Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests

Detalhes bibliográficos
Autor(a) principal: Moreno, Yorleydy Ruiz
Data de Publicação: 2019
Outros Autores: Donato, Silvia Tavares, Nogueira, Fátima, Silva, Marcelo Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116623
Resumo: Early diagnosis of malaria reduces disease, prevents deaths, and contributes to decreased malaria transmission. The use of specific and sensitive antigens in the execution of serological diagnostics may have an impact on the transmission of the disease. However, many individuals cannot be easily diagnosed by serological tests due to low levels of antibodies in the serum. Using two different Enzyme-Linked Immunosorbent Assay (ELISA) tests (a commercial and an in-house ELISA), a total of 365 serum samples from individuals with a clinical history of malaria were analyzed. From the serum samples analyzed, 192 (53%) samples from the commercial ELISA and 219 (60%) samples from the in-house ELISA presented positive serological reactivity to malaria. The concordance of the samples tested (n = 365) between both ELISAs was of 67% (n = 242), and with the negative control was 100% (n = 17). We demonstrated that the in-house ELISA showed high antigenic reactivity to Plasmodium falciparum antigens when compared with the commercial ELISA. The degree of concordance of both ELISAs suggested the possibility of existence of other P. falciparum antigens present in the crude extract of P. falciparum that are important in the serological response during malaria infection.
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spelling Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA testsELISAMalariaPlasmodium falciparumSerological diagnosisSerological markersClinical BiochemistryImmunologyInfectious DiseasesSDG 3 - Good Health and Well-beingEarly diagnosis of malaria reduces disease, prevents deaths, and contributes to decreased malaria transmission. The use of specific and sensitive antigens in the execution of serological diagnostics may have an impact on the transmission of the disease. However, many individuals cannot be easily diagnosed by serological tests due to low levels of antibodies in the serum. Using two different Enzyme-Linked Immunosorbent Assay (ELISA) tests (a commercial and an in-house ELISA), a total of 365 serum samples from individuals with a clinical history of malaria were analyzed. From the serum samples analyzed, 192 (53%) samples from the commercial ELISA and 219 (60%) samples from the in-house ELISA presented positive serological reactivity to malaria. The concordance of the samples tested (n = 365) between both ELISAs was of 67% (n = 242), and with the negative control was 100% (n = 17). We demonstrated that the in-house ELISA showed high antigenic reactivity to Plasmodium falciparum antigens when compared with the commercial ELISA. The degree of concordance of both ELISAs suggested the possibility of existence of other P. falciparum antigens present in the crude extract of P. falciparum that are important in the serological response during malaria infection.Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNMoreno, Yorleydy RuizDonato, Silvia TavaresNogueira, FátimaSilva, Marcelo Sousa2021-05-01T22:52:45Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/116623eng2075-4418PURE: 26649719https://doi.org/10.3390/diagnostics9040168info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:17Zoai:run.unl.pt:10362/116623Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:10.559663Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests
title Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests
spellingShingle Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests
Moreno, Yorleydy Ruiz
ELISA
Malaria
Plasmodium falciparum
Serological diagnosis
Serological markers
Clinical Biochemistry
Immunology
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests
title_full Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests
title_fullStr Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests
title_full_unstemmed Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests
title_sort Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests
author Moreno, Yorleydy Ruiz
author_facet Moreno, Yorleydy Ruiz
Donato, Silvia Tavares
Nogueira, Fátima
Silva, Marcelo Sousa
author_role author
author2 Donato, Silvia Tavares
Nogueira, Fátima
Silva, Marcelo Sousa
author2_role author
author
author
dc.contributor.none.fl_str_mv Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Moreno, Yorleydy Ruiz
Donato, Silvia Tavares
Nogueira, Fátima
Silva, Marcelo Sousa
dc.subject.por.fl_str_mv ELISA
Malaria
Plasmodium falciparum
Serological diagnosis
Serological markers
Clinical Biochemistry
Immunology
Infectious Diseases
SDG 3 - Good Health and Well-being
topic ELISA
Malaria
Plasmodium falciparum
Serological diagnosis
Serological markers
Clinical Biochemistry
Immunology
Infectious Diseases
SDG 3 - Good Health and Well-being
description Early diagnosis of malaria reduces disease, prevents deaths, and contributes to decreased malaria transmission. The use of specific and sensitive antigens in the execution of serological diagnostics may have an impact on the transmission of the disease. However, many individuals cannot be easily diagnosed by serological tests due to low levels of antibodies in the serum. Using two different Enzyme-Linked Immunosorbent Assay (ELISA) tests (a commercial and an in-house ELISA), a total of 365 serum samples from individuals with a clinical history of malaria were analyzed. From the serum samples analyzed, 192 (53%) samples from the commercial ELISA and 219 (60%) samples from the in-house ELISA presented positive serological reactivity to malaria. The concordance of the samples tested (n = 365) between both ELISAs was of 67% (n = 242), and with the negative control was 100% (n = 17). We demonstrated that the in-house ELISA showed high antigenic reactivity to Plasmodium falciparum antigens when compared with the commercial ELISA. The degree of concordance of both ELISAs suggested the possibility of existence of other P. falciparum antigens present in the crude extract of P. falciparum that are important in the serological response during malaria infection.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
2019-12-01T00:00:00Z
2021-05-01T22:52:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116623
url http://hdl.handle.net/10362/116623
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2075-4418
PURE: 26649719
https://doi.org/10.3390/diagnostics9040168
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eu_rights_str_mv openAccess
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