Studies of stabilization of native catalase using additives

Detalhes bibliográficos
Autor(a) principal: Costa, Silgia
Data de Publicação: 2002
Outros Autores: Tzanov, Tzanko, Carneiro, Ana Filipa Gonçalves da Costa, Paar, Andreas, Gübitz, Georg M., Paulo, Artur Cavaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/2393
Resumo: Native catalase preparations isolated from Bacillus Sp were formulated with different additives for storage stabilization and better performance at high temperature and pH. The additives studied were: polyethylene glycol, glycerol, BSA, casein, glutaraldehyde, n-butylamine, ethylenediamine, 1.6-diaminohexane, BSA/glutaraldehyde and casein/glutaraldehyde. The glycerol and glutaraldehyde showed the best performance for long-term storage at 30degreesC and neutral pH. No stabilization additives were effective at pH 12, but below that pH the polyethylene glycol and glycerol appeared to be the most appropriate. Amines, polyethylene glycol and glycerol shifted the pH activity maximum of the native catalase toward more alkaline region, while glycerol were the only additive to improve the temperature profile of the enzyme. (C) 2002 Elsevier Science Inc. All rights reserved.
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spelling Studies of stabilization of native catalase using additiveshydrogen peroxidecatalasethermo and alkaline stabilityadditivesScience & TechnologyNative catalase preparations isolated from Bacillus Sp were formulated with different additives for storage stabilization and better performance at high temperature and pH. The additives studied were: polyethylene glycol, glycerol, BSA, casein, glutaraldehyde, n-butylamine, ethylenediamine, 1.6-diaminohexane, BSA/glutaraldehyde and casein/glutaraldehyde. The glycerol and glutaraldehyde showed the best performance for long-term storage at 30degreesC and neutral pH. No stabilization additives were effective at pH 12, but below that pH the polyethylene glycol and glycerol appeared to be the most appropriate. Amines, polyethylene glycol and glycerol shifted the pH activity maximum of the native catalase toward more alkaline region, while glycerol were the only additive to improve the temperature profile of the enzyme. (C) 2002 Elsevier Science Inc. All rights reserved.ElsevierUniversidade do MinhoCosta, SilgiaTzanov, TzankoCarneiro, Ana Filipa Gonçalves da CostaPaar, AndreasGübitz, Georg M.Paulo, Artur Cavaco2002-032002-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2393eng"Enzyme and microbial technology". ISSN 0141-0229. 30:3 (Mar. 2002) 387–391.0141-022910.1016/S0141-0229(01)00505-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:41:26Zoai:repositorium.sdum.uminho.pt:1822/2393Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:38:24.756115Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Studies of stabilization of native catalase using additives
title Studies of stabilization of native catalase using additives
spellingShingle Studies of stabilization of native catalase using additives
Costa, Silgia
hydrogen peroxide
catalase
thermo and alkaline stability
additives
Science & Technology
title_short Studies of stabilization of native catalase using additives
title_full Studies of stabilization of native catalase using additives
title_fullStr Studies of stabilization of native catalase using additives
title_full_unstemmed Studies of stabilization of native catalase using additives
title_sort Studies of stabilization of native catalase using additives
author Costa, Silgia
author_facet Costa, Silgia
Tzanov, Tzanko
Carneiro, Ana Filipa Gonçalves da Costa
Paar, Andreas
Gübitz, Georg M.
Paulo, Artur Cavaco
author_role author
author2 Tzanov, Tzanko
Carneiro, Ana Filipa Gonçalves da Costa
Paar, Andreas
Gübitz, Georg M.
Paulo, Artur Cavaco
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Costa, Silgia
Tzanov, Tzanko
Carneiro, Ana Filipa Gonçalves da Costa
Paar, Andreas
Gübitz, Georg M.
Paulo, Artur Cavaco
dc.subject.por.fl_str_mv hydrogen peroxide
catalase
thermo and alkaline stability
additives
Science & Technology
topic hydrogen peroxide
catalase
thermo and alkaline stability
additives
Science & Technology
description Native catalase preparations isolated from Bacillus Sp were formulated with different additives for storage stabilization and better performance at high temperature and pH. The additives studied were: polyethylene glycol, glycerol, BSA, casein, glutaraldehyde, n-butylamine, ethylenediamine, 1.6-diaminohexane, BSA/glutaraldehyde and casein/glutaraldehyde. The glycerol and glutaraldehyde showed the best performance for long-term storage at 30degreesC and neutral pH. No stabilization additives were effective at pH 12, but below that pH the polyethylene glycol and glycerol appeared to be the most appropriate. Amines, polyethylene glycol and glycerol shifted the pH activity maximum of the native catalase toward more alkaline region, while glycerol were the only additive to improve the temperature profile of the enzyme. (C) 2002 Elsevier Science Inc. All rights reserved.
publishDate 2002
dc.date.none.fl_str_mv 2002-03
2002-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/2393
url http://hdl.handle.net/1822/2393
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Enzyme and microbial technology". ISSN 0141-0229. 30:3 (Mar. 2002) 387–391.
0141-0229
10.1016/S0141-0229(01)00505-1
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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