Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/37716 |
Resumo: | Cheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, β-lactoglobulin and α-lactalbumin, key components of cheese whey. ATPS formed by PEG (molecular weights: 200–8000 g.mol−1) with sodium or ammonium sulfate were investigated. Partitioning of the selected solutes was experimentally addressed in different ATPS and pH values. Partition behavior showed that ATPS formed by PEG1500/ammonium sulfate is able to separate lactose from proteins, while PEG300/sodium sulfate ATPS may be used for protein fractionation. These separation strategies were then tested with simulated and real cheese whey. Under optimized conditions, PEG 1500/ammonium sulfate ATPS allows efficient recovery of > 95% proteins (precipitate) and 80% of lactose (bottom phase), as confirmed for both simulated and real cheese whey. The results found indicate that the proposed polymer/salt ATPS can be used to design scalable and cost-effective separation strategies to apply in cheese whey and other related wastes. |
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Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systemsAqueous two-phase systemsCheese wheyBovine serum albuminβ-lactoglobulinα-lactalbuminSeparationCheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, β-lactoglobulin and α-lactalbumin, key components of cheese whey. ATPS formed by PEG (molecular weights: 200–8000 g.mol−1) with sodium or ammonium sulfate were investigated. Partitioning of the selected solutes was experimentally addressed in different ATPS and pH values. Partition behavior showed that ATPS formed by PEG1500/ammonium sulfate is able to separate lactose from proteins, while PEG300/sodium sulfate ATPS may be used for protein fractionation. These separation strategies were then tested with simulated and real cheese whey. Under optimized conditions, PEG 1500/ammonium sulfate ATPS allows efficient recovery of > 95% proteins (precipitate) and 80% of lactose (bottom phase), as confirmed for both simulated and real cheese whey. The results found indicate that the proposed polymer/salt ATPS can be used to design scalable and cost-effective separation strategies to apply in cheese whey and other related wastes.Elsevier2023-05-15T11:24:00Z2021-01-15T00:00:00Z2021-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37716eng1383-586610.1016/j.seppur.2020.117686González Amado, MarlenTavares, Ana P.M.Freire, Mara G.Soto, AnaRodríguez, Oscarinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:13:41Zoai:ria.ua.pt:10773/37716Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:08:19.227557Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems |
title |
Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems |
spellingShingle |
Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems González Amado, Marlen Aqueous two-phase systems Cheese whey Bovine serum albumin β-lactoglobulin α-lactalbumin Separation |
title_short |
Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems |
title_full |
Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems |
title_fullStr |
Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems |
title_full_unstemmed |
Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems |
title_sort |
Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems |
author |
González Amado, Marlen |
author_facet |
González Amado, Marlen Tavares, Ana P.M. Freire, Mara G. Soto, Ana Rodríguez, Oscar |
author_role |
author |
author2 |
Tavares, Ana P.M. Freire, Mara G. Soto, Ana Rodríguez, Oscar |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
González Amado, Marlen Tavares, Ana P.M. Freire, Mara G. Soto, Ana Rodríguez, Oscar |
dc.subject.por.fl_str_mv |
Aqueous two-phase systems Cheese whey Bovine serum albumin β-lactoglobulin α-lactalbumin Separation |
topic |
Aqueous two-phase systems Cheese whey Bovine serum albumin β-lactoglobulin α-lactalbumin Separation |
description |
Cheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, β-lactoglobulin and α-lactalbumin, key components of cheese whey. ATPS formed by PEG (molecular weights: 200–8000 g.mol−1) with sodium or ammonium sulfate were investigated. Partitioning of the selected solutes was experimentally addressed in different ATPS and pH values. Partition behavior showed that ATPS formed by PEG1500/ammonium sulfate is able to separate lactose from proteins, while PEG300/sodium sulfate ATPS may be used for protein fractionation. These separation strategies were then tested with simulated and real cheese whey. Under optimized conditions, PEG 1500/ammonium sulfate ATPS allows efficient recovery of > 95% proteins (precipitate) and 80% of lactose (bottom phase), as confirmed for both simulated and real cheese whey. The results found indicate that the proposed polymer/salt ATPS can be used to design scalable and cost-effective separation strategies to apply in cheese whey and other related wastes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-15T00:00:00Z 2021-01-15 2023-05-15T11:24:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/37716 |
url |
http://hdl.handle.net/10773/37716 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1383-5866 10.1016/j.seppur.2020.117686 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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