Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos Brasileiros de Cardiologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2017001400432 |
Resumo: | Abstract Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified. Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot. Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins. Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling. |
id |
SBC-1_71aec6658d4595ffa3d926cd96ed664b |
---|---|
oai_identifier_str |
oai:scielo:S0066-782X2017001400432 |
network_acronym_str |
SBC-1 |
network_name_str |
Arquivos Brasileiros de Cardiologia (Online) |
repository_id_str |
|
spelling |
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of ObesityRatsObesityDietHight-FatCardiac functionCalciumAdiposityAbstract Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified. Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot. Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins. Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling.Sociedade Brasileira de Cardiologia - SBC2017-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2017001400432Arquivos Brasileiros de Cardiologia v.109 n.5 2017reponame:Arquivos Brasileiros de Cardiologia (Online)instname:Sociedade Brasileira de Cardiologia (SBC)instacron:SBC10.5935/abc.20170151info:eu-repo/semantics/openAccessJacobsen,Bruno BarcellosLeopoldo,Ana Paula LimaCordeiro,Jóctan PimentelCampos,Dijon Henrique Salomé deNascimento,André Ferreira doSugizaki,Mário MateusCicogna,Antônio CarlosPadovani,Carlos RobertoLeopoldo,André Soareseng2017-12-11T00:00:00Zoai:scielo:S0066-782X2017001400432Revistahttp://www.arquivosonline.com.br/https://old.scielo.br/oai/scielo-oai.php||arquivos@cardiol.br1678-41700066-782Xopendoar:2017-12-11T00:00Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC)false |
dc.title.none.fl_str_mv |
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity |
title |
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity |
spellingShingle |
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity Jacobsen,Bruno Barcellos Rats Obesity Diet Hight-Fat Cardiac function Calcium Adiposity |
title_short |
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity |
title_full |
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity |
title_fullStr |
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity |
title_full_unstemmed |
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity |
title_sort |
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity |
author |
Jacobsen,Bruno Barcellos |
author_facet |
Jacobsen,Bruno Barcellos Leopoldo,Ana Paula Lima Cordeiro,Jóctan Pimentel Campos,Dijon Henrique Salomé de Nascimento,André Ferreira do Sugizaki,Mário Mateus Cicogna,Antônio Carlos Padovani,Carlos Roberto Leopoldo,André Soares |
author_role |
author |
author2 |
Leopoldo,Ana Paula Lima Cordeiro,Jóctan Pimentel Campos,Dijon Henrique Salomé de Nascimento,André Ferreira do Sugizaki,Mário Mateus Cicogna,Antônio Carlos Padovani,Carlos Roberto Leopoldo,André Soares |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Jacobsen,Bruno Barcellos Leopoldo,Ana Paula Lima Cordeiro,Jóctan Pimentel Campos,Dijon Henrique Salomé de Nascimento,André Ferreira do Sugizaki,Mário Mateus Cicogna,Antônio Carlos Padovani,Carlos Roberto Leopoldo,André Soares |
dc.subject.por.fl_str_mv |
Rats Obesity Diet Hight-Fat Cardiac function Calcium Adiposity |
topic |
Rats Obesity Diet Hight-Fat Cardiac function Calcium Adiposity |
description |
Abstract Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified. Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot. Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins. Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2017001400432 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2017001400432 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/abc.20170151 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Cardiologia - SBC |
publisher.none.fl_str_mv |
Sociedade Brasileira de Cardiologia - SBC |
dc.source.none.fl_str_mv |
Arquivos Brasileiros de Cardiologia v.109 n.5 2017 reponame:Arquivos Brasileiros de Cardiologia (Online) instname:Sociedade Brasileira de Cardiologia (SBC) instacron:SBC |
instname_str |
Sociedade Brasileira de Cardiologia (SBC) |
instacron_str |
SBC |
institution |
SBC |
reponame_str |
Arquivos Brasileiros de Cardiologia (Online) |
collection |
Arquivos Brasileiros de Cardiologia (Online) |
repository.name.fl_str_mv |
Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC) |
repository.mail.fl_str_mv |
||arquivos@cardiol.br |
_version_ |
1752126568090566656 |