The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells

Detalhes bibliográficos
Autor(a) principal: Bugalhão, Joana N.
Data de Publicação: 2022
Outros Autores: Luís, Maria P., Pereira, Inês Serrano, da Cunha, Maria, Pais, Sara V., Mota, Luís Jaime
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/134356
Resumo: PTDC/BIA-MIC/28503/2017 PTDC/IMI-MIC/1300/2014 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 PD/BD/128214/2016 PD/BD/52210/2013 PD/00133/2012 SFRH/BD/129756/2017 SFRH/BD/144284/2019
id RCAP_fa9ba1ed44c63e77e7aa9e20ba5b4dd6
oai_identifier_str oai:run.unl.pt:10362/134356
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cellsPTDC/BIA-MIC/28503/2017 PTDC/IMI-MIC/1300/2014 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 PD/BD/128214/2016 PD/BD/52210/2013 PD/00133/2012 SFRH/BD/129756/2017 SFRH/BD/144284/2019Chlamydia trachomatis causes genital and ocular infections in humans. This bacterial pathogen multiplies exclusively within host cells in a characteristic vacuole (inclusion) and delivers proteins such as inclusion membrane proteins (Incs) into the host cell. Here, we identified CT006 as a novel C. trachomatis protein that when expressed ectopically eukaryotic cells can associate with lipid droplets (LDs). A screen using Saccharomyces cerevisiae identified two Incs causing vacuolar protein sorting defects and seven Incs showing tropism for eukaryotic organelles. Ectopic expression in yeast and mammalian cells of genes encoding different fragments of CT006 revealed tropism for the endoplasmic reticulum and LDs. We identified a LD-targeting region within the first 88 amino acid residues of CT006, and positively charged residues important for this targeting. Comparing with the parental wild-type strain, cells infected by a newly generated C. trachomatis strain overproducing CT006 with a double hemagglutinin tag showed a slight increase in the area occupied by LDs within the inclusion region. However, we could not correlate this effect with the LD-targeting regions within CT006. We further showed that both the amino and carboxy-terminal regions of CT006, flanking the Inc-characteristic bilobed hydrophobic domain, are exposed to the host cell cytosol during C. trachomatis infection, supporting their availability to interact with host cell targets. Altogether, our data suggest that CT006 might participate in the interaction of LDs with C. trachomatis inclusions.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNBugalhão, Joana N.Luís, Maria P.Pereira, Inês Serranoda Cunha, MariaPais, Sara V.Mota, Luís Jaime2022-03-11T23:23:22Z2022-022022-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/134356eng1932-6203PURE: 42072364https://doi.org/10.1371/journal.pone.0264292info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:12:50Zoai:run.unl.pt:10362/134356Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:48:04.879717Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells
title The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells
spellingShingle The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells
Bugalhão, Joana N.
title_short The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells
title_full The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells
title_fullStr The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells
title_full_unstemmed The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells
title_sort The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells
author Bugalhão, Joana N.
author_facet Bugalhão, Joana N.
Luís, Maria P.
Pereira, Inês Serrano
da Cunha, Maria
Pais, Sara V.
Mota, Luís Jaime
author_role author
author2 Luís, Maria P.
Pereira, Inês Serrano
da Cunha, Maria
Pais, Sara V.
Mota, Luís Jaime
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Bugalhão, Joana N.
Luís, Maria P.
Pereira, Inês Serrano
da Cunha, Maria
Pais, Sara V.
Mota, Luís Jaime
description PTDC/BIA-MIC/28503/2017 PTDC/IMI-MIC/1300/2014 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 PD/BD/128214/2016 PD/BD/52210/2013 PD/00133/2012 SFRH/BD/129756/2017 SFRH/BD/144284/2019
publishDate 2022
dc.date.none.fl_str_mv 2022-03-11T23:23:22Z
2022-02
2022-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/134356
url http://hdl.handle.net/10362/134356
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 42072364
https://doi.org/10.1371/journal.pone.0264292
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138082406006784

Registros relacionados