Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity

Detalhes bibliográficos
Autor(a) principal: Jacobsen,Bruno Barcellos
Data de Publicação: 2017
Outros Autores: Leopoldo,Ana Paula Lima, Cordeiro,Jóctan Pimentel, Campos,Dijon Henrique Salomé de, Nascimento,André Ferreira do, Sugizaki,Mário Mateus, Cicogna,Antônio Carlos, Padovani,Carlos Roberto, Leopoldo,André Soares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos Brasileiros de Cardiologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2017001400432
Resumo: Abstract Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified. Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot. Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins. Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling.
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spelling Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of ObesityRatsObesityDietHight-FatCardiac functionCalciumAdiposityAbstract Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified. Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot. Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins. Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling.Sociedade Brasileira de Cardiologia - SBC2017-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2017001400432Arquivos Brasileiros de Cardiologia v.109 n.5 2017reponame:Arquivos Brasileiros de Cardiologia (Online)instname:Sociedade Brasileira de Cardiologia (SBC)instacron:SBC10.5935/abc.20170151info:eu-repo/semantics/openAccessJacobsen,Bruno BarcellosLeopoldo,Ana Paula LimaCordeiro,Jóctan PimentelCampos,Dijon Henrique Salomé deNascimento,André Ferreira doSugizaki,Mário MateusCicogna,Antônio CarlosPadovani,Carlos RobertoLeopoldo,André Soareseng2017-12-11T00:00:00Zoai:scielo:S0066-782X2017001400432Revistahttp://www.arquivosonline.com.br/https://old.scielo.br/oai/scielo-oai.php||arquivos@cardiol.br1678-41700066-782Xopendoar:2017-12-11T00:00Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC)false
dc.title.none.fl_str_mv Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
spellingShingle Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
Jacobsen,Bruno Barcellos
Rats
Obesity
Diet
Hight-Fat
Cardiac function
Calcium
Adiposity
title_short Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title_full Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title_fullStr Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title_full_unstemmed Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title_sort Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
author Jacobsen,Bruno Barcellos
author_facet Jacobsen,Bruno Barcellos
Leopoldo,Ana Paula Lima
Cordeiro,Jóctan Pimentel
Campos,Dijon Henrique Salomé de
Nascimento,André Ferreira do
Sugizaki,Mário Mateus
Cicogna,Antônio Carlos
Padovani,Carlos Roberto
Leopoldo,André Soares
author_role author
author2 Leopoldo,Ana Paula Lima
Cordeiro,Jóctan Pimentel
Campos,Dijon Henrique Salomé de
Nascimento,André Ferreira do
Sugizaki,Mário Mateus
Cicogna,Antônio Carlos
Padovani,Carlos Roberto
Leopoldo,André Soares
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jacobsen,Bruno Barcellos
Leopoldo,Ana Paula Lima
Cordeiro,Jóctan Pimentel
Campos,Dijon Henrique Salomé de
Nascimento,André Ferreira do
Sugizaki,Mário Mateus
Cicogna,Antônio Carlos
Padovani,Carlos Roberto
Leopoldo,André Soares
dc.subject.por.fl_str_mv Rats
Obesity
Diet
Hight-Fat
Cardiac function
Calcium
Adiposity
topic Rats
Obesity
Diet
Hight-Fat
Cardiac function
Calcium
Adiposity
description Abstract Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified. Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot. Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins. Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.5935/abc.20170151
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia - SBC
publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia - SBC
dc.source.none.fl_str_mv Arquivos Brasileiros de Cardiologia v.109 n.5 2017
reponame:Arquivos Brasileiros de Cardiologia (Online)
instname:Sociedade Brasileira de Cardiologia (SBC)
instacron:SBC
instname_str Sociedade Brasileira de Cardiologia (SBC)
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institution SBC
reponame_str Arquivos Brasileiros de Cardiologia (Online)
collection Arquivos Brasileiros de Cardiologia (Online)
repository.name.fl_str_mv Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC)
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