Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.

Detalhes bibliográficos
Autor(a) principal: Vilhena, Maria Manuela Clemente
Data de Publicação: 2014
Outros Autores: Milhano, Natacha, Popov, Vsevolod, Jost, Cristine, de Sousa, Rita, Walker, David H., Bouyer, Donald H.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/13837
Resumo: Rickettsia massiliae, belonging to the spotted fever group of Rickettsia, is a human pathogen causing a similar course of disease to that caused by R. conorii, the originally recognized etiologic agent of Mediterranean spotted fever. In view of this similarity, we performed an ultrastructural study of R. massiliae in organs of Rhipicephalus sanguineus ticks, in order to advance knowledge of the complex dynamics at the tick-pathogen interface in rickettsioses. Adult R. massiliae-infected Rh. sanguineus ticks were fed on uninfected Hartley strain guinea pigs, and five females were collected daily throughout their feeding period up to day 6, and analyzed by quantitative real-time PCR and electron microscopy. An increase in rickettsial content was observed in the salivary glands, particularly in the first two days of feeding, and a plateau was observed between days 3 and 6. Rickettsial organisms were observed in all tick organs analyzed, in higher numbers in the fed state, and statistically significant differences were observed in measurements of the periplasmic layer of R. massiliae in salivary glands of fed and unfed Rh. sanguineus ticks, with increased thickness in the former case. This study provides insight into the interface between R. massiliae and Rh. sanguineus ticks, highlighting the need for analysis of R. massiliae to fully ascertain its place as an important pathogenic agent of a spotted fever rickettsiosis.
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spelling Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.PARASITOLOGYTICKSRickettsia massiliae, belonging to the spotted fever group of Rickettsia, is a human pathogen causing a similar course of disease to that caused by R. conorii, the originally recognized etiologic agent of Mediterranean spotted fever. In view of this similarity, we performed an ultrastructural study of R. massiliae in organs of Rhipicephalus sanguineus ticks, in order to advance knowledge of the complex dynamics at the tick-pathogen interface in rickettsioses. Adult R. massiliae-infected Rh. sanguineus ticks were fed on uninfected Hartley strain guinea pigs, and five females were collected daily throughout their feeding period up to day 6, and analyzed by quantitative real-time PCR and electron microscopy. An increase in rickettsial content was observed in the salivary glands, particularly in the first two days of feeding, and a plateau was observed between days 3 and 6. Rickettsial organisms were observed in all tick organs analyzed, in higher numbers in the fed state, and statistically significant differences were observed in measurements of the periplasmic layer of R. massiliae in salivary glands of fed and unfed Rh. sanguineus ticks, with increased thickness in the former case. This study provides insight into the interface between R. massiliae and Rh. sanguineus ticks, highlighting the need for analysis of R. massiliae to fully ascertain its place as an important pathogenic agent of a spotted fever rickettsiosis.Ticks and Tick-borne Diseases 5 (2014) 709–7142015-03-30T18:04:22Z2015-03-302014-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/13837http://hdl.handle.net/10174/13837porMilhano, Natacha; Popov, Vsevolod;de Sousa, Rita; Walker, David H.;Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs. 5 pp., www.elsevier.com/locate/ttbdis: Ticks and Tick-borne Diseases 5 (2014) 709–714, 2014.1877-959XISSN: 1877-959Xndndndndndndnd384Vilhena, Maria Manuela ClementeMilhano, NatachaPopov, VsevolodJost, Cristinede Sousa, RitaWalker, David H.Bouyer, Donald H.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:59:09Zoai:dspace.uevora.pt:10174/13837Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:07:00.531349Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.
title Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.
spellingShingle Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.
Vilhena, Maria Manuela Clemente
PARASITOLOGY
TICKS
title_short Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.
title_full Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.
title_fullStr Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.
title_full_unstemmed Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.
title_sort Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.
author Vilhena, Maria Manuela Clemente
author_facet Vilhena, Maria Manuela Clemente
Milhano, Natacha
Popov, Vsevolod
Jost, Cristine
de Sousa, Rita
Walker, David H.
Bouyer, Donald H.
author_role author
author2 Milhano, Natacha
Popov, Vsevolod
Jost, Cristine
de Sousa, Rita
Walker, David H.
Bouyer, Donald H.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vilhena, Maria Manuela Clemente
Milhano, Natacha
Popov, Vsevolod
Jost, Cristine
de Sousa, Rita
Walker, David H.
Bouyer, Donald H.
dc.subject.por.fl_str_mv PARASITOLOGY
TICKS
topic PARASITOLOGY
TICKS
description Rickettsia massiliae, belonging to the spotted fever group of Rickettsia, is a human pathogen causing a similar course of disease to that caused by R. conorii, the originally recognized etiologic agent of Mediterranean spotted fever. In view of this similarity, we performed an ultrastructural study of R. massiliae in organs of Rhipicephalus sanguineus ticks, in order to advance knowledge of the complex dynamics at the tick-pathogen interface in rickettsioses. Adult R. massiliae-infected Rh. sanguineus ticks were fed on uninfected Hartley strain guinea pigs, and five females were collected daily throughout their feeding period up to day 6, and analyzed by quantitative real-time PCR and electron microscopy. An increase in rickettsial content was observed in the salivary glands, particularly in the first two days of feeding, and a plateau was observed between days 3 and 6. Rickettsial organisms were observed in all tick organs analyzed, in higher numbers in the fed state, and statistically significant differences were observed in measurements of the periplasmic layer of R. massiliae in salivary glands of fed and unfed Rh. sanguineus ticks, with increased thickness in the former case. This study provides insight into the interface between R. massiliae and Rh. sanguineus ticks, highlighting the need for analysis of R. massiliae to fully ascertain its place as an important pathogenic agent of a spotted fever rickettsiosis.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-01T00:00:00Z
2015-03-30T18:04:22Z
2015-03-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/13837
http://hdl.handle.net/10174/13837
url http://hdl.handle.net/10174/13837
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Milhano, Natacha; Popov, Vsevolod;de Sousa, Rita; Walker, David H.;Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs. 5 pp., www.elsevier.com/locate/ttbdis: Ticks and Tick-borne Diseases 5 (2014) 709–714, 2014.
1877-959X
ISSN: 1877-959X
nd
nd
nd
nd
nd
nd
nd
384
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Ticks and Tick-borne Diseases 5 (2014) 709–714
publisher.none.fl_str_mv Ticks and Tick-borne Diseases 5 (2014) 709–714
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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