Evaluation of canine adipose-derived stem cells in a healthy mice subcutaneous model

• Autor(a) principal: Requicha, J. F.
• Data de Publicação: 2016
• Outros Autores: Carvalho, P. P., Pires, M. A., Dias, M. I., Reis, R. L., Gomes, Manuela E., Viegas, C. A. A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/44326
• topic: Canine adipose-derived stem cells; Mice subcutaneous model; Dog; Xenotransplantation;; Host response; Mice model

Canine adipose-derived stem cells (cASCs) have great interest for cell-based therapies in Veterinary Medicine. As the behaviour of these cells in non-autologous recipients is not deeply characterized, it is mandatory to study them in new animal models previously to canine specie. In this work, cASCs were injected subcutaneously in mice and these cells were detected by immunohistochemistry using vimentin, CD44 and keratin. The local response evaluated by histology did not reveal signals of significant inflammatory reaction neither in the lymph nodes or other organs. This study showed the implantation of cASCs induced a scarce inflammatory response. Hsd:CD1 (ICR) mouse can be proposed as an animal model to study the in vivo behavior of the cASCs and to validate new cASCs-based approaches avoiding or reducing the use of dogs in research.