The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/134356 |
Resumo: | PTDC/BIA-MIC/28503/2017 PTDC/IMI-MIC/1300/2014 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 PD/BD/128214/2016 PD/BD/52210/2013 PD/00133/2012 SFRH/BD/129756/2017 SFRH/BD/144284/2019 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cellsPTDC/BIA-MIC/28503/2017 PTDC/IMI-MIC/1300/2014 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 PD/BD/128214/2016 PD/BD/52210/2013 PD/00133/2012 SFRH/BD/129756/2017 SFRH/BD/144284/2019Chlamydia trachomatis causes genital and ocular infections in humans. This bacterial pathogen multiplies exclusively within host cells in a characteristic vacuole (inclusion) and delivers proteins such as inclusion membrane proteins (Incs) into the host cell. Here, we identified CT006 as a novel C. trachomatis protein that when expressed ectopically eukaryotic cells can associate with lipid droplets (LDs). A screen using Saccharomyces cerevisiae identified two Incs causing vacuolar protein sorting defects and seven Incs showing tropism for eukaryotic organelles. Ectopic expression in yeast and mammalian cells of genes encoding different fragments of CT006 revealed tropism for the endoplasmic reticulum and LDs. We identified a LD-targeting region within the first 88 amino acid residues of CT006, and positively charged residues important for this targeting. Comparing with the parental wild-type strain, cells infected by a newly generated C. trachomatis strain overproducing CT006 with a double hemagglutinin tag showed a slight increase in the area occupied by LDs within the inclusion region. However, we could not correlate this effect with the LD-targeting regions within CT006. We further showed that both the amino and carboxy-terminal regions of CT006, flanking the Inc-characteristic bilobed hydrophobic domain, are exposed to the host cell cytosol during C. trachomatis infection, supporting their availability to interact with host cell targets. Altogether, our data suggest that CT006 might participate in the interaction of LDs with C. trachomatis inclusions.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNBugalhão, Joana N.Luís, Maria P.Pereira, Inês Serranoda Cunha, MariaPais, Sara V.Mota, Luís Jaime2022-03-11T23:23:22Z2022-022022-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/134356eng1932-6203PURE: 42072364https://doi.org/10.1371/journal.pone.0264292info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:12:50Zoai:run.unl.pt:10362/134356Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:48:04.879717Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells |
title |
The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells |
spellingShingle |
The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells Bugalhão, Joana N. |
title_short |
The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells |
title_full |
The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells |
title_fullStr |
The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells |
title_full_unstemmed |
The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells |
title_sort |
The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells |
author |
Bugalhão, Joana N. |
author_facet |
Bugalhão, Joana N. Luís, Maria P. Pereira, Inês Serrano da Cunha, Maria Pais, Sara V. Mota, Luís Jaime |
author_role |
author |
author2 |
Luís, Maria P. Pereira, Inês Serrano da Cunha, Maria Pais, Sara V. Mota, Luís Jaime |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida RUN |
dc.contributor.author.fl_str_mv |
Bugalhão, Joana N. Luís, Maria P. Pereira, Inês Serrano da Cunha, Maria Pais, Sara V. Mota, Luís Jaime |
description |
PTDC/BIA-MIC/28503/2017 PTDC/IMI-MIC/1300/2014 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 PD/BD/128214/2016 PD/BD/52210/2013 PD/00133/2012 SFRH/BD/129756/2017 SFRH/BD/144284/2019 |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-11T23:23:22Z 2022-02 2022-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/134356 |
url |
http://hdl.handle.net/10362/134356 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 PURE: 42072364 https://doi.org/10.1371/journal.pone.0264292 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799138082406006784 |