Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/4513 |
Resumo: | This study focused on the use of a new system, an alginate|Ɛ-poly-L-lysine| alginate|chitosan microcapsule (APACM), able to immobilize a folate-producing probiotic, Lactococcus lactis ssp. cremoris (LLC), which provides a new approach to the utilization of capsules and probiotics for in situ production of vitamins. LLC is able to produce 95.25±26 μg·L−1 of folate, during 10 h, andwas encapsulated in the APACM. APACMproved its capacity to protect LLC against the harsh conditions of a simulated digestion maintaining a viable concentration of 6 log CFU·mL−1of LLC. A nutrients exchange capacity test, was performed using Lactobacillus plantarum UM7, a high lactic acid producer was used here to avoid false negative results. The production and release of 2 g·L−1 of lactic acidwas achieved through encapsulation of L. plantarum, after 20 h. The adhesion of APACM to epithelial cells was also quantified, yielding 38% and 33% of capsules adhered to HT-29 cells and Caco-2 cells, respectively. |
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7160 |
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Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validationProbioticImmobilizationMucoadhesiveSegurança AlimentarThis study focused on the use of a new system, an alginate|Ɛ-poly-L-lysine| alginate|chitosan microcapsule (APACM), able to immobilize a folate-producing probiotic, Lactococcus lactis ssp. cremoris (LLC), which provides a new approach to the utilization of capsules and probiotics for in situ production of vitamins. LLC is able to produce 95.25±26 μg·L−1 of folate, during 10 h, andwas encapsulated in the APACM. APACMproved its capacity to protect LLC against the harsh conditions of a simulated digestion maintaining a viable concentration of 6 log CFU·mL−1of LLC. A nutrients exchange capacity test, was performed using Lactobacillus plantarum UM7, a high lactic acid producer was used here to avoid false negative results. The production and release of 2 g·L−1 of lactic acidwas achieved through encapsulation of L. plantarum, after 20 h. The adhesion of APACM to epithelial cells was also quantified, yielding 38% and 33% of capsules adhered to HT-29 cells and Caco-2 cells, respectively.The authors Philippe E. Ramos and Ana Pinheiro, are recipient of fellowships from the Fundacão para a Ciência e Tecnologia, POPHQREN and FSE (FCT, Portugal) through grants, SFRH/BD/80800/ 2012 and SFRH/BPD/101181/2014, respectively. The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the project “BioInd—Biotechnology and Bioengineering for Improved Industrial and Agro-Food Processes”, ref. NORTE-07-0124-FEDER-000028 cofunded by the Programa Operacional Regional do Norte (ON.2–O Novo Norte), QREN, FEDER.Elsevier/Canadian Institute of Food Science and Technology (CIFST)Repositório Científico do Instituto Nacional de SaúdeRamos, Philippe E.Abrunhosa, LuisPinheiro, AnaCerqueira, MiguelMotta, CarlaCastanheira, IsabelChandra-Hioe, Maria V.Arcot, JayashreeTeixeira, José A.Vicente, António A.2016-10-292025-10-24T00:00:00Z2016-10-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4513engFood Res Int. 2016;90:25-32. doi:10.1016/j.foodres.2016.10.0360963-9969http://dx.doi.org/10.1016/j.foodres.2016.10.036info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:18Zoai:repositorio.insa.pt:10400.18/4513Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:09.862082Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation |
title |
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation |
spellingShingle |
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation Ramos, Philippe E. Probiotic Immobilization Mucoadhesive Segurança Alimentar |
title_short |
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation |
title_full |
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation |
title_fullStr |
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation |
title_full_unstemmed |
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation |
title_sort |
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation |
author |
Ramos, Philippe E. |
author_facet |
Ramos, Philippe E. Abrunhosa, Luis Pinheiro, Ana Cerqueira, Miguel Motta, Carla Castanheira, Isabel Chandra-Hioe, Maria V. Arcot, Jayashree Teixeira, José A. Vicente, António A. |
author_role |
author |
author2 |
Abrunhosa, Luis Pinheiro, Ana Cerqueira, Miguel Motta, Carla Castanheira, Isabel Chandra-Hioe, Maria V. Arcot, Jayashree Teixeira, José A. Vicente, António A. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Ramos, Philippe E. Abrunhosa, Luis Pinheiro, Ana Cerqueira, Miguel Motta, Carla Castanheira, Isabel Chandra-Hioe, Maria V. Arcot, Jayashree Teixeira, José A. Vicente, António A. |
dc.subject.por.fl_str_mv |
Probiotic Immobilization Mucoadhesive Segurança Alimentar |
topic |
Probiotic Immobilization Mucoadhesive Segurança Alimentar |
description |
This study focused on the use of a new system, an alginate|Ɛ-poly-L-lysine| alginate|chitosan microcapsule (APACM), able to immobilize a folate-producing probiotic, Lactococcus lactis ssp. cremoris (LLC), which provides a new approach to the utilization of capsules and probiotics for in situ production of vitamins. LLC is able to produce 95.25±26 μg·L−1 of folate, during 10 h, andwas encapsulated in the APACM. APACMproved its capacity to protect LLC against the harsh conditions of a simulated digestion maintaining a viable concentration of 6 log CFU·mL−1of LLC. A nutrients exchange capacity test, was performed using Lactobacillus plantarum UM7, a high lactic acid producer was used here to avoid false negative results. The production and release of 2 g·L−1 of lactic acidwas achieved through encapsulation of L. plantarum, after 20 h. The adhesion of APACM to epithelial cells was also quantified, yielding 38% and 33% of capsules adhered to HT-29 cells and Caco-2 cells, respectively. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-29 2016-10-29T00:00:00Z 2025-10-24T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/4513 |
url |
http://hdl.handle.net/10400.18/4513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Res Int. 2016;90:25-32. doi:10.1016/j.foodres.2016.10.036 0963-9969 http://dx.doi.org/10.1016/j.foodres.2016.10.036 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier/Canadian Institute of Food Science and Technology (CIFST) |
publisher.none.fl_str_mv |
Elsevier/Canadian Institute of Food Science and Technology (CIFST) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132129549877248 |