Pulsed electric field reduces the permeability of potato cell wall

Detalhes bibliográficos
Autor(a) principal: Gómez Galindo, Federico
Data de Publicação: 2008
Outros Autores: Vernier, P. Thomas, Dejmek, Petr, Vicente, A. A., Gundersen, Martin A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/10424
Resumo: The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.
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spelling Pulsed electric field reduces the permeability of potato cell wallCell wallFM1-43eEectric fieldsSressOxidative cross-linkingelectric fieldsstressScience & TechnologyThe effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.John Wiley and SonsUniversidade do MinhoGómez Galindo, FedericoVernier, P. ThomasDejmek, PetrVicente, A. A.Gundersen, Martin A.2008-052008-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/10424eng"Bioelectromagnetic". ISSN 0197-8462. 29:4 (May 2008) 296-301.0197-846210.1002/bem.2039418163439info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:30Zoai:repositorium.sdum.uminho.pt:1822/10424Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:48.976041Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Pulsed electric field reduces the permeability of potato cell wall
title Pulsed electric field reduces the permeability of potato cell wall
spellingShingle Pulsed electric field reduces the permeability of potato cell wall
Gómez Galindo, Federico
Cell wall
FM1-43
eEectric fields
Sress
Oxidative cross-linking
electric fields
stress
Science & Technology
title_short Pulsed electric field reduces the permeability of potato cell wall
title_full Pulsed electric field reduces the permeability of potato cell wall
title_fullStr Pulsed electric field reduces the permeability of potato cell wall
title_full_unstemmed Pulsed electric field reduces the permeability of potato cell wall
title_sort Pulsed electric field reduces the permeability of potato cell wall
author Gómez Galindo, Federico
author_facet Gómez Galindo, Federico
Vernier, P. Thomas
Dejmek, Petr
Vicente, A. A.
Gundersen, Martin A.
author_role author
author2 Vernier, P. Thomas
Dejmek, Petr
Vicente, A. A.
Gundersen, Martin A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gómez Galindo, Federico
Vernier, P. Thomas
Dejmek, Petr
Vicente, A. A.
Gundersen, Martin A.
dc.subject.por.fl_str_mv Cell wall
FM1-43
eEectric fields
Sress
Oxidative cross-linking
electric fields
stress
Science & Technology
topic Cell wall
FM1-43
eEectric fields
Sress
Oxidative cross-linking
electric fields
stress
Science & Technology
description The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.
publishDate 2008
dc.date.none.fl_str_mv 2008-05
2008-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/10424
url http://hdl.handle.net/1822/10424
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Bioelectromagnetic". ISSN 0197-8462. 29:4 (May 2008) 296-301.
0197-8462
10.1002/bem.20394
18163439
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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