Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster)
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522005000200005 |
Resumo: | Differential display of Poly (A+) RNA isolated from zygotic embryos of maritime pine (Pinus pinaster) in 9 stages of development was used to isolate several cDNAs of differentially expressed genes during embryo development. Many cDNA sequences share low similarities to any known DNA or protein sequences but 5 transcripts are predicted to encode homologues of DNA-binding proteins, GTP-binding proteins, lipid transferases and dehydrogenases. A full-length cDNA clone was isolated for a gene (named PpAAI-LTSS 1) whose expression appears to be specific to middle stages of development. The full-length cDNA was also isolated for two clones (named PpRab1 and PpCR1) specific to an early stage of embryo development. |
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Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster)pineembryo developmentgene expressionDifferential display of Poly (A+) RNA isolated from zygotic embryos of maritime pine (Pinus pinaster) in 9 stages of development was used to isolate several cDNAs of differentially expressed genes during embryo development. Many cDNA sequences share low similarities to any known DNA or protein sequences but 5 transcripts are predicted to encode homologues of DNA-binding proteins, GTP-binding proteins, lipid transferases and dehydrogenases. A full-length cDNA clone was isolated for a gene (named PpAAI-LTSS 1) whose expression appears to be specific to middle stages of development. The full-length cDNA was also isolated for two clones (named PpRab1 and PpCR1) specific to an early stage of embryo development.Unidade de Silvicultura e Produtos Florestais2005-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522005000200005Silva Lusitana v.13 n.2 2005reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522005000200005Gonçalves,SóniaCairney,JohnOliveira,MargaridaMiguel,Céliainfo:eu-repo/semantics/openAccess2024-02-06T16:59:08Zoai:scielo:S0870-63522005000200005Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:15:34.628085Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster) |
title |
Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster) |
spellingShingle |
Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster) Gonçalves,Sónia pine embryo development gene expression |
title_short |
Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster) |
title_full |
Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster) |
title_fullStr |
Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster) |
title_full_unstemmed |
Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster) |
title_sort |
Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster) |
author |
Gonçalves,Sónia |
author_facet |
Gonçalves,Sónia Cairney,John Oliveira,Margarida Miguel,Célia |
author_role |
author |
author2 |
Cairney,John Oliveira,Margarida Miguel,Célia |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Gonçalves,Sónia Cairney,John Oliveira,Margarida Miguel,Célia |
dc.subject.por.fl_str_mv |
pine embryo development gene expression |
topic |
pine embryo development gene expression |
description |
Differential display of Poly (A+) RNA isolated from zygotic embryos of maritime pine (Pinus pinaster) in 9 stages of development was used to isolate several cDNAs of differentially expressed genes during embryo development. Many cDNA sequences share low similarities to any known DNA or protein sequences but 5 transcripts are predicted to encode homologues of DNA-binding proteins, GTP-binding proteins, lipid transferases and dehydrogenases. A full-length cDNA clone was isolated for a gene (named PpAAI-LTSS 1) whose expression appears to be specific to middle stages of development. The full-length cDNA was also isolated for two clones (named PpRab1 and PpCR1) specific to an early stage of embryo development. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522005000200005 |
url |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522005000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522005000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Unidade de Silvicultura e Produtos Florestais |
publisher.none.fl_str_mv |
Unidade de Silvicultura e Produtos Florestais |
dc.source.none.fl_str_mv |
Silva Lusitana v.13 n.2 2005 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137255251509248 |