Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated threshold
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31881 |
Resumo: | A standardized image analysis method has been developed permitting determination of the number of yeast flocs and their size distribution. The method includes image grabbing, image enhancement, automatic determination of the appropriate threshold, curve fitting of the areahistogram, determination of the mean single floc area and its standard deviation, and floc counting. The extension of the method to other applications is immediate and straightforward. Two Saccharomyces cerevisiae floc Populations (with ages of 48 and 72 h) were analyzed. The results showed a variation around the mean of 9%-12% for the single floc mean area, 6%-7% for the number of single flocs, and 5%-6% for the total number of flocs. Aggregates of two flocs (doublets) and three flocs (triplets) were enumerated. The correctness of the method was checked by analyzing the parameters of interest as a function of the threshold. The constant correlation between the parameters and the threshold showed the validity and consistency of the method. (c) 1996 John Wiley & Sons, Inc. |
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Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated thresholdimage analysisSaccharomyces cerevisiae flocflee countingfloc countingScience & TechnologyA standardized image analysis method has been developed permitting determination of the number of yeast flocs and their size distribution. The method includes image grabbing, image enhancement, automatic determination of the appropriate threshold, curve fitting of the areahistogram, determination of the mean single floc area and its standard deviation, and floc counting. The extension of the method to other applications is immediate and straightforward. Two Saccharomyces cerevisiae floc Populations (with ages of 48 and 72 h) were analyzed. The results showed a variation around the mean of 9%-12% for the single floc mean area, 6%-7% for the number of single flocs, and 5%-6% for the total number of flocs. Aggregates of two flocs (doublets) and three flocs (triplets) were enumerated. The correctness of the method was checked by analyzing the parameters of interest as a function of the threshold. The constant correlation between the parameters and the threshold showed the validity and consistency of the method. (c) 1996 John Wiley & Sons, Inc.WileyUniversidade do MinhoVicente, A. A.Meinders, J.Teixeira, J. A.19961996-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31881eng0006-35921097-029010.1002/bit.260510602http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0290info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:27:42Zoai:repositorium.sdum.uminho.pt:1822/31881Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:21.979984Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated threshold |
title |
Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated threshold |
spellingShingle |
Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated threshold Vicente, A. A. image analysis Saccharomyces cerevisiae floc flee counting floc counting Science & Technology |
title_short |
Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated threshold |
title_full |
Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated threshold |
title_fullStr |
Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated threshold |
title_full_unstemmed |
Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated threshold |
title_sort |
Sizing and counting of Saccharomyces cerevisiae floc populations by image analysis, using an automatically calculated threshold |
author |
Vicente, A. A. |
author_facet |
Vicente, A. A. Meinders, J. Teixeira, J. A. |
author_role |
author |
author2 |
Meinders, J. Teixeira, J. A. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Vicente, A. A. Meinders, J. Teixeira, J. A. |
dc.subject.por.fl_str_mv |
image analysis Saccharomyces cerevisiae floc flee counting floc counting Science & Technology |
topic |
image analysis Saccharomyces cerevisiae floc flee counting floc counting Science & Technology |
description |
A standardized image analysis method has been developed permitting determination of the number of yeast flocs and their size distribution. The method includes image grabbing, image enhancement, automatic determination of the appropriate threshold, curve fitting of the areahistogram, determination of the mean single floc area and its standard deviation, and floc counting. The extension of the method to other applications is immediate and straightforward. Two Saccharomyces cerevisiae floc Populations (with ages of 48 and 72 h) were analyzed. The results showed a variation around the mean of 9%-12% for the single floc mean area, 6%-7% for the number of single flocs, and 5%-6% for the total number of flocs. Aggregates of two flocs (doublets) and three flocs (triplets) were enumerated. The correctness of the method was checked by analyzing the parameters of interest as a function of the threshold. The constant correlation between the parameters and the threshold showed the validity and consistency of the method. (c) 1996 John Wiley & Sons, Inc. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996 1996-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31881 |
url |
http://hdl.handle.net/1822/31881 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0006-3592 1097-0290 10.1002/bit.260510602 http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0290 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132693655453696 |