Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogaster

Detalhes bibliográficos
Autor(a) principal: Leitão, Alexandre Castanho Barata
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/1442
Resumo: Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2009, Universidade de Lisboa, Faculdade de Ciências
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spelling Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogasterImunologiaSistema imunitárioDrosophila melanogasterTeses de mestradoTese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2009, Universidade de Lisboa, Faculdade de CiênciasResumo alargado em português disponível no documentoInsect innate immune system can be partitioned into humoral and cellular defense mechanisms. However, both branches of immune system are interconnected, acting in a synergistic way. The study of these interconnections is an important step to comprehend the immune response as an integrated system. In Drosophila genus 95% of 'blood cells' (hemocytes) are plasmatocytes. These cells participate in numerous processes during development and immune response. Throughout embryogenesis and pupal stage plasmatocytes play an important role in tissue remodeling, phagocytizing dead cells and synthesizing extracellular matrix. During an immune response they are responsible for production of antimicrobial peptides, phagocytosis and aggregation of pathogens. In addition, it is possible to find two other types of hemocytes in Drosophila's hemolymph, crystal cells and lamellocytes, both with very specialized functions in immune response. Crystal cells constitute 5% of hemocytes in Drosophila and lamellocytes are only found in larvae upon infection with large pathogens, such as wasp eggs. Classification of these three hemocyte types is based on morphological and biochemical criteria. However, some new studies have begun to characterize RNA and protein expression in Drosophila's hemocyte classes. Here, we want to go further in the genetic characterization of plasmatocytes with the purpose off asking if there are different subpopulations of plasmatocytes performing different functions during immune response. For this propose we used flow cytometry technique to analyze gene expression in Drosophila larvae plasmatocytes. Our results show that two out of the five GAL4 lines analyzed drive expression of GFP in subpopulations of Drosophila larval plasmatocytes. This observation indicates that plasmatocytes do not form a homogeneous population of cells in Drosophila's larvae hemolymph. We then used Fluorescence Activated Cell Sorting (FACS) to sort and perform in vitro experiments with hemese positive and hemese negative plasmatocytes independently. In vitro analysis confirmed that both subpopulations correspond to previous plasmatocyte descriptions. We hypothesized that one of these two different subpopulations of plasmatocytes is responsible for modulation of melanization, an immune response of insects. However, the results obtained in our specific in vitro setting did not support this hypothesis but further work is needed to ascertain this matter in a definitive way.Sucena, José Élio da SilvaRepositório da Universidade de LisboaLeitão, Alexandre Castanho Barata2010-07-27T08:58:19Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1442enghttp://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000571459info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:40:57Zoai:repositorio.ul.pt:10451/1442Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:28:05.095777Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogaster
title Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogaster
spellingShingle Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogaster
Leitão, Alexandre Castanho Barata
Imunologia
Sistema imunitário
Drosophila melanogaster
Teses de mestrado
title_short Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogaster
title_full Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogaster
title_fullStr Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogaster
title_full_unstemmed Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogaster
title_sort Towards the characterization of plasmatocyte heterogeneity in Drosophila melanogaster
author Leitão, Alexandre Castanho Barata
author_facet Leitão, Alexandre Castanho Barata
author_role author
dc.contributor.none.fl_str_mv Sucena, José Élio da Silva
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Leitão, Alexandre Castanho Barata
dc.subject.por.fl_str_mv Imunologia
Sistema imunitário
Drosophila melanogaster
Teses de mestrado
topic Imunologia
Sistema imunitário
Drosophila melanogaster
Teses de mestrado
description Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2009, Universidade de Lisboa, Faculdade de Ciências
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2010-07-27T08:58:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1442
url http://hdl.handle.net/10451/1442
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000571459
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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