Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development

Detalhes bibliográficos
Autor(a) principal: Oliveira, Andreia C.
Data de Publicação: 2023
Outros Autores: Homem, Catarina C.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158603
Resumo: Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme ( H2020-ERC-2017-STG-GA 759853 -StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute ( HHMI-208581/Z/17/Z -Metabolic Reg SC fate); EMBO Installation grant ( H2020-EMBO-3311/2017/G2017 ); by Fundação para a Ciência e Tecnologia ( PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health — UIDB/04462/2020 and UIDP/04462/2020 , and by the Associated Laboratory LS4FUTURE ( LA/P/0087/2020 ), two programs financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência , Tecnologia e Ensino Superior . The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170 . Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute (HHMI-208581/Z/17/Z-Metabolic Reg SC fate); EMBO Installation grant (H2020-EMBO-3311/2017/G2017); by Fundação para a Ciência e Tecnologia (PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), two programs financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior. The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170. Publisher Copyright: © 2023 The Authors
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spelling Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism developmentBrainDrosophilaEcdysoneNeuroblastProliferationMolecular BiologyDevelopmental BiologyCell BiologyFunding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme ( H2020-ERC-2017-STG-GA 759853 -StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute ( HHMI-208581/Z/17/Z -Metabolic Reg SC fate); EMBO Installation grant ( H2020-EMBO-3311/2017/G2017 ); by Fundação para a Ciência e Tecnologia ( PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health — UIDB/04462/2020 and UIDP/04462/2020 , and by the Associated Laboratory LS4FUTURE ( LA/P/0087/2020 ), two programs financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência , Tecnologia e Ensino Superior . The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170 . Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute (HHMI-208581/Z/17/Z-Metabolic Reg SC fate); EMBO Installation grant (H2020-EMBO-3311/2017/G2017); by Fundação para a Ciência e Tecnologia (PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), two programs financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior. The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170. Publisher Copyright: © 2023 The AuthorsGrowth regulation must be robust to ensure correct final size, but also adaptative to adjust to less favorable environmental conditions. Developmental coordination between whole-organism and the brain is particularly important, as the brain is a critical organ with little adaptability. Brain growth mainly depends on neural stem cell (NSC) proliferation to generate differentiated neural cells, it is however unclear how organism developmental progression is coordinated with NSCs. Here we demonstrate that the steroid hormone ecdysone plays a multi-step, stage specific role in regulating Drosophila NSCs, the neuroblasts. We used animals that are unable to synthesize ecdysone, to show that the developmental milestone called “critical weight peak”, the peak that informs the body has reached minimum viable weight to survive metamorphosis, acts a checkpoint necessary to set neuroblast cell cycle pace during larval neurogenesis. The peaks of ecdysone that occur post-critical weight are no longer required to maintain neuroblast division rate. We additionally show that in a second stage, at the onset of pupariation, ecdysone is instead required to trigger neuroblast's proliferation exit and consequently the end of neurogenesis. We demonstrate that, without this signal from ecdysone, neuroblasts lose their ability to exit proliferation. Interestingly, although these neuroblasts proliferate for a longer period, the number of differentiated neurons is smaller compared to wild-type brains, suggesting a role for ecdysone in neuron maintenance. Our study provides insights into how neural stem cells coordinate their division rate with the pace of body growth, identifying a novel coordination mechanism between animal development and NSC proliferation.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)iNOVA4Health - pólo NMSRUNOliveira, Andreia C.Homem, Catarina C.F.2023-10-02T22:18:49Z2023-112023-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/158603eng0012-1606PURE: 72963015https://doi.org/10.1016/j.ydbio.2023.08.001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:08Zoai:run.unl.pt:10362/158603Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:13.725390Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development
title Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development
spellingShingle Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development
Oliveira, Andreia C.
Brain
Drosophila
Ecdysone
Neuroblast
Proliferation
Molecular Biology
Developmental Biology
Cell Biology
title_short Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development
title_full Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development
title_fullStr Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development
title_full_unstemmed Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development
title_sort Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development
author Oliveira, Andreia C.
author_facet Oliveira, Andreia C.
Homem, Catarina C.F.
author_role author
author2 Homem, Catarina C.F.
author2_role author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
iNOVA4Health - pólo NMS
RUN
dc.contributor.author.fl_str_mv Oliveira, Andreia C.
Homem, Catarina C.F.
dc.subject.por.fl_str_mv Brain
Drosophila
Ecdysone
Neuroblast
Proliferation
Molecular Biology
Developmental Biology
Cell Biology
topic Brain
Drosophila
Ecdysone
Neuroblast
Proliferation
Molecular Biology
Developmental Biology
Cell Biology
description Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme ( H2020-ERC-2017-STG-GA 759853 -StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute ( HHMI-208581/Z/17/Z -Metabolic Reg SC fate); EMBO Installation grant ( H2020-EMBO-3311/2017/G2017 ); by Fundação para a Ciência e Tecnologia ( PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health — UIDB/04462/2020 and UIDP/04462/2020 , and by the Associated Laboratory LS4FUTURE ( LA/P/0087/2020 ), two programs financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência , Tecnologia e Ensino Superior . The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170 . Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute (HHMI-208581/Z/17/Z-Metabolic Reg SC fate); EMBO Installation grant (H2020-EMBO-3311/2017/G2017); by Fundação para a Ciência e Tecnologia (PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), two programs financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior. The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170. Publisher Copyright: © 2023 The Authors
publishDate 2023
dc.date.none.fl_str_mv 2023-10-02T22:18:49Z
2023-11
2023-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158603
url http://hdl.handle.net/10362/158603
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0012-1606
PURE: 72963015
https://doi.org/10.1016/j.ydbio.2023.08.001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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