Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Botanica Brasilica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400017 |
Resumo: | The traditional techniques employed for embedding plant samples are not suitable for the proper processing of small seeds with hard seed coats. Usually, these seeds are broken off from blocks during microtomy, which limits the technical success of this procedure. In this study, Melastomataceae seeds of 101 species were treated prior to embedding in historesin according to three treatments: (1) control, using the standard procedure; (2) fixation and subsequent softening with Franklin solution, glycerin and heating in a water bath; and (3) softening with Franklin solution and subsequent fixation (glycerin and heating in a water bath were also included). For Melastomataceae species, the second treatment provides the best results, and we were able to produce very good sections of the entire seed. Small hard seeds, similar to those found in Melastomataceae, are better embedded when they are softened after subjected to fixation. A combination of softening techniques is necessary to improve the embedding process and to obtain high-quality sections of the embedded samples. In this study, we established a practical, slightly toxic and inexpensive methodology to ensure good preparations for light microscopy that can be applied to a wide range of subjects related to seed science. |
id |
SBB-1_6a33a792eb7a51033826b91293d44ede |
---|---|
oai_identifier_str |
oai:scielo:S0102-33062014000400017 |
network_acronym_str |
SBB-1 |
network_name_str |
Acta Botanica Brasilica |
repository_id_str |
|
spelling |
Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopyAnatomyhistoresin embeddingMelastomataceaesofteningThe traditional techniques employed for embedding plant samples are not suitable for the proper processing of small seeds with hard seed coats. Usually, these seeds are broken off from blocks during microtomy, which limits the technical success of this procedure. In this study, Melastomataceae seeds of 101 species were treated prior to embedding in historesin according to three treatments: (1) control, using the standard procedure; (2) fixation and subsequent softening with Franklin solution, glycerin and heating in a water bath; and (3) softening with Franklin solution and subsequent fixation (glycerin and heating in a water bath were also included). For Melastomataceae species, the second treatment provides the best results, and we were able to produce very good sections of the entire seed. Small hard seeds, similar to those found in Melastomataceae, are better embedded when they are softened after subjected to fixation. A combination of softening techniques is necessary to improve the embedding process and to obtain high-quality sections of the embedded samples. In this study, we established a practical, slightly toxic and inexpensive methodology to ensure good preparations for light microscopy that can be applied to a wide range of subjects related to seed science.Sociedade Botânica do Brasil2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400017Acta Botanica Brasilica v.28 n.4 2014reponame:Acta Botanica Brasilicainstname:Sociedade Botânica do Brasil (SBB)instacron:SBB10.1590/0102-33062014abb3654info:eu-repo/semantics/openAccessRibeiro,Rafaella CardosoOliveira,Denise Maria Tromberteng2015-01-08T00:00:00Zoai:scielo:S0102-33062014000400017Revistahttps://www.scielo.br/j/abb/ONGhttps://old.scielo.br/oai/scielo-oai.phpacta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com1677-941X0102-3306opendoar:2015-01-08T00:00Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)false |
dc.title.none.fl_str_mv |
Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopy |
title |
Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopy |
spellingShingle |
Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopy Ribeiro,Rafaella Cardoso Anatomy historesin embedding Melastomataceae softening |
title_short |
Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopy |
title_full |
Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopy |
title_fullStr |
Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopy |
title_full_unstemmed |
Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopy |
title_sort |
Small and hard seeds: a practical and inexpensive method to improve embedding techniques for light microscopy |
author |
Ribeiro,Rafaella Cardoso |
author_facet |
Ribeiro,Rafaella Cardoso Oliveira,Denise Maria Trombert |
author_role |
author |
author2 |
Oliveira,Denise Maria Trombert |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Ribeiro,Rafaella Cardoso Oliveira,Denise Maria Trombert |
dc.subject.por.fl_str_mv |
Anatomy historesin embedding Melastomataceae softening |
topic |
Anatomy historesin embedding Melastomataceae softening |
description |
The traditional techniques employed for embedding plant samples are not suitable for the proper processing of small seeds with hard seed coats. Usually, these seeds are broken off from blocks during microtomy, which limits the technical success of this procedure. In this study, Melastomataceae seeds of 101 species were treated prior to embedding in historesin according to three treatments: (1) control, using the standard procedure; (2) fixation and subsequent softening with Franklin solution, glycerin and heating in a water bath; and (3) softening with Franklin solution and subsequent fixation (glycerin and heating in a water bath were also included). For Melastomataceae species, the second treatment provides the best results, and we were able to produce very good sections of the entire seed. Small hard seeds, similar to those found in Melastomataceae, are better embedded when they are softened after subjected to fixation. A combination of softening techniques is necessary to improve the embedding process and to obtain high-quality sections of the embedded samples. In this study, we established a practical, slightly toxic and inexpensive methodology to ensure good preparations for light microscopy that can be applied to a wide range of subjects related to seed science. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0102-33062014abb3654 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
dc.source.none.fl_str_mv |
Acta Botanica Brasilica v.28 n.4 2014 reponame:Acta Botanica Brasilica instname:Sociedade Botânica do Brasil (SBB) instacron:SBB |
instname_str |
Sociedade Botânica do Brasil (SBB) |
instacron_str |
SBB |
institution |
SBB |
reponame_str |
Acta Botanica Brasilica |
collection |
Acta Botanica Brasilica |
repository.name.fl_str_mv |
Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB) |
repository.mail.fl_str_mv |
acta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com |
_version_ |
1752126661299535872 |