Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2020
Outros Autores: Twarużek, Magdalena, Dias, Marta, Almeida, Beatriz, Carolino, Elisabete, Soszczyńska, Ewelina, Ałtyn, Iwona, Viegas, Susana, Caetano, Liliana Aranha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/12198
Resumo: Project nº 23222 (02/SAICT/2016).
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spelling Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?Occupational healthOccupational exposureFiltering respiratory protective devicesWaste sorting industryIn vitro modelsCytotoxic potentialRisk characterizationRisk managementProject nº 23222 (02/SAICT/2016)Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”Project IPL/2018/WasteFRPD_ESTeSLProject nº 23222 (02/SAICT/2016).Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL).The use of Filtering Respiratory Protective Devices (FRPD) is mandatory in Portugal to protect workers from the waste industry of harmful exposures. Deleterious health effects of exposure to bioburden via inhalation and/or ingestion include respiratory symptoms and nephrotoxicity. Between January and February 2019, 118 FRPD samples were collected in one waste sorting industry and characterized regarding microbial contamination and cytotoxicity, defined as cell metabolic activity, through the MTT colorimetric assay (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide). Cytotoxic effect was classified according to the percentage of extinction values with respect to the control group, as follows: absent (≥90); low (80% to 90%, +); medium (60% to 79%, ++); and high (below 60%, +++). For 113 samples the MTT assay revealed a cytotoxic effect in A549 cells, of which 81 presented high cytotoxicity. In SK cells, a cytotoxic effect was observed in 56 samples, of which five displayed a high cytotoxic effect. Several moderate (p<0.05) to strong (p<0.01) correlations were found between higher bacterial and fungal counts both in interior layers (fungi and bacteria) and in exhalation valves (fungi) of FRPD samples and reduced cellular metabolic activity of SK cells. On the basis of the obtained results for the cytotoxic effect of FRPD samples on two different cells lines, it was determined that A549 cells exhibited a cytotoxic effect for a higher number of FRPD, whereas the SK cells model correlated better with the other assessed parameters, namely, bacterial and fungal load and conditions of FRPD use. Although the results are not conclusive on the most appropriate cell line to assess FRPD cytotoxicity, they reinforce the importance of in vitro toxicology in exposure assessments to determine the cytotoxicity of mixtures of contaminants, for better risk characterization and selection of appropriate risk management measures.ElsevierRCIPLViegas, CarlaTwarużek, MagdalenaDias, MartaAlmeida, BeatrizCarolino, ElisabeteSoszczyńska, EwelinaAłtyn, IwonaViegas, SusanaCaetano, Liliana Aranha2020-08-31T13:46:13Z2020-082020-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/12198engViegas C, Dias M, Almeida B, Carolino E, Viegas S, Caetano LA, et al. Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization? Environ Res. 2020;191:110134.10.1016/j.envres.2020.110134info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:04:39Zoai:repositorio.ipl.pt:10400.21/12198Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:20:20.105226Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?
title Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?
spellingShingle Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?
Viegas, Carla
Occupational health
Occupational exposure
Filtering respiratory protective devices
Waste sorting industry
In vitro models
Cytotoxic potential
Risk characterization
Risk management
Project nº 23222 (02/SAICT/2016)
Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”
Project IPL/2018/WasteFRPD_ESTeSL
title_short Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?
title_full Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?
title_fullStr Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?
title_full_unstemmed Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?
title_sort Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?
author Viegas, Carla
author_facet Viegas, Carla
Twarużek, Magdalena
Dias, Marta
Almeida, Beatriz
Carolino, Elisabete
Soszczyńska, Ewelina
Ałtyn, Iwona
Viegas, Susana
Caetano, Liliana Aranha
author_role author
author2 Twarużek, Magdalena
Dias, Marta
Almeida, Beatriz
Carolino, Elisabete
Soszczyńska, Ewelina
Ałtyn, Iwona
Viegas, Susana
Caetano, Liliana Aranha
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Viegas, Carla
Twarużek, Magdalena
Dias, Marta
Almeida, Beatriz
Carolino, Elisabete
Soszczyńska, Ewelina
Ałtyn, Iwona
Viegas, Susana
Caetano, Liliana Aranha
dc.subject.por.fl_str_mv Occupational health
Occupational exposure
Filtering respiratory protective devices
Waste sorting industry
In vitro models
Cytotoxic potential
Risk characterization
Risk management
Project nº 23222 (02/SAICT/2016)
Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”
Project IPL/2018/WasteFRPD_ESTeSL
topic Occupational health
Occupational exposure
Filtering respiratory protective devices
Waste sorting industry
In vitro models
Cytotoxic potential
Risk characterization
Risk management
Project nº 23222 (02/SAICT/2016)
Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”
Project IPL/2018/WasteFRPD_ESTeSL
description Project nº 23222 (02/SAICT/2016).
publishDate 2020
dc.date.none.fl_str_mv 2020-08-31T13:46:13Z
2020-08
2020-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/12198
url http://hdl.handle.net/10400.21/12198
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Viegas C, Dias M, Almeida B, Carolino E, Viegas S, Caetano LA, et al. Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization? Environ Res. 2020;191:110134.
10.1016/j.envres.2020.110134
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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