinases M12B. Altogether, these encouraging findings show that venomous annelids are important sources of novel bioactives, albeit illustrating the challenges of surveying organisms whose genomes and metabolisms are poorly understood.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNCampos, SóniaRodrigo, Ana P.Moutinho Cabral, InêsMendes, Vera M.Manadas, BrunoD’Ambrosio, MariaelenaCosta, Pedro M.2024-01-16T22:36:11Z2023-11-142023-11-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://hdl.handle.net/10362/162346eng2072-6651PURE: 81258183https://doi.org/10.3390/toxins15110655info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:45:09Zoai:run.unl.pt:10362/162346Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:51.201621Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba
title An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba
spellingShingle An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba
Campos, Sónia
Annelida
marine invertebrates
proteomics
toxicity
toxin
venom proteins
Toxicology
Health, Toxicology and Mutagenesis
SDG 14 - Life Below Water
title_short An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba
title_full An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba
title_fullStr An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba
title_full_unstemmed An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba
title_sort An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba
author Campos, Sónia
author_facet Campos, Sónia
Rodrigo, Ana P.
Moutinho Cabral, Inês
Mendes, Vera M.
Manadas, Bruno
D’Ambrosio, Mariaelena
Costa, Pedro M.
author_role author
author2 Rodrigo, Ana P.
Moutinho Cabral, Inês
Mendes, Vera M.
Manadas, Bruno
D’Ambrosio, Mariaelena
Costa, Pedro M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Campos, Sónia
Rodrigo, Ana P.
Moutinho Cabral, Inês
Mendes, Vera M.
Manadas, Bruno
D’Ambrosio, Mariaelena
Costa, Pedro M.
dc.subject.por.fl_str_mv Annelida
marine invertebrates
proteomics
toxicity
toxin
venom proteins
Toxicology
Health, Toxicology and Mutagenesis
SDG 14 - Life Below Water
topic Annelida
marine invertebrates
proteomics
toxicity
toxin
venom proteins
Toxicology
Health, Toxicology and Mutagenesis
SDG 14 - Life Below Water
description Funding Information: The Portuguese Foundation for Science and Technology (FCT), i.p., funded project WormALL (PTDC/BTA BTA/28650/2017), which includes the contract attributed to M.D. and the fellowship of A.P.R. Fundo Azul cofinanced project MARVEN (FA_05_2017_007), which includes the fellowship of I.M.C. This work is also financed by the National Mass Spectrometry Network (RNEM) (POCI-01-0145-FEDER-402-022125 Ref. ROTEIRO/0028/2013). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-14
2023-11-14T00:00:00Z
2024-01-16T22:36:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/162346
url http://hdl.handle.net/10362/162346
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2072-6651
PURE: 81258183
https://doi.org/10.3390/toxins15110655
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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