Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5935/abc.20170151 http://hdl.handle.net/11449/179450 |
Resumo: | Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling.Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified.Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot.Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins.Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling. |
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Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesityPerfis cardíaco, metabólico e molecular de ratos sedentários no momento inicial da obesidadeAdiposityCalciumCardiac functionDietHight-fatObesityRatsBackground: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling.Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified.Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot.Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins.Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling.Fundação Estadual de Amparo à Pesquisa do Estado do Espírito SantoFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal do Espírito Santo (UFES)Departamento de Clínica Médica Faculdade de Medicina UNESPUniversidade Federal de Mato Grosso (UFMT)Departamento de Clínica Médica Faculdade de Medicina UNESPUniversidade Federal do Espírito Santo (UFES)Universidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso (UFMT)Jacobsen, Bruno BarcellosLeopoldo, Ana Paula LimaCordeiro, Jóctan Pimentelde Campos, Dijon Henrique Salomé [UNESP]do Nascimento, André FerreiraSugizaki, Mário MateusCicogna, Antônio Carlos [UNESP]Padovani, Carlos Roberto [UNESP]Leopoldo, André Soares2018-12-11T17:35:14Z2018-12-11T17:35:14Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article432-439application/pdfhttp://dx.doi.org/10.5935/abc.20170151Arquivos Brasileiros de Cardiologia, v. 109, n. 5, p. 432-439, 2017.1678-41700066-782Xhttp://hdl.handle.net/11449/17945010.5935/abc.20170151S0066-782X20170014004322-s2.0-85038445865S0066-782X2017001400432.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporArquivos Brasileiros de Cardiologiainfo:eu-repo/semantics/openAccess2024-08-14T17:23:22Zoai:repositorio.unesp.br:11449/179450Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity Perfis cardíaco, metabólico e molecular de ratos sedentários no momento inicial da obesidade |
title |
Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity |
spellingShingle |
Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity Jacobsen, Bruno Barcellos Adiposity Calcium Cardiac function Diet Hight-fat Obesity Rats |
title_short |
Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity |
title_full |
Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity |
title_fullStr |
Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity |
title_full_unstemmed |
Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity |
title_sort |
Cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity |
author |
Jacobsen, Bruno Barcellos |
author_facet |
Jacobsen, Bruno Barcellos Leopoldo, Ana Paula Lima Cordeiro, Jóctan Pimentel de Campos, Dijon Henrique Salomé [UNESP] do Nascimento, André Ferreira Sugizaki, Mário Mateus Cicogna, Antônio Carlos [UNESP] Padovani, Carlos Roberto [UNESP] Leopoldo, André Soares |
author_role |
author |
author2 |
Leopoldo, Ana Paula Lima Cordeiro, Jóctan Pimentel de Campos, Dijon Henrique Salomé [UNESP] do Nascimento, André Ferreira Sugizaki, Mário Mateus Cicogna, Antônio Carlos [UNESP] Padovani, Carlos Roberto [UNESP] Leopoldo, André Soares |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Espírito Santo (UFES) Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso (UFMT) |
dc.contributor.author.fl_str_mv |
Jacobsen, Bruno Barcellos Leopoldo, Ana Paula Lima Cordeiro, Jóctan Pimentel de Campos, Dijon Henrique Salomé [UNESP] do Nascimento, André Ferreira Sugizaki, Mário Mateus Cicogna, Antônio Carlos [UNESP] Padovani, Carlos Roberto [UNESP] Leopoldo, André Soares |
dc.subject.por.fl_str_mv |
Adiposity Calcium Cardiac function Diet Hight-fat Obesity Rats |
topic |
Adiposity Calcium Cardiac function Diet Hight-fat Obesity Rats |
description |
Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling.Objective: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca2+ handling in short-term exposure to obesity was verified.Methods: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca2+ handling proteins were determined by Western Blot.Results: The initial moment of obesity occurred at the 3rd week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm2/s and HF: 68.0 ± 17.0 g/mm2/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca2+ handling proteins.Conclusion: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca2+ handling. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-12-11T17:35:14Z 2018-12-11T17:35:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5935/abc.20170151 Arquivos Brasileiros de Cardiologia, v. 109, n. 5, p. 432-439, 2017. 1678-4170 0066-782X http://hdl.handle.net/11449/179450 10.5935/abc.20170151 S0066-782X2017001400432 2-s2.0-85038445865 S0066-782X2017001400432.pdf |
url |
http://dx.doi.org/10.5935/abc.20170151 http://hdl.handle.net/11449/179450 |
identifier_str_mv |
Arquivos Brasileiros de Cardiologia, v. 109, n. 5, p. 432-439, 2017. 1678-4170 0066-782X 10.5935/abc.20170151 S0066-782X2017001400432 2-s2.0-85038445865 S0066-782X2017001400432.pdf |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Cardiologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
432-439 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128163085025280 |