Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/77692 |
Resumo: | Portable analytical systems are versatile tools for application in areas including biomedicine, biosecurity, food safety and environmental monitoring. This work contributes to the increasing demand for low-cost, environmentally friendly substrates for portable analytical systems by using natural Bombyx mori cocoons. Further, silk fibroin is also extracted from these cocoons and electrospun into oriented and randomly oriented fiber substrates. Oxygen plasma treatment is applied to improve their hydrophilicity. Fiber morphology, mechanical properties, porosity, thermal characteristics and surface contact angle are extensively characterized and the ability of the samples for passive capillary flows demonstrated. Plasma treated pressed cocoons show superhydrophilicity, capillary flow rates of 44.8 ± 3.75 mm.min-1, and high mechanical resistance with Young's modulus values up to 592.13 ± 19.83 MPa. The developed materials are used as substrates for the colorimetric quantification of three commonly scrutinized clinical analytes. Hydrophobic barriers are first wax-printed on all samples with a proper design and albumin assays are performed on all substrates. Further assays for uric acid and glucose quantification are successfully accomplished on the pressed cocoons after a simple in between washing step, with overall high coefficient of determination, proving the suitability of the developed materials as low-cost, sustainable and reusable microfluidic substrates. |
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Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systemsMicrofluidicPoint-of-carePortable analytical devicesSilkSustainabilityScience & TechnologyPortable analytical systems are versatile tools for application in areas including biomedicine, biosecurity, food safety and environmental monitoring. This work contributes to the increasing demand for low-cost, environmentally friendly substrates for portable analytical systems by using natural Bombyx mori cocoons. Further, silk fibroin is also extracted from these cocoons and electrospun into oriented and randomly oriented fiber substrates. Oxygen plasma treatment is applied to improve their hydrophilicity. Fiber morphology, mechanical properties, porosity, thermal characteristics and surface contact angle are extensively characterized and the ability of the samples for passive capillary flows demonstrated. Plasma treated pressed cocoons show superhydrophilicity, capillary flow rates of 44.8 ± 3.75 mm.min-1, and high mechanical resistance with Young's modulus values up to 592.13 ± 19.83 MPa. The developed materials are used as substrates for the colorimetric quantification of three commonly scrutinized clinical analytes. Hydrophobic barriers are first wax-printed on all samples with a proper design and albumin assays are performed on all substrates. Further assays for uric acid and glucose quantification are successfully accomplished on the pressed cocoons after a simple in between washing step, with overall high coefficient of determination, proving the suitability of the developed materials as low-cost, sustainable and reusable microfluidic substrates.This work was supported by the Portuguese Foundation for Science and Technology (FCT) under strategic funding UID/FIS/04650/2020, UIDB/04436/2020, UIDP/04436/2020 and project PTDC/EMD-EMD/28159/2017 (POCI-01–0145-FEDER-028159). The authors also thank FCT for financial support under grants SFRH/BD/140698/2018 (R.B. P.), 2020.09218.BD (A.S.M.), 2020.04163.CEECIND (C.R.) and 2020.02304.CEECIND (V.F.C.). Finally, the authors acknowledge fund ing by Spanish State Research Agency (AEI) and the European Regional Development Fund (ERFD) through the project PID2019–106099RB C43/AEI/10.13039/501100011033 and from the Basque Government Industry Departments under the ELKARTEK program. Finally, the au thors also thank Dr. J. Borges and Prof. F. Vaz for experimental support.info:eu-repo/semantics/publishedVersionElsevier BVUniversidade do MinhoBrito-Pereira, RicardoMacedo, André S.Ribeiro, Clarisse Marta OliveiraCardoso, Vanessa FernandesLanceros-Méndez, S.2022-082022-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/77692engBrito-Pereira, Ricardo; Macedo, André S.; Ribeiro, Clarisse; Cardoso, Vanessa F.; Lanceros-Méndez, Senentxu, Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems. Applied Materials Today, 28(101507), 20222352-940710.1016/j.apmt.2022.101507101507https://www.sciencedirect.com/science/article/pii/S2352940722001421info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:15Zoai:repositorium.sdum.uminho.pt:1822/77692Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:37:00.775729Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems |
title |
Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems |
spellingShingle |
Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems Brito-Pereira, Ricardo Microfluidic Point-of-care Portable analytical devices Silk Sustainability Science & Technology |
title_short |
Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems |
title_full |
Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems |
title_fullStr |
Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems |
title_full_unstemmed |
Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems |
title_sort |
Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems |
author |
Brito-Pereira, Ricardo |
author_facet |
Brito-Pereira, Ricardo Macedo, André S. Ribeiro, Clarisse Marta Oliveira Cardoso, Vanessa Fernandes Lanceros-Méndez, S. |
author_role |
author |
author2 |
Macedo, André S. Ribeiro, Clarisse Marta Oliveira Cardoso, Vanessa Fernandes Lanceros-Méndez, S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Brito-Pereira, Ricardo Macedo, André S. Ribeiro, Clarisse Marta Oliveira Cardoso, Vanessa Fernandes Lanceros-Méndez, S. |
dc.subject.por.fl_str_mv |
Microfluidic Point-of-care Portable analytical devices Silk Sustainability Science & Technology |
topic |
Microfluidic Point-of-care Portable analytical devices Silk Sustainability Science & Technology |
description |
Portable analytical systems are versatile tools for application in areas including biomedicine, biosecurity, food safety and environmental monitoring. This work contributes to the increasing demand for low-cost, environmentally friendly substrates for portable analytical systems by using natural Bombyx mori cocoons. Further, silk fibroin is also extracted from these cocoons and electrospun into oriented and randomly oriented fiber substrates. Oxygen plasma treatment is applied to improve their hydrophilicity. Fiber morphology, mechanical properties, porosity, thermal characteristics and surface contact angle are extensively characterized and the ability of the samples for passive capillary flows demonstrated. Plasma treated pressed cocoons show superhydrophilicity, capillary flow rates of 44.8 ± 3.75 mm.min-1, and high mechanical resistance with Young's modulus values up to 592.13 ± 19.83 MPa. The developed materials are used as substrates for the colorimetric quantification of three commonly scrutinized clinical analytes. Hydrophobic barriers are first wax-printed on all samples with a proper design and albumin assays are performed on all substrates. Further assays for uric acid and glucose quantification are successfully accomplished on the pressed cocoons after a simple in between washing step, with overall high coefficient of determination, proving the suitability of the developed materials as low-cost, sustainable and reusable microfluidic substrates. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08 2022-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/77692 |
url |
https://hdl.handle.net/1822/77692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brito-Pereira, Ricardo; Macedo, André S.; Ribeiro, Clarisse; Cardoso, Vanessa F.; Lanceros-Méndez, Senentxu, Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems. Applied Materials Today, 28(101507), 2022 2352-9407 10.1016/j.apmt.2022.101507 101507 https://www.sciencedirect.com/science/article/pii/S2352940722001421 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier BV |
publisher.none.fl_str_mv |
Elsevier BV |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132902374506496 |