Development of experimental in vitro burn model

Detalhes bibliográficos
Autor(a) principal: Fernandes,Ana Carolina Morais
Data de Publicação: 2014
Outros Autores: França,Jerônimo Pereira de, Gaiba,Silvana, Aloise,Antonio Carlos, Oliveira,Andrea Fernandes de, Moraes,Andrea Aparecida de Fátima Souza, França,Lucimar Pereira de, Ferreira,Lydia Masako
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400005
Resumo: PURPOSE: To propose an experimental burn model in NIH-3T3 cell line.METHODS:Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS:The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION:The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.
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spelling Development of experimental in vitro burn modelBurnfibroblastcell line NIH-3T3microwaveMTTConfocal microscopyPURPOSE: To propose an experimental burn model in NIH-3T3 cell line.METHODS:Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS:The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION:The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400005Acta Cirúrgica Brasileira v.29 suppl.2 2014reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502014001400004info:eu-repo/semantics/openAccessFernandes,Ana Carolina MoraisFrança,Jerônimo Pereira deGaiba,SilvanaAloise,Antonio CarlosOliveira,Andrea Fernandes deMoraes,Andrea Aparecida de Fátima SouzaFrança,Lucimar Pereira deFerreira,Lydia Masakoeng2015-10-08T00:00:00Zoai:scielo:S0102-86502014001400005Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-10-08T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Development of experimental in vitro burn model
title Development of experimental in vitro burn model
spellingShingle Development of experimental in vitro burn model
Fernandes,Ana Carolina Morais
Burn
fibroblast
cell line NIH-3T3
microwave
MTT
Confocal microscopy
title_short Development of experimental in vitro burn model
title_full Development of experimental in vitro burn model
title_fullStr Development of experimental in vitro burn model
title_full_unstemmed Development of experimental in vitro burn model
title_sort Development of experimental in vitro burn model
author Fernandes,Ana Carolina Morais
author_facet Fernandes,Ana Carolina Morais
França,Jerônimo Pereira de
Gaiba,Silvana
Aloise,Antonio Carlos
Oliveira,Andrea Fernandes de
Moraes,Andrea Aparecida de Fátima Souza
França,Lucimar Pereira de
Ferreira,Lydia Masako
author_role author
author2 França,Jerônimo Pereira de
Gaiba,Silvana
Aloise,Antonio Carlos
Oliveira,Andrea Fernandes de
Moraes,Andrea Aparecida de Fátima Souza
França,Lucimar Pereira de
Ferreira,Lydia Masako
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes,Ana Carolina Morais
França,Jerônimo Pereira de
Gaiba,Silvana
Aloise,Antonio Carlos
Oliveira,Andrea Fernandes de
Moraes,Andrea Aparecida de Fátima Souza
França,Lucimar Pereira de
Ferreira,Lydia Masako
dc.subject.por.fl_str_mv Burn
fibroblast
cell line NIH-3T3
microwave
MTT
Confocal microscopy
topic Burn
fibroblast
cell line NIH-3T3
microwave
MTT
Confocal microscopy
description PURPOSE: To propose an experimental burn model in NIH-3T3 cell line.METHODS:Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS:The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION:The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502014001400004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.29 suppl.2 2014
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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