Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems

Detalhes bibliográficos
Autor(a) principal: Brito-Pereira, Ricardo
Data de Publicação: 2022
Outros Autores: Macedo, André S., Ribeiro, Clarisse Marta Oliveira, Cardoso, Vanessa Fernandes, Lanceros-Méndez, S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/77692
Resumo: Portable analytical systems are versatile tools for application in areas including biomedicine, biosecurity, food safety and environmental monitoring. This work contributes to the increasing demand for low-cost, environmentally friendly substrates for portable analytical systems by using natural Bombyx mori cocoons. Further, silk fibroin is also extracted from these cocoons and electrospun into oriented and randomly oriented fiber substrates. Oxygen plasma treatment is applied to improve their hydrophilicity. Fiber morphology, mechanical properties, porosity, thermal characteristics and surface contact angle are extensively characterized and the ability of the samples for passive capillary flows demonstrated. Plasma treated pressed cocoons show superhydrophilicity, capillary flow rates of 44.8 ± 3.75 mm.min-1, and high mechanical resistance with Young's modulus values up to 592.13 ± 19.83 MPa. The developed materials are used as substrates for the colorimetric quantification of three commonly scrutinized clinical analytes. Hydrophobic barriers are first wax-printed on all samples with a proper design and albumin assays are performed on all substrates. Further assays for uric acid and glucose quantification are successfully accomplished on the pressed cocoons after a simple in between washing step, with overall high coefficient of determination, proving the suitability of the developed materials as low-cost, sustainable and reusable microfluidic substrates.
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spelling Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systemsMicrofluidicPoint-of-carePortable analytical devicesSilkSustainabilityScience & TechnologyPortable analytical systems are versatile tools for application in areas including biomedicine, biosecurity, food safety and environmental monitoring. This work contributes to the increasing demand for low-cost, environmentally friendly substrates for portable analytical systems by using natural Bombyx mori cocoons. Further, silk fibroin is also extracted from these cocoons and electrospun into oriented and randomly oriented fiber substrates. Oxygen plasma treatment is applied to improve their hydrophilicity. Fiber morphology, mechanical properties, porosity, thermal characteristics and surface contact angle are extensively characterized and the ability of the samples for passive capillary flows demonstrated. Plasma treated pressed cocoons show superhydrophilicity, capillary flow rates of 44.8 ± 3.75 mm.min-1, and high mechanical resistance with Young's modulus values up to 592.13 ± 19.83 MPa. The developed materials are used as substrates for the colorimetric quantification of three commonly scrutinized clinical analytes. Hydrophobic barriers are first wax-printed on all samples with a proper design and albumin assays are performed on all substrates. Further assays for uric acid and glucose quantification are successfully accomplished on the pressed cocoons after a simple in between washing step, with overall high coefficient of determination, proving the suitability of the developed materials as low-cost, sustainable and reusable microfluidic substrates.This work was supported by the Portuguese Foundation for Science and Technology (FCT) under strategic funding UID/FIS/04650/2020, UIDB/04436/2020, UIDP/04436/2020 and project PTDC/EMD-EMD/28159/2017 (POCI-01–0145-FEDER-028159). The authors also thank FCT for financial support under grants SFRH/BD/140698/2018 (R.B. P.), 2020.09218.BD (A.S.M.), 2020.04163.CEECIND (C.R.) and 2020.02304.CEECIND (V.F.C.). Finally, the authors acknowledge fund ing by Spanish State Research Agency (AEI) and the European Regional Development Fund (ERFD) through the project PID2019–106099RB C43/AEI/10.13039/501100011033 and from the Basque Government Industry Departments under the ELKARTEK program. Finally, the au thors also thank Dr. J. Borges and Prof. F. Vaz for experimental support.info:eu-repo/semantics/publishedVersionElsevier BVUniversidade do MinhoBrito-Pereira, RicardoMacedo, André S.Ribeiro, Clarisse Marta OliveiraCardoso, Vanessa FernandesLanceros-Méndez, S.2022-082022-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/77692engBrito-Pereira, Ricardo; Macedo, André S.; Ribeiro, Clarisse; Cardoso, Vanessa F.; Lanceros-Méndez, Senentxu, Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems. Applied Materials Today, 28(101507), 20222352-940710.1016/j.apmt.2022.101507101507https://www.sciencedirect.com/science/article/pii/S2352940722001421info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:15Zoai:repositorium.sdum.uminho.pt:1822/77692Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:37:00.775729Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems
title Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems
spellingShingle Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems
Brito-Pereira, Ricardo
Microfluidic
Point-of-care
Portable analytical devices
Silk
Sustainability
Science & Technology
title_short Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems
title_full Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems
title_fullStr Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems
title_full_unstemmed Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems
title_sort Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems
author Brito-Pereira, Ricardo
author_facet Brito-Pereira, Ricardo
Macedo, André S.
Ribeiro, Clarisse Marta Oliveira
Cardoso, Vanessa Fernandes
Lanceros-Méndez, S.
author_role author
author2 Macedo, André S.
Ribeiro, Clarisse Marta Oliveira
Cardoso, Vanessa Fernandes
Lanceros-Méndez, S.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Brito-Pereira, Ricardo
Macedo, André S.
Ribeiro, Clarisse Marta Oliveira
Cardoso, Vanessa Fernandes
Lanceros-Méndez, S.
dc.subject.por.fl_str_mv Microfluidic
Point-of-care
Portable analytical devices
Silk
Sustainability
Science & Technology
topic Microfluidic
Point-of-care
Portable analytical devices
Silk
Sustainability
Science & Technology
description Portable analytical systems are versatile tools for application in areas including biomedicine, biosecurity, food safety and environmental monitoring. This work contributes to the increasing demand for low-cost, environmentally friendly substrates for portable analytical systems by using natural Bombyx mori cocoons. Further, silk fibroin is also extracted from these cocoons and electrospun into oriented and randomly oriented fiber substrates. Oxygen plasma treatment is applied to improve their hydrophilicity. Fiber morphology, mechanical properties, porosity, thermal characteristics and surface contact angle are extensively characterized and the ability of the samples for passive capillary flows demonstrated. Plasma treated pressed cocoons show superhydrophilicity, capillary flow rates of 44.8 ± 3.75 mm.min-1, and high mechanical resistance with Young's modulus values up to 592.13 ± 19.83 MPa. The developed materials are used as substrates for the colorimetric quantification of three commonly scrutinized clinical analytes. Hydrophobic barriers are first wax-printed on all samples with a proper design and albumin assays are performed on all substrates. Further assays for uric acid and glucose quantification are successfully accomplished on the pressed cocoons after a simple in between washing step, with overall high coefficient of determination, proving the suitability of the developed materials as low-cost, sustainable and reusable microfluidic substrates.
publishDate 2022
dc.date.none.fl_str_mv 2022-08
2022-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/77692
url https://hdl.handle.net/1822/77692
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brito-Pereira, Ricardo; Macedo, André S.; Ribeiro, Clarisse; Cardoso, Vanessa F.; Lanceros-Méndez, Senentxu, Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems. Applied Materials Today, 28(101507), 2022
2352-9407
10.1016/j.apmt.2022.101507
101507
https://www.sciencedirect.com/science/article/pii/S2352940722001421
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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