Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity

Detalhes bibliográficos
Autor(a) principal: Jacobsen, Bruno Barcellos
Data de Publicação: 2017
Outros Autores: Leopoldo, Ana Paula Lima, Cordeiro, Jóctan Pimentel, de Campos, Dijon Henrique Salomé [UNESP], do Nascimento, André Ferreira, Sugizaki, Mário Mateus, Cicogna, Antônio Carlos [UNESP], Padovani, Carlos Roberto [UNESP], Leopoldo, André Soares
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5935/abc.20170151
http://hdl.handle.net/11449/179450
Resumo: Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling.Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified.Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot.Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins.Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling.
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spelling Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesityPerfis cardíaco, metabólico e molecular de ratos sedentários no momento inicial da obesidadeAdiposityCalciumCardiac functionDietHight-fatObesityRatsBackground: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling.Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified.Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot.Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins.Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling.Fundação Estadual de Amparo à Pesquisa do Estado do Espírito SantoFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal do Espírito Santo (UFES)Departamento de Clínica Médica Faculdade de Medicina UNESPUniversidade Federal de Mato Grosso (UFMT)Departamento de Clínica Médica Faculdade de Medicina UNESPUniversidade Federal do Espírito Santo (UFES)Universidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso (UFMT)Jacobsen, Bruno BarcellosLeopoldo, Ana Paula LimaCordeiro, Jóctan Pimentelde Campos, Dijon Henrique Salomé [UNESP]do Nascimento, André FerreiraSugizaki, Mário MateusCicogna, Antônio Carlos [UNESP]Padovani, Carlos Roberto [UNESP]Leopoldo, André Soares2018-12-11T17:35:14Z2018-12-11T17:35:14Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article432-439application/pdfhttp://dx.doi.org/10.5935/abc.20170151Arquivos Brasileiros de Cardiologia, v. 109, n. 5, p. 432-439, 2017.1678-41700066-782Xhttp://hdl.handle.net/11449/17945010.5935/abc.20170151S0066-782X20170014004322-s2.0-85038445865S0066-782X2017001400432.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporArquivos Brasileiros de Cardiologiainfo:eu-repo/semantics/openAccess2024-08-14T17:23:22Zoai:repositorio.unesp.br:11449/179450Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
Perfis cardíaco, metabólico e molecular de ratos sedentários no momento inicial da obesidade
title Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
spellingShingle Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
Jacobsen, Bruno Barcellos
Adiposity
Calcium
Cardiac function
Diet
Hight-fat
Obesity
Rats
title_short Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
title_full Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
title_fullStr Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
title_full_unstemmed Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
title_sort Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
author Jacobsen, Bruno Barcellos
author_facet Jacobsen, Bruno Barcellos
Leopoldo, Ana Paula Lima
Cordeiro, Jóctan Pimentel
de Campos, Dijon Henrique Salomé [UNESP]
do Nascimento, André Ferreira
Sugizaki, Mário Mateus
Cicogna, Antônio Carlos [UNESP]
Padovani, Carlos Roberto [UNESP]
Leopoldo, André Soares
author_role author
author2 Leopoldo, Ana Paula Lima
Cordeiro, Jóctan Pimentel
de Campos, Dijon Henrique Salomé [UNESP]
do Nascimento, André Ferreira
Sugizaki, Mário Mateus
Cicogna, Antônio Carlos [UNESP]
Padovani, Carlos Roberto [UNESP]
Leopoldo, André Soares
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Espírito Santo (UFES)
Universidade Estadual Paulista (Unesp)
Universidade Federal de Mato Grosso (UFMT)
dc.contributor.author.fl_str_mv Jacobsen, Bruno Barcellos
Leopoldo, Ana Paula Lima
Cordeiro, Jóctan Pimentel
de Campos, Dijon Henrique Salomé [UNESP]
do Nascimento, André Ferreira
Sugizaki, Mário Mateus
Cicogna, Antônio Carlos [UNESP]
Padovani, Carlos Roberto [UNESP]
Leopoldo, André Soares
dc.subject.por.fl_str_mv Adiposity
Calcium
Cardiac function
Diet
Hight-fat
Obesity
Rats
topic Adiposity
Calcium
Cardiac function
Diet
Hight-fat
Obesity
Rats
description Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling.Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified.Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot.Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins.Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-01
2018-12-11T17:35:14Z
2018-12-11T17:35:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5935/abc.20170151
Arquivos Brasileiros de Cardiologia, v. 109, n. 5, p. 432-439, 2017.
1678-4170
0066-782X
http://hdl.handle.net/11449/179450
10.5935/abc.20170151
S0066-782X2017001400432
2-s2.0-85038445865
S0066-782X2017001400432.pdf
url http://dx.doi.org/10.5935/abc.20170151
http://hdl.handle.net/11449/179450
identifier_str_mv Arquivos Brasileiros de Cardiologia, v. 109, n. 5, p. 432-439, 2017.
1678-4170
0066-782X
10.5935/abc.20170151
S0066-782X2017001400432
2-s2.0-85038445865
S0066-782X2017001400432.pdf
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Arquivos Brasileiros de Cardiologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 432-439
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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