Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/15359 |
Resumo: | BACKGROUND Morus nigra (mulberry) fruit has been reported as a source of bioactive compounds, although information about their leaves is very limited. Usually, they are considered wastes and have been traditionally used only for tea preparation. The main objective of this work was to explore the valorization of mulberry leaves by polyphenols microwave‐assisted extraction (MAE) and characterization of their antioxidant activity and phenolic composition. A 23 factorial design combined with response surface methodology were applied to characterize the effect of main microwave parameters on total phenolic content (TPC). RESULTS The optimized MAE conditions were 20 mL of ethanol:water (1:1; v/v), 120 °C, 28 min, 0.414 g and medium stirring speed. Under these conditions, TPC was 19.7 ± 2.0 mg gallic acid equivalents (GAE) g‐1 dry plant (DP), and antioxidant activity was 15.3 ± 1.0 mg ascorbic acid (AA) g‐1 DP (ferric reduction activity power –FRAP– assay), 18.6 ± 1.3 mg Trolox equivalents (TE) g‐1 DP (2,2‐diphenyl‐1‐picrylhydrazyl –DPPH– assay) and 186 ± 15 mg TE g‐1 DP (oxygen radical absorbance capacity –ORAC– assay). When compared with ultrasound extraction and Pharmacopeia reference method, MAE was more efficient, representing a valuable technology. Of the 13 compounds identified by HPLC, the most abundant were rutin, chlorogenic, β‐resorcylic and caffeic acids. CONCLUSION These results indicate that mulberry leaves are of potential interest for agro‐food industries as a new source of natural antioxidants. |
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Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic compositionMorus nigra leavesMicrowave-assisted extractionResponse surface methodologyAntioxidant activityPhenolic compoundsBACKGROUND Morus nigra (mulberry) fruit has been reported as a source of bioactive compounds, although information about their leaves is very limited. Usually, they are considered wastes and have been traditionally used only for tea preparation. The main objective of this work was to explore the valorization of mulberry leaves by polyphenols microwave‐assisted extraction (MAE) and characterization of their antioxidant activity and phenolic composition. A 23 factorial design combined with response surface methodology were applied to characterize the effect of main microwave parameters on total phenolic content (TPC). RESULTS The optimized MAE conditions were 20 mL of ethanol:water (1:1; v/v), 120 °C, 28 min, 0.414 g and medium stirring speed. Under these conditions, TPC was 19.7 ± 2.0 mg gallic acid equivalents (GAE) g‐1 dry plant (DP), and antioxidant activity was 15.3 ± 1.0 mg ascorbic acid (AA) g‐1 DP (ferric reduction activity power –FRAP– assay), 18.6 ± 1.3 mg Trolox equivalents (TE) g‐1 DP (2,2‐diphenyl‐1‐picrylhydrazyl –DPPH– assay) and 186 ± 15 mg TE g‐1 DP (oxygen radical absorbance capacity –ORAC– assay). When compared with ultrasound extraction and Pharmacopeia reference method, MAE was more efficient, representing a valuable technology. Of the 13 compounds identified by HPLC, the most abundant were rutin, chlorogenic, β‐resorcylic and caffeic acids. CONCLUSION These results indicate that mulberry leaves are of potential interest for agro‐food industries as a new source of natural antioxidants.This investigation is a part of the postdoctoral research by Marija Radojkovic, and she is grateful for financial support by ´the Serbian Ministry of Education, Science and Technological Development (Project No. TR 31013); the project 6818 - Transnational Cooperation Agreement between Portugal (FCT) and Serbia (MSTD) is also acknowledged. Authors are also grateful for financial support from project NORTE-01-0145-FEDER-000011 –311 Qualidade e Segurança Alimentar – uma abordagem (nano) tecnológica. The financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 through the project UID/ QUI/50006/2013 - POCI/01/0145/FERDER/007265, is also acknowledged. M. F. Barroso (SFRH/BPD/78845/2011) and Manuela M. Moreira (SFRH/BPD/97049/2013) are grateful to Fundação para a Ciência e Tecnologia for their postdoctoral fellowships.Society of Chemical IndustryRepositório Científico do Instituto Politécnico do PortoRadojković, MarijaMoreira, Manuela M.Soares, CristinaBarroso, M. FátimaCvetanović, AleksandraŠvarc-Gajić, JaroslavaMorais, SimoneDelerue-Matos, Cristina2020-01-23T15:23:25Z2018-022018-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/15359eng1097-466010.1002/jctb.5541info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:57:43Zoai:recipp.ipp.pt:10400.22/15359Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:34:14.065527Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition |
title |
Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition |
spellingShingle |
Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition Radojković, Marija Morus nigra leaves Microwave-assisted extraction Response surface methodology Antioxidant activity Phenolic compounds |
title_short |
Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition |
title_full |
Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition |
title_fullStr |
Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition |
title_full_unstemmed |
Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition |
title_sort |
Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition |
author |
Radojković, Marija |
author_facet |
Radojković, Marija Moreira, Manuela M. Soares, Cristina Barroso, M. Fátima Cvetanović, Aleksandra Švarc-Gajić, Jaroslava Morais, Simone Delerue-Matos, Cristina |
author_role |
author |
author2 |
Moreira, Manuela M. Soares, Cristina Barroso, M. Fátima Cvetanović, Aleksandra Švarc-Gajić, Jaroslava Morais, Simone Delerue-Matos, Cristina |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Radojković, Marija Moreira, Manuela M. Soares, Cristina Barroso, M. Fátima Cvetanović, Aleksandra Švarc-Gajić, Jaroslava Morais, Simone Delerue-Matos, Cristina |
dc.subject.por.fl_str_mv |
Morus nigra leaves Microwave-assisted extraction Response surface methodology Antioxidant activity Phenolic compounds |
topic |
Morus nigra leaves Microwave-assisted extraction Response surface methodology Antioxidant activity Phenolic compounds |
description |
BACKGROUND Morus nigra (mulberry) fruit has been reported as a source of bioactive compounds, although information about their leaves is very limited. Usually, they are considered wastes and have been traditionally used only for tea preparation. The main objective of this work was to explore the valorization of mulberry leaves by polyphenols microwave‐assisted extraction (MAE) and characterization of their antioxidant activity and phenolic composition. A 23 factorial design combined with response surface methodology were applied to characterize the effect of main microwave parameters on total phenolic content (TPC). RESULTS The optimized MAE conditions were 20 mL of ethanol:water (1:1; v/v), 120 °C, 28 min, 0.414 g and medium stirring speed. Under these conditions, TPC was 19.7 ± 2.0 mg gallic acid equivalents (GAE) g‐1 dry plant (DP), and antioxidant activity was 15.3 ± 1.0 mg ascorbic acid (AA) g‐1 DP (ferric reduction activity power –FRAP– assay), 18.6 ± 1.3 mg Trolox equivalents (TE) g‐1 DP (2,2‐diphenyl‐1‐picrylhydrazyl –DPPH– assay) and 186 ± 15 mg TE g‐1 DP (oxygen radical absorbance capacity –ORAC– assay). When compared with ultrasound extraction and Pharmacopeia reference method, MAE was more efficient, representing a valuable technology. Of the 13 compounds identified by HPLC, the most abundant were rutin, chlorogenic, β‐resorcylic and caffeic acids. CONCLUSION These results indicate that mulberry leaves are of potential interest for agro‐food industries as a new source of natural antioxidants. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02 2018-02-01T00:00:00Z 2020-01-23T15:23:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/15359 |
url |
http://hdl.handle.net/10400.22/15359 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1097-4660 10.1002/jctb.5541 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Society of Chemical Industry |
publisher.none.fl_str_mv |
Society of Chemical Industry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1817552656930963456 |