Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/116623 |
Resumo: | Early diagnosis of malaria reduces disease, prevents deaths, and contributes to decreased malaria transmission. The use of specific and sensitive antigens in the execution of serological diagnostics may have an impact on the transmission of the disease. However, many individuals cannot be easily diagnosed by serological tests due to low levels of antibodies in the serum. Using two different Enzyme-Linked Immunosorbent Assay (ELISA) tests (a commercial and an in-house ELISA), a total of 365 serum samples from individuals with a clinical history of malaria were analyzed. From the serum samples analyzed, 192 (53%) samples from the commercial ELISA and 219 (60%) samples from the in-house ELISA presented positive serological reactivity to malaria. The concordance of the samples tested (n = 365) between both ELISAs was of 67% (n = 242), and with the negative control was 100% (n = 17). We demonstrated that the in-house ELISA showed high antigenic reactivity to Plasmodium falciparum antigens when compared with the commercial ELISA. The degree of concordance of both ELISAs suggested the possibility of existence of other P. falciparum antigens present in the crude extract of P. falciparum that are important in the serological response during malaria infection. |
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Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA testsELISAMalariaPlasmodium falciparumSerological diagnosisSerological markersClinical BiochemistryImmunologyInfectious DiseasesSDG 3 - Good Health and Well-beingEarly diagnosis of malaria reduces disease, prevents deaths, and contributes to decreased malaria transmission. The use of specific and sensitive antigens in the execution of serological diagnostics may have an impact on the transmission of the disease. However, many individuals cannot be easily diagnosed by serological tests due to low levels of antibodies in the serum. Using two different Enzyme-Linked Immunosorbent Assay (ELISA) tests (a commercial and an in-house ELISA), a total of 365 serum samples from individuals with a clinical history of malaria were analyzed. From the serum samples analyzed, 192 (53%) samples from the commercial ELISA and 219 (60%) samples from the in-house ELISA presented positive serological reactivity to malaria. The concordance of the samples tested (n = 365) between both ELISAs was of 67% (n = 242), and with the negative control was 100% (n = 17). We demonstrated that the in-house ELISA showed high antigenic reactivity to Plasmodium falciparum antigens when compared with the commercial ELISA. The degree of concordance of both ELISAs suggested the possibility of existence of other P. falciparum antigens present in the crude extract of P. falciparum that are important in the serological response during malaria infection.Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNMoreno, Yorleydy RuizDonato, Silvia TavaresNogueira, FátimaSilva, Marcelo Sousa2021-05-01T22:52:45Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/116623eng2075-4418PURE: 26649719https://doi.org/10.3390/diagnostics9040168info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:17Zoai:run.unl.pt:10362/116623Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:10.559663Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests |
title |
Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests |
spellingShingle |
Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests Moreno, Yorleydy Ruiz ELISA Malaria Plasmodium falciparum Serological diagnosis Serological markers Clinical Biochemistry Immunology Infectious Diseases SDG 3 - Good Health and Well-being |
title_short |
Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests |
title_full |
Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests |
title_fullStr |
Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests |
title_full_unstemmed |
Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests |
title_sort |
Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests |
author |
Moreno, Yorleydy Ruiz |
author_facet |
Moreno, Yorleydy Ruiz Donato, Silvia Tavares Nogueira, Fátima Silva, Marcelo Sousa |
author_role |
author |
author2 |
Donato, Silvia Tavares Nogueira, Fátima Silva, Marcelo Sousa |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Global Health and Tropical Medicine (GHTM) Instituto de Higiene e Medicina Tropical (IHMT) RUN |
dc.contributor.author.fl_str_mv |
Moreno, Yorleydy Ruiz Donato, Silvia Tavares Nogueira, Fátima Silva, Marcelo Sousa |
dc.subject.por.fl_str_mv |
ELISA Malaria Plasmodium falciparum Serological diagnosis Serological markers Clinical Biochemistry Immunology Infectious Diseases SDG 3 - Good Health and Well-being |
topic |
ELISA Malaria Plasmodium falciparum Serological diagnosis Serological markers Clinical Biochemistry Immunology Infectious Diseases SDG 3 - Good Health and Well-being |
description |
Early diagnosis of malaria reduces disease, prevents deaths, and contributes to decreased malaria transmission. The use of specific and sensitive antigens in the execution of serological diagnostics may have an impact on the transmission of the disease. However, many individuals cannot be easily diagnosed by serological tests due to low levels of antibodies in the serum. Using two different Enzyme-Linked Immunosorbent Assay (ELISA) tests (a commercial and an in-house ELISA), a total of 365 serum samples from individuals with a clinical history of malaria were analyzed. From the serum samples analyzed, 192 (53%) samples from the commercial ELISA and 219 (60%) samples from the in-house ELISA presented positive serological reactivity to malaria. The concordance of the samples tested (n = 365) between both ELISAs was of 67% (n = 242), and with the negative control was 100% (n = 17). We demonstrated that the in-house ELISA showed high antigenic reactivity to Plasmodium falciparum antigens when compared with the commercial ELISA. The degree of concordance of both ELISAs suggested the possibility of existence of other P. falciparum antigens present in the crude extract of P. falciparum that are important in the serological response during malaria infection. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 2019-12-01T00:00:00Z 2021-05-01T22:52:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/116623 |
url |
http://hdl.handle.net/10362/116623 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2075-4418 PURE: 26649719 https://doi.org/10.3390/diagnostics9040168 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799138041915244544 |