Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii

Detalhes bibliográficos
Autor(a) principal: Silva, Rui
Data de Publicação: 2015
Outros Autores: Aguiar, Tatiana Quinta, Domingues, Lucília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31736
Resumo: "Accepted 7 November 2014; Available online 15 November 2014"
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spelling Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypiiAshbya gossypiiPyrimidine pathwayRiboflavin productionUridine/uracil auxotrophyNutritional stressScience & Technology"Accepted 7 November 2014; Available online 15 November 2014"The Ashbya gossypii riboflavin biosynthetic pathway and its connection with the purine pathway have been well studied. However, the outcome of genetic alterations in the pyrimidine pathway on riboflavin production by A. gossypii had not yet been assessed. Here, we report that the blockage of the de novo pyrimidine biosynthetic pathway in the recently generated A. gossypii Agura3 uridine/uracil auxotrophic strain led to improved riboflavin production on standard agar-solidified complex medium. When extra uridine/uracil was supplied, the production of riboflavin by this auxotroph was repressed. High concentrations of uracil hampered this (and the parent) strain growth, whereas excess uridine favored the A. gossypii Agura3 growth. Considering that the riboflavin and the pyrimidine pathways share the same precursors and that riboflavin overproduction may be triggered by nutritional stress, we suggest that overproduction of riboflavin by the A. gossypii Agura3 may occur as an outcome of a nutritional stress response and/or of an increased availability in precursors for riboflavin biosynthesis, due to their reduced consumption by the pyrimidine pathway.This work was supported by Fundacao para a Ciencia e a Tecnologia, Portugal, through Project AshByofactory PTDC/EBB-EBI/101985/2008 - FCOMP-01-0124-FEDER-009701, Project Gly-coCBMs FCTPTDC/AGR-FOR/3090/2012 - FCOMP-01-0124-FEDER027948, Strategic Project PEst-OE/EQB/LA0023/2013, and Project BioInd NORTE-07-0124-FEDER-000028 co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER. The technical support provided by Claudia Dinis is also acknowledged.ElsevierUniversidade do MinhoSilva, RuiAguiar, Tatiana QuintaDomingues, Lucília20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31736eng0168-16560168-165610.1016/j.jbiotec.2014.11.00925444878info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:03Zoai:repositorium.sdum.uminho.pt:1822/31736Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:56:40.267179Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii
title Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii
spellingShingle Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii
Silva, Rui
Ashbya gossypii
Pyrimidine pathway
Riboflavin production
Uridine/uracil auxotrophy
Nutritional stress
Science & Technology
title_short Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii
title_full Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii
title_fullStr Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii
title_full_unstemmed Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii
title_sort Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii
author Silva, Rui
author_facet Silva, Rui
Aguiar, Tatiana Quinta
Domingues, Lucília
author_role author
author2 Aguiar, Tatiana Quinta
Domingues, Lucília
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Rui
Aguiar, Tatiana Quinta
Domingues, Lucília
dc.subject.por.fl_str_mv Ashbya gossypii
Pyrimidine pathway
Riboflavin production
Uridine/uracil auxotrophy
Nutritional stress
Science & Technology
topic Ashbya gossypii
Pyrimidine pathway
Riboflavin production
Uridine/uracil auxotrophy
Nutritional stress
Science & Technology
description "Accepted 7 November 2014; Available online 15 November 2014"
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31736
url http://hdl.handle.net/1822/31736
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0168-1656
0168-1656
10.1016/j.jbiotec.2014.11.009
25444878
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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