Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/6738 |
Resumo: | The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a β-galactosidase activity. |
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Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianusCre-loxP systemKluyveromyces marxianusGene disruptionβ-GalactosidaseGene targeting efficiencybeta-galactosidaseScience & TechnologyThe yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a β-galactosidase activity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - (CAPES), Brazil;Agência de Inovação - projecto UMINHO/POCI-Zimlac/BI/2/05Elsevier B.V.Universidade do MinhoRibeiro, OrquídeaGombert, Andreas KarolyTeixeira, J. A.Domingues, Lucília2007-082007-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/6738engRIBEIRO, Orquídea [et al.] - Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus. "Journal of Biotechnology" [Em linha]. 131:1 (Ago. 2007) 20-26. [Consult. 19 Jul. 2007. Disponível em WWW:<URL: http://www.sciencedirect.com/science/journal/01681656. ISSN 0168-1656.0168-165610.1016/j.jbiotec.2007.05.02717624462http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:08Zoai:repositorium.sdum.uminho.pt:1822/6738Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:41.649863Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus |
title |
Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus |
spellingShingle |
Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus Ribeiro, Orquídea Cre-loxP system Kluyveromyces marxianus Gene disruption β-Galactosidase Gene targeting efficiency beta-galactosidase Science & Technology |
title_short |
Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus |
title_full |
Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus |
title_fullStr |
Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus |
title_full_unstemmed |
Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus |
title_sort |
Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus |
author |
Ribeiro, Orquídea |
author_facet |
Ribeiro, Orquídea Gombert, Andreas Karoly Teixeira, J. A. Domingues, Lucília |
author_role |
author |
author2 |
Gombert, Andreas Karoly Teixeira, J. A. Domingues, Lucília |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ribeiro, Orquídea Gombert, Andreas Karoly Teixeira, J. A. Domingues, Lucília |
dc.subject.por.fl_str_mv |
Cre-loxP system Kluyveromyces marxianus Gene disruption β-Galactosidase Gene targeting efficiency beta-galactosidase Science & Technology |
topic |
Cre-loxP system Kluyveromyces marxianus Gene disruption β-Galactosidase Gene targeting efficiency beta-galactosidase Science & Technology |
description |
The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a β-galactosidase activity. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-08 2007-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/6738 |
url |
http://hdl.handle.net/1822/6738 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
RIBEIRO, Orquídea [et al.] - Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus. "Journal of Biotechnology" [Em linha]. 131:1 (Ago. 2007) 20-26. [Consult. 19 Jul. 2007. Disponível em WWW:<URL: http://www.sciencedirect.com/science/journal/01681656. ISSN 0168-1656. 0168-1656 10.1016/j.jbiotec.2007.05.027 17624462 http://www.sciencedirect.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132523572232192 |