Genome sequencing reveals a phage in Helicobacter pylori

Detalhes bibliográficos
Autor(a) principal: Lehours, Philippe
Data de Publicação: 2011
Outros Autores: Vale, Filipa F., Bjursell, Magnus K., Melefors, Ojar, Advani, Reza, Glavas, Steve, Guegueniat, Julia, Gontier, Etienne, Lacomme, Sabrina, Matos, António Alves, Menard, Armelle, Mégraud, Francis, Engstrand, Lars, Andersson, Anders F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/42051
Resumo: Helicobacter pylori chronically infects the gastric mucosa in more than half of the human population; in a subset of this population, its presence is associated with development of severe disease, such as gastric cancer. Genomic analysis of several strains has revealed an extensive H pylori pan-genome, likely to grow as more genomes are sampled. Here we describe the draft genome sequence (63 contigs; 26× mean coverage) of H pylori strain B45, isolated from a patient with gastric mucosa-associated lymphoid tissue (MALT) lymphoma. The major finding was a 24.6-kb prophage integrated in the bacterial genome. The prophage shares most of its genes (22/27) with prophage region II of Helicobacter acinonychis strain Sheeba. After UV treatment of liquid cultures, circular DNA carrying the prophage integrase gene could be detected, and intracellular tailed phage-like particles were observed in H pylori cells by transmission electron microscopy, indicating that phage production can be induced from the prophage. PCR amplification and sequencing of the integrase gene from 341 H pylori strains from different geographic regions revealed a high prevalence of the prophage (21.4%). Phylogenetic reconstruction showed four distinct clusters in the integrase gene, three of which tended to be specific for geographic regions. Our study implies that phages may play important roles in the ecology and evolution of H pylori.
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spelling Genome sequencing reveals a phage in Helicobacter pyloriHelicobacter pylori chronically infects the gastric mucosa in more than half of the human population; in a subset of this population, its presence is associated with development of severe disease, such as gastric cancer. Genomic analysis of several strains has revealed an extensive H pylori pan-genome, likely to grow as more genomes are sampled. Here we describe the draft genome sequence (63 contigs; 26× mean coverage) of H pylori strain B45, isolated from a patient with gastric mucosa-associated lymphoid tissue (MALT) lymphoma. The major finding was a 24.6-kb prophage integrated in the bacterial genome. The prophage shares most of its genes (22/27) with prophage region II of Helicobacter acinonychis strain Sheeba. After UV treatment of liquid cultures, circular DNA carrying the prophage integrase gene could be detected, and intracellular tailed phage-like particles were observed in H pylori cells by transmission electron microscopy, indicating that phage production can be induced from the prophage. PCR amplification and sequencing of the integrase gene from 341 H pylori strains from different geographic regions revealed a high prevalence of the prophage (21.4%). Phylogenetic reconstruction showed four distinct clusters in the integrase gene, three of which tended to be specific for geographic regions. Our study implies that phages may play important roles in the ecology and evolution of H pylori.Veritati - Repositório Institucional da Universidade Católica PortuguesaLehours, PhilippeVale, Filipa F.Bjursell, Magnus K.Melefors, OjarAdvani, RezaGlavas, SteveGuegueniat, JuliaGontier, EtienneLacomme, SabrinaMatos, António AlvesMenard, ArmelleMégraud, FrancisEngstrand, LarsAndersson, Anders F.2023-08-09T08:36:27Z2011-11-152011-11-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/42051eng2161-212910.1128/mBio.00239-118485523405522086490000299992700013info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-15T01:42:54Zoai:repositorio.ucp.pt:10400.14/42051Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:27:02.476653Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genome sequencing reveals a phage in Helicobacter pylori
title Genome sequencing reveals a phage in Helicobacter pylori
spellingShingle Genome sequencing reveals a phage in Helicobacter pylori
Lehours, Philippe
title_short Genome sequencing reveals a phage in Helicobacter pylori
title_full Genome sequencing reveals a phage in Helicobacter pylori
title_fullStr Genome sequencing reveals a phage in Helicobacter pylori
title_full_unstemmed Genome sequencing reveals a phage in Helicobacter pylori
title_sort Genome sequencing reveals a phage in Helicobacter pylori
author Lehours, Philippe
author_facet Lehours, Philippe
Vale, Filipa F.
Bjursell, Magnus K.
Melefors, Ojar
Advani, Reza
Glavas, Steve
Guegueniat, Julia
Gontier, Etienne
Lacomme, Sabrina
Matos, António Alves
Menard, Armelle
Mégraud, Francis
Engstrand, Lars
Andersson, Anders F.
author_role author
author2 Vale, Filipa F.
Bjursell, Magnus K.
Melefors, Ojar
Advani, Reza
Glavas, Steve
Guegueniat, Julia
Gontier, Etienne
Lacomme, Sabrina
Matos, António Alves
Menard, Armelle
Mégraud, Francis
Engstrand, Lars
Andersson, Anders F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Lehours, Philippe
Vale, Filipa F.
Bjursell, Magnus K.
Melefors, Ojar
Advani, Reza
Glavas, Steve
Guegueniat, Julia
Gontier, Etienne
Lacomme, Sabrina
Matos, António Alves
Menard, Armelle
Mégraud, Francis
Engstrand, Lars
Andersson, Anders F.
description Helicobacter pylori chronically infects the gastric mucosa in more than half of the human population; in a subset of this population, its presence is associated with development of severe disease, such as gastric cancer. Genomic analysis of several strains has revealed an extensive H pylori pan-genome, likely to grow as more genomes are sampled. Here we describe the draft genome sequence (63 contigs; 26× mean coverage) of H pylori strain B45, isolated from a patient with gastric mucosa-associated lymphoid tissue (MALT) lymphoma. The major finding was a 24.6-kb prophage integrated in the bacterial genome. The prophage shares most of its genes (22/27) with prophage region II of Helicobacter acinonychis strain Sheeba. After UV treatment of liquid cultures, circular DNA carrying the prophage integrase gene could be detected, and intracellular tailed phage-like particles were observed in H pylori cells by transmission electron microscopy, indicating that phage production can be induced from the prophage. PCR amplification and sequencing of the integrase gene from 341 H pylori strains from different geographic regions revealed a high prevalence of the prophage (21.4%). Phylogenetic reconstruction showed four distinct clusters in the integrase gene, three of which tended to be specific for geographic regions. Our study implies that phages may play important roles in the ecology and evolution of H pylori.
publishDate 2011
dc.date.none.fl_str_mv 2011-11-15
2011-11-15T00:00:00Z
2023-08-09T08:36:27Z
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