Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin

Detalhes bibliográficos
Autor(a) principal: Freire, Luziany Adyja da Costa
Data de Publicação: 2019
Outros Autores: Courtial, Camila Gambini Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32360
Resumo: The recovery of biomolecules represents an important field of study in evolution with fundamental application in the advances of biotechnology. In this work, the study of α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) partitioning was carried out using aqueous two-phase systems (formed by polymer/maltodextrin) with different synthetic polymers (polyethylene glycol_PEG, polypropylene glycol_PPG, polyvinylpyrrolidone_PVP) and different molecular weights (from 425 to 40000). The influence of the type, molecular weight and percentage of the polymer used in the system was evaluated in the partition of these proteins. The results indicated that maltodextrin may be a good substitute for dextran in the processes with aqueous two phase aqueous system and it was observed that after the process the proteins tend to remain in the maltodextrin rich phase
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spelling Freire, Luziany Adyja da CostaCourtial, Camila Gambini Pereira2021-05-04T13:53:54Z2021-05-04T13:53:54Z2019-07-01FREIRE, L. A. C.; PEREIRA, C.G.. Evaluation of α-lactoalbumin and β-lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer / maltodextrin. Journal of Environment and Biotechnology Research, v. 8, p. 29-37, 2019. Disponível em: https://zenodo.org/record/3263512#.YGNyd69KiM9. Acesso em: 30 mar. 2021. https://doi.org/10.5281/zenodo.3263512https://repositorio.ufrn.br/handle/123456789/3236010.5281/zenodo.3263512Vinanie PublishersAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessα-lactalbumin (α-La)β-lactoglobulin (β-Lg)Aqueous two-phase systemSeparationEvaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe recovery of biomolecules represents an important field of study in evolution with fundamental application in the advances of biotechnology. In this work, the study of α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) partitioning was carried out using aqueous two-phase systems (formed by polymer/maltodextrin) with different synthetic polymers (polyethylene glycol_PEG, polypropylene glycol_PPG, polyvinylpyrrolidone_PVP) and different molecular weights (from 425 to 40000). The influence of the type, molecular weight and percentage of the polymer used in the system was evaluated in the partition of these proteins. The results indicated that maltodextrin may be a good substitute for dextran in the processes with aqueous two phase aqueous system and it was observed that after the process the proteins tend to remain in the maltodextrin rich phaseengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALEvaluationOfα-LactoalbuminAndβ-L_PEREIRA_2019.pdfEvaluationOfα-LactoalbuminAndβ-L_PEREIRA_2019.pdfArtigoapplication/pdf377159https://repositorio.ufrn.br/bitstream/123456789/32360/1/EvaluationOf%ce%b1-LactoalbuminAnd%ce%b2-L_PEREIRA_2019.pdf90b4e144662b4238078abcfb3b012b0aMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32360/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32360/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/323602021-05-04 10:53:55.311oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-05-04T13:53:55Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin
title Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin
spellingShingle Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin
Freire, Luziany Adyja da Costa
α-lactalbumin (α-La)
β-lactoglobulin (β-Lg)
Aqueous two-phase system
Separation
title_short Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin
title_full Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin
title_fullStr Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin
title_full_unstemmed Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin
title_sort Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin
author Freire, Luziany Adyja da Costa
author_facet Freire, Luziany Adyja da Costa
Courtial, Camila Gambini Pereira
author_role author
author2 Courtial, Camila Gambini Pereira
author2_role author
dc.contributor.author.fl_str_mv Freire, Luziany Adyja da Costa
Courtial, Camila Gambini Pereira
dc.subject.por.fl_str_mv α-lactalbumin (α-La)
β-lactoglobulin (β-Lg)
Aqueous two-phase system
Separation
topic α-lactalbumin (α-La)
β-lactoglobulin (β-Lg)
Aqueous two-phase system
Separation
description The recovery of biomolecules represents an important field of study in evolution with fundamental application in the advances of biotechnology. In this work, the study of α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) partitioning was carried out using aqueous two-phase systems (formed by polymer/maltodextrin) with different synthetic polymers (polyethylene glycol_PEG, polypropylene glycol_PPG, polyvinylpyrrolidone_PVP) and different molecular weights (from 425 to 40000). The influence of the type, molecular weight and percentage of the polymer used in the system was evaluated in the partition of these proteins. The results indicated that maltodextrin may be a good substitute for dextran in the processes with aqueous two phase aqueous system and it was observed that after the process the proteins tend to remain in the maltodextrin rich phase
publishDate 2019
dc.date.issued.fl_str_mv 2019-07-01
dc.date.accessioned.fl_str_mv 2021-05-04T13:53:54Z
dc.date.available.fl_str_mv 2021-05-04T13:53:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FREIRE, L. A. C.; PEREIRA, C.G.. Evaluation of α-lactoalbumin and β-lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer / maltodextrin. Journal of Environment and Biotechnology Research, v. 8, p. 29-37, 2019. Disponível em: https://zenodo.org/record/3263512#.YGNyd69KiM9. Acesso em: 30 mar. 2021. https://doi.org/10.5281/zenodo.3263512
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32360
dc.identifier.doi.none.fl_str_mv 10.5281/zenodo.3263512
identifier_str_mv FREIRE, L. A. C.; PEREIRA, C.G.. Evaluation of α-lactoalbumin and β-lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer / maltodextrin. Journal of Environment and Biotechnology Research, v. 8, p. 29-37, 2019. Disponível em: https://zenodo.org/record/3263512#.YGNyd69KiM9. Acesso em: 30 mar. 2021. https://doi.org/10.5281/zenodo.3263512
10.5281/zenodo.3263512
url https://repositorio.ufrn.br/handle/123456789/32360
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Vinanie Publishers
publisher.none.fl_str_mv Vinanie Publishers
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
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