Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/24375 |
Resumo: | Chestnut blight, Cryphonectria parasitica (Murrill) Barr, was reported in Portugal in 1989. The disease rapidly spread within the chestnut region of the Trás-os-Montes (the Northeast of Portugal). Eradication and mechanical/burning exclusion failed to control the disease and natural hypovirulence is still absent or of very low incidence. The introduction of human-assisted therapeutic hypovirulence is therefore required to control the disease. We presente here the efficacy of field applications (short and long term) of a locally characterized hypovirulent strain (CHV1-RBB111), by the punch method in two chestnut orchards (Valpaços and Vinhais). The Valpaços disease incidence was 68% (65 out of 96 trees) and in Vinhais it was 46% (78 out of 169 trees). In both locations, diversity of vegetative incompatibility (vc type) was low and largely dominated (> 85%) by a single vc type (EU11). In Valpaços, all cankers of the orchard were treated (63 cankers) and in Vinhais 31 trees (cankers) were treated. One year after application, field efficacy was very high with 93.8% of healed cankers in Valpaços and 90.3% in Vinhais, these values increased to 100% and 93.5% (respectively) after four years. The persistence of the introduced CHV1-RBB 111 strain was high and present in almost all treated cankers and it was dominant both inwards and on the application site revealing an endophytic behavior. The non-C. parasitica microfungi were abundant with 14 different species. Biscogniauxia mediterranea (De Not.) Kuntze was the most abundant in both orchards. Treatment with the introduced hypovirulent strain (CHV1-RBB111) was found to be effective in controlling chestnut blight with regeneration of cortical tissues and recovery of treated trees. |
id |
RCAP_ec7c2b0768af2f1c6126057564e11182 |
---|---|
oai_identifier_str |
oai:bibliotecadigital.ipb.pt:10198/24375 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blightChestnut blightCryphonectria parasitica (Murr.) BarrHuman-assisted therapeutic hypovirulenceHypovirulent strain CHV1-RBB111Chestnut blight, Cryphonectria parasitica (Murrill) Barr, was reported in Portugal in 1989. The disease rapidly spread within the chestnut region of the Trás-os-Montes (the Northeast of Portugal). Eradication and mechanical/burning exclusion failed to control the disease and natural hypovirulence is still absent or of very low incidence. The introduction of human-assisted therapeutic hypovirulence is therefore required to control the disease. We presente here the efficacy of field applications (short and long term) of a locally characterized hypovirulent strain (CHV1-RBB111), by the punch method in two chestnut orchards (Valpaços and Vinhais). The Valpaços disease incidence was 68% (65 out of 96 trees) and in Vinhais it was 46% (78 out of 169 trees). In both locations, diversity of vegetative incompatibility (vc type) was low and largely dominated (> 85%) by a single vc type (EU11). In Valpaços, all cankers of the orchard were treated (63 cankers) and in Vinhais 31 trees (cankers) were treated. One year after application, field efficacy was very high with 93.8% of healed cankers in Valpaços and 90.3% in Vinhais, these values increased to 100% and 93.5% (respectively) after four years. The persistence of the introduced CHV1-RBB 111 strain was high and present in almost all treated cankers and it was dominant both inwards and on the application site revealing an endophytic behavior. The non-C. parasitica microfungi were abundant with 14 different species. Biscogniauxia mediterranea (De Not.) Kuntze was the most abundant in both orchards. Treatment with the introduced hypovirulent strain (CHV1-RBB111) was found to be effective in controlling chestnut blight with regeneration of cortical tissues and recovery of treated trees.The authors are grateful to the Project BioChestnut -IPM- Implement effective control strategies against the chestnut and almond diseases under the Programme PDR2020- 101-030959 for financial support. This study was funded by the Project BioChestnut- IPM (PDR2020–101-030959).Data availabilityThe datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.Biblioteca Digital do IPBCoelho, ValentimNunes, LuísGouveia, Maria Eugénia2018-01-19T10:00:00Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/24375engCoelho, Valentim; Nunes, Luís; Gouveia, Eugénia (2021). Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight. European Journal of Plant Pathology. ISSN 0929-1873. 159:4, p. 769-7810929-187310.1007/s10658-021-02200-3info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:54:32Zoai:bibliotecadigital.ipb.pt:10198/24375Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:15:13.240199Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight |
title |
Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight |
spellingShingle |
Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight Coelho, Valentim Chestnut blight Cryphonectria parasitica (Murr.) Barr Human-assisted therapeutic hypovirulence Hypovirulent strain CHV1-RBB111 |
title_short |
Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight |
title_full |
Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight |
title_fullStr |
Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight |
title_full_unstemmed |
Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight |
title_sort |
Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight |
author |
Coelho, Valentim |
author_facet |
Coelho, Valentim Nunes, Luís Gouveia, Maria Eugénia |
author_role |
author |
author2 |
Nunes, Luís Gouveia, Maria Eugénia |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Coelho, Valentim Nunes, Luís Gouveia, Maria Eugénia |
dc.subject.por.fl_str_mv |
Chestnut blight Cryphonectria parasitica (Murr.) Barr Human-assisted therapeutic hypovirulence Hypovirulent strain CHV1-RBB111 |
topic |
Chestnut blight Cryphonectria parasitica (Murr.) Barr Human-assisted therapeutic hypovirulence Hypovirulent strain CHV1-RBB111 |
description |
Chestnut blight, Cryphonectria parasitica (Murrill) Barr, was reported in Portugal in 1989. The disease rapidly spread within the chestnut region of the Trás-os-Montes (the Northeast of Portugal). Eradication and mechanical/burning exclusion failed to control the disease and natural hypovirulence is still absent or of very low incidence. The introduction of human-assisted therapeutic hypovirulence is therefore required to control the disease. We presente here the efficacy of field applications (short and long term) of a locally characterized hypovirulent strain (CHV1-RBB111), by the punch method in two chestnut orchards (Valpaços and Vinhais). The Valpaços disease incidence was 68% (65 out of 96 trees) and in Vinhais it was 46% (78 out of 169 trees). In both locations, diversity of vegetative incompatibility (vc type) was low and largely dominated (> 85%) by a single vc type (EU11). In Valpaços, all cankers of the orchard were treated (63 cankers) and in Vinhais 31 trees (cankers) were treated. One year after application, field efficacy was very high with 93.8% of healed cankers in Valpaços and 90.3% in Vinhais, these values increased to 100% and 93.5% (respectively) after four years. The persistence of the introduced CHV1-RBB 111 strain was high and present in almost all treated cankers and it was dominant both inwards and on the application site revealing an endophytic behavior. The non-C. parasitica microfungi were abundant with 14 different species. Biscogniauxia mediterranea (De Not.) Kuntze was the most abundant in both orchards. Treatment with the introduced hypovirulent strain (CHV1-RBB111) was found to be effective in controlling chestnut blight with regeneration of cortical tissues and recovery of treated trees. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/24375 |
url |
http://hdl.handle.net/10198/24375 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Coelho, Valentim; Nunes, Luís; Gouveia, Eugénia (2021). Short and long term efficacy and prevalence of Cryphonectria parasitica hypovirulent strains released as biocontrol agents of chestnut blight. European Journal of Plant Pathology. ISSN 0929-1873. 159:4, p. 769-781 0929-1873 10.1007/s10658-021-02200-3 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799135433997680640 |