Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/158603 |
Resumo: | Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme ( H2020-ERC-2017-STG-GA 759853 -StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute ( HHMI-208581/Z/17/Z -Metabolic Reg SC fate); EMBO Installation grant ( H2020-EMBO-3311/2017/G2017 ); by Fundação para a Ciência e Tecnologia ( PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health — UIDB/04462/2020 and UIDP/04462/2020 , and by the Associated Laboratory LS4FUTURE ( LA/P/0087/2020 ), two programs financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência , Tecnologia e Ensino Superior . The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170 . Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute (HHMI-208581/Z/17/Z-Metabolic Reg SC fate); EMBO Installation grant (H2020-EMBO-3311/2017/G2017); by Fundação para a Ciência e Tecnologia (PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), two programs financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior. The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170. Publisher Copyright: © 2023 The Authors |
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Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism developmentBrainDrosophilaEcdysoneNeuroblastProliferationMolecular BiologyDevelopmental BiologyCell BiologyFunding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme ( H2020-ERC-2017-STG-GA 759853 -StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute ( HHMI-208581/Z/17/Z -Metabolic Reg SC fate); EMBO Installation grant ( H2020-EMBO-3311/2017/G2017 ); by Fundação para a Ciência e Tecnologia ( PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health — UIDB/04462/2020 and UIDP/04462/2020 , and by the Associated Laboratory LS4FUTURE ( LA/P/0087/2020 ), two programs financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência , Tecnologia e Ensino Superior . The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170 . Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute (HHMI-208581/Z/17/Z-Metabolic Reg SC fate); EMBO Installation grant (H2020-EMBO-3311/2017/G2017); by Fundação para a Ciência e Tecnologia (PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), two programs financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior. The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170. Publisher Copyright: © 2023 The AuthorsGrowth regulation must be robust to ensure correct final size, but also adaptative to adjust to less favorable environmental conditions. Developmental coordination between whole-organism and the brain is particularly important, as the brain is a critical organ with little adaptability. Brain growth mainly depends on neural stem cell (NSC) proliferation to generate differentiated neural cells, it is however unclear how organism developmental progression is coordinated with NSCs. Here we demonstrate that the steroid hormone ecdysone plays a multi-step, stage specific role in regulating Drosophila NSCs, the neuroblasts. We used animals that are unable to synthesize ecdysone, to show that the developmental milestone called “critical weight peak”, the peak that informs the body has reached minimum viable weight to survive metamorphosis, acts a checkpoint necessary to set neuroblast cell cycle pace during larval neurogenesis. The peaks of ecdysone that occur post-critical weight are no longer required to maintain neuroblast division rate. We additionally show that in a second stage, at the onset of pupariation, ecdysone is instead required to trigger neuroblast's proliferation exit and consequently the end of neurogenesis. We demonstrate that, without this signal from ecdysone, neuroblasts lose their ability to exit proliferation. Interestingly, although these neuroblasts proliferate for a longer period, the number of differentiated neurons is smaller compared to wild-type brains, suggesting a role for ecdysone in neuron maintenance. Our study provides insights into how neural stem cells coordinate their division rate with the pace of body growth, identifying a novel coordination mechanism between animal development and NSC proliferation.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)iNOVA4Health - pólo NMSRUNOliveira, Andreia C.Homem, Catarina C.F.2023-10-02T22:18:49Z2023-112023-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/158603eng0012-1606PURE: 72963015https://doi.org/10.1016/j.ydbio.2023.08.001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:08Zoai:run.unl.pt:10362/158603Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:13.725390Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development |
title |
Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development |
spellingShingle |
Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development Oliveira, Andreia C. Brain Drosophila Ecdysone Neuroblast Proliferation Molecular Biology Developmental Biology Cell Biology |
title_short |
Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development |
title_full |
Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development |
title_fullStr |
Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development |
title_full_unstemmed |
Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development |
title_sort |
Opposing effects of ecdysone signaling regulate neuroblast proliferation to ensure coordination of brain and organism development |
author |
Oliveira, Andreia C. |
author_facet |
Oliveira, Andreia C. Homem, Catarina C.F. |
author_role |
author |
author2 |
Homem, Catarina C.F. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) iNOVA4Health - pólo NMS RUN |
dc.contributor.author.fl_str_mv |
Oliveira, Andreia C. Homem, Catarina C.F. |
dc.subject.por.fl_str_mv |
Brain Drosophila Ecdysone Neuroblast Proliferation Molecular Biology Developmental Biology Cell Biology |
topic |
Brain Drosophila Ecdysone Neuroblast Proliferation Molecular Biology Developmental Biology Cell Biology |
description |
Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme ( H2020-ERC-2017-STG-GA 759853 -StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute ( HHMI-208581/Z/17/Z -Metabolic Reg SC fate); EMBO Installation grant ( H2020-EMBO-3311/2017/G2017 ); by Fundação para a Ciência e Tecnologia ( PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health — UIDB/04462/2020 and UIDP/04462/2020 , and by the Associated Laboratory LS4FUTURE ( LA/P/0087/2020 ), two programs financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência , Tecnologia e Ensino Superior . The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170 . Funding Information: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat); by Wellcome Trust and Howard Hughes Medical Institute (HHMI-208581/Z/17/Z-Metabolic Reg SC fate); EMBO Installation grant (H2020-EMBO-3311/2017/G2017); by Fundação para a Ciência e Tecnologia (PTDC/BIA-BID/0681/2021 IF/01265/2014/CP1252/CT0004 and PD/BD/136895/2018 to AO). Nova Medical School was funded by iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), two programs financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior. The Fly facility was funded by CONGENTO LISBOA-01-0145-FEDER- 022170. Publisher Copyright: © 2023 The Authors |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-02T22:18:49Z 2023-11 2023-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/158603 |
url |
http://hdl.handle.net/10362/158603 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0012-1606 PURE: 72963015 https://doi.org/10.1016/j.ydbio.2023.08.001 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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15 application/pdf |
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