Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/10763 |
Resumo: | © 2012 Bentham Science Publishers |
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7160 |
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Antimicrobial peptide rBPI21: a translational overview from bench to clinical studiesrBPI21LipopolysaccharideMicrobicideMembranesClinical trials© 2012 Bentham Science PublishersGram-negative bacteria infection is sometimes followed by septic shock. This serious health condition is caused by the segregation of the lipopolysaccharide (LPS) from bacterial membrane into the bloodstream. Due to bacterial resistance, new antibiotics are needed. Most of the active antibiotics possess bactericidal effect, but lack LPS neutralization properties to prevent or neutralize septic shock. Antimicrobial peptides are a new class of antibiotics not prone to bacterial resistance, because their main target is the membrane. It is difficult for bacteria to critically change their membrane composition without affecting its molecular processes. rBPI21 is a recombinant antimicrobial peptide developed from an antimicrobial protein produced in neutrophils, the bactericidal/permeability-increasing protein (BPI) that ended phase III clinical trials against meningitis with success, reducing serious complications, such as amputations. It interacts preferentially with LPS with high affinity and at the same time has bactericidal effect. Here, we gather evidence that the interaction of the rBPI21 with LPS is mainly electrostatic, first, followed by massive LPS aggregation, which is correlated with its clearance from the bloodstream. The molecular mechanism at membrane level includes the peptide interactions with negatively charged phospholipids that promote outer and inner membrane hemi(fusion). This perturbation is followed by membrane permeabilization.This work was partially funded by Fundação para a Ciência e a Tecnologia of the Ministério da Educação Ciência, Tecnologia e Ensino Superior (FCT-MEC, Portugal). MMD also acknowledges FCT-MEC for fellowship SFRH/BD/41750/2007.Bentham Science PublishersRepositório da Universidade de LisboaDomingues, Marco M.Santos, Nuno C.Castanho, Miguel A. R. B.2014-03-21T11:06:03Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10763engCurrent Protein and Peptide Science, 2012, 13, 611-6191389-2037http://dx.doi.org/10.2174/138920312804142101metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:14:43Zoai:repositorio.ul.pt:10451/10763Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:14:43Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies |
title |
Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies |
spellingShingle |
Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies Domingues, Marco M. rBPI21 Lipopolysaccharide Microbicide Membranes Clinical trials |
title_short |
Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies |
title_full |
Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies |
title_fullStr |
Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies |
title_full_unstemmed |
Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies |
title_sort |
Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies |
author |
Domingues, Marco M. |
author_facet |
Domingues, Marco M. Santos, Nuno C. Castanho, Miguel A. R. B. |
author_role |
author |
author2 |
Santos, Nuno C. Castanho, Miguel A. R. B. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Domingues, Marco M. Santos, Nuno C. Castanho, Miguel A. R. B. |
dc.subject.por.fl_str_mv |
rBPI21 Lipopolysaccharide Microbicide Membranes Clinical trials |
topic |
rBPI21 Lipopolysaccharide Microbicide Membranes Clinical trials |
description |
© 2012 Bentham Science Publishers |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z 2014-03-21T11:06:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/10763 |
url |
http://hdl.handle.net/10451/10763 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Current Protein and Peptide Science, 2012, 13, 611-619 1389-2037 http://dx.doi.org/10.2174/138920312804142101 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Bentham Science Publishers |
publisher.none.fl_str_mv |
Bentham Science Publishers |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817548809959374848 |