Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila

Detalhes bibliográficos
Autor(a) principal: Silva, Eunice A.B.
Data de Publicação: 2023
Outros Autores: Venda, Ana M., Homem, Catarina C.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158431
Resumo: Funding Information: This work was supported by the European Research Council under the European Union’s Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat); Wellcome Trust and Howard Hughes Medical Institute (HHMI-208581/Z/17/ZMetabolicRegSCfate); EMBO Installation grant (H2020-EMBO-3311/2017/G2017) and Fundaçaõ para a Ciência e a Tecnologia (IF/01265/2014/CP1252/CT0004 and SFRH/BD/135262/2017 Eunice Silva); ‘Inova4Health – UIDB/04462/2020 and UIDP/04462/2020, and the Associated Laboratory LS4FUTURE (LA/P/0087/2020), Fundaçaõ para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior’. E.A.B.S. was partially funded by a Fundaçaõ para a Ciência e a Tecnologia doctoral fellowship under the PGCD-Graduate Program Science for Development (SFRH/BD/135262/ 2017). This research was funded in part by the Wellcome Trust [208581/Z/17/Z]. Open Access funding provided by the European Research Council. Deposited in PMC for immediate release. Publisher Copyright: © 2023. Published by The Company of Biologists Ltd.
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spelling Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in DrosophilaDrosophilaNeuroepitheliaOne-carbon metabolismOptic lobeShmtMolecular BiologyDevelopmental BiologyFunding Information: This work was supported by the European Research Council under the European Union’s Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat); Wellcome Trust and Howard Hughes Medical Institute (HHMI-208581/Z/17/ZMetabolicRegSCfate); EMBO Installation grant (H2020-EMBO-3311/2017/G2017) and Fundaçaõ para a Ciência e a Tecnologia (IF/01265/2014/CP1252/CT0004 and SFRH/BD/135262/2017 Eunice Silva); ‘Inova4Health – UIDB/04462/2020 and UIDP/04462/2020, and the Associated Laboratory LS4FUTURE (LA/P/0087/2020), Fundaçaõ para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior’. E.A.B.S. was partially funded by a Fundaçaõ para a Ciência e a Tecnologia doctoral fellowship under the PGCD-Graduate Program Science for Development (SFRH/BD/135262/ 2017). This research was funded in part by the Wellcome Trust [208581/Z/17/Z]. Open Access funding provided by the European Research Council. Deposited in PMC for immediate release. Publisher Copyright: © 2023. Published by The Company of Biologists Ltd.Cell fate and growth require one-carbon units for the biosynthesis of nucleotides, methylation reactions and redox homeostasis, provided by one-carbon metabolism. Consistently, defects in one-carbon metabolism lead to severe developmental defects, such as neural tube defects. However, the role of this pathway during brain development and in neural stem cell regulation is poorly understood. To better understand the role of one carbon metabolism we focused on the enzyme Serine hydroxymethyl transferase (Shmt), a key factor in the one-carbon cycle, during Drosophila brain development. We show that, although loss of Shmt does not cause obvious defects in the central brain, it leads to severe phenotypes in the optic lobe. The shmt mutants have smaller optic lobe neuroepithelia, partly justified by increased apoptosis. In addition, shmt mutant neuroepithelia have morphological defects, failing to form a lamina furrow, which likely explains the observed absence of lamina neurons. These findings show that one-carbon metabolism is crucial for the normal development of neuroepithelia, and consequently for the generation of neural progenitor cells and neurons. These results propose a mechanistic role for one-carbon during brain development.iNOVA4Health - pólo NMSNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNSilva, Eunice A.B.Venda, Ana M.Homem, Catarina C.F.2023-09-28T22:20:26Z2023-102023-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158431eng0950-1991PURE: 72179653https://doi.org/10.1242/dev.201152info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:49Zoai:run.unl.pt:10362/158431Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:08.148562Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila
title Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila
spellingShingle Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila
Silva, Eunice A.B.
Drosophila
Neuroepithelia
One-carbon metabolism
Optic lobe
Shmt
Molecular Biology
Developmental Biology
title_short Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila
title_full Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila
title_fullStr Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila
title_full_unstemmed Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila
title_sort Serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila
author Silva, Eunice A.B.
author_facet Silva, Eunice A.B.
Venda, Ana M.
Homem, Catarina C.F.
author_role author
author2 Venda, Ana M.
Homem, Catarina C.F.
author2_role author
author
dc.contributor.none.fl_str_mv iNOVA4Health - pólo NMS
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Silva, Eunice A.B.
Venda, Ana M.
Homem, Catarina C.F.
dc.subject.por.fl_str_mv Drosophila
Neuroepithelia
One-carbon metabolism
Optic lobe
Shmt
Molecular Biology
Developmental Biology
topic Drosophila
Neuroepithelia
One-carbon metabolism
Optic lobe
Shmt
Molecular Biology
Developmental Biology
description Funding Information: This work was supported by the European Research Council under the European Union’s Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat); Wellcome Trust and Howard Hughes Medical Institute (HHMI-208581/Z/17/ZMetabolicRegSCfate); EMBO Installation grant (H2020-EMBO-3311/2017/G2017) and Fundaçaõ para a Ciência e a Tecnologia (IF/01265/2014/CP1252/CT0004 and SFRH/BD/135262/2017 Eunice Silva); ‘Inova4Health – UIDB/04462/2020 and UIDP/04462/2020, and the Associated Laboratory LS4FUTURE (LA/P/0087/2020), Fundaçaõ para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior’. E.A.B.S. was partially funded by a Fundaçaõ para a Ciência e a Tecnologia doctoral fellowship under the PGCD-Graduate Program Science for Development (SFRH/BD/135262/ 2017). This research was funded in part by the Wellcome Trust [208581/Z/17/Z]. Open Access funding provided by the European Research Council. Deposited in PMC for immediate release. Publisher Copyright: © 2023. Published by The Company of Biologists Ltd.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-28T22:20:26Z
2023-10
2023-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158431
url http://hdl.handle.net/10362/158431
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0950-1991
PURE: 72179653
https://doi.org/10.1242/dev.201152
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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