Development of experimental in vitro burn model

Detalhes bibliográficos
Autor(a) principal: Fernandes, Ana Carolina Morais
Data de Publicação: 2014
Outros Autores: França, Jerônimo Pereira de [UNIFESP], Gaiba, Silvana, Aloise, Antonio Carlos [UNIFESP], Oliveira, Andrea Fernandes de, Moraes, Andrea Aparecida Fátima Souza [UNIFESP], França, Lucimar Pereira de, Ferreira, Lydia Masako [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502014001400004
http://repositorio.unifesp.br/handle/11600/8144
Resumo: PURPOSE:To propose an experimental burn model in NIH-3T3 cell line.METHODS: Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS: The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION: The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.
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spelling Development of experimental in vitro burn modelBurnfibroblastcell line NIH-3T3microwaveMTTConfocal microscopyPURPOSE:To propose an experimental burn model in NIH-3T3 cell line.METHODS: Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS: The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION: The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Santa Cruz Department of Biological SciencesUNIFESPUNIFESPSciELOConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual de Santa Cruz Department of Biological SciencesFernandes, Ana Carolina MoraisFrança, Jerônimo Pereira de [UNIFESP]Gaiba, SilvanaAloise, Antonio Carlos [UNIFESP]Oliveira, Andrea Fernandes deMoraes, Andrea Aparecida Fátima Souza [UNIFESP]França, Lucimar Pereira deFerreira, Lydia Masako [UNIFESP]2015-06-14T13:46:49Z2015-06-14T13:46:49Z2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15-20application/pdfhttp://dx.doi.org/10.1590/S0102-86502014001400004Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 15-20, 2014.10.1590/S0102-86502014001400004S0102-86502014001400005.pdf0102-8650S0102-86502014001400005http://repositorio.unifesp.br/handle/11600/8144WOS:000342065300005engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T20:22:04Zoai:repositorio.unifesp.br/:11600/8144Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T20:22:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Development of experimental in vitro burn model
title Development of experimental in vitro burn model
spellingShingle Development of experimental in vitro burn model
Fernandes, Ana Carolina Morais
Burn
fibroblast
cell line NIH-3T3
microwave
MTT
Confocal microscopy
title_short Development of experimental in vitro burn model
title_full Development of experimental in vitro burn model
title_fullStr Development of experimental in vitro burn model
title_full_unstemmed Development of experimental in vitro burn model
title_sort Development of experimental in vitro burn model
author Fernandes, Ana Carolina Morais
author_facet Fernandes, Ana Carolina Morais
França, Jerônimo Pereira de [UNIFESP]
Gaiba, Silvana
Aloise, Antonio Carlos [UNIFESP]
Oliveira, Andrea Fernandes de
Moraes, Andrea Aparecida Fátima Souza [UNIFESP]
França, Lucimar Pereira de
Ferreira, Lydia Masako [UNIFESP]
author_role author
author2 França, Jerônimo Pereira de [UNIFESP]
Gaiba, Silvana
Aloise, Antonio Carlos [UNIFESP]
Oliveira, Andrea Fernandes de
Moraes, Andrea Aparecida Fátima Souza [UNIFESP]
França, Lucimar Pereira de
Ferreira, Lydia Masako [UNIFESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Santa Cruz Department of Biological Sciences
dc.contributor.author.fl_str_mv Fernandes, Ana Carolina Morais
França, Jerônimo Pereira de [UNIFESP]
Gaiba, Silvana
Aloise, Antonio Carlos [UNIFESP]
Oliveira, Andrea Fernandes de
Moraes, Andrea Aparecida Fátima Souza [UNIFESP]
França, Lucimar Pereira de
Ferreira, Lydia Masako [UNIFESP]
dc.subject.por.fl_str_mv Burn
fibroblast
cell line NIH-3T3
microwave
MTT
Confocal microscopy
topic Burn
fibroblast
cell line NIH-3T3
microwave
MTT
Confocal microscopy
description PURPOSE:To propose an experimental burn model in NIH-3T3 cell line.METHODS: Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS: The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION: The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2015-06-14T13:46:49Z
2015-06-14T13:46:49Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502014001400004
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 15-20, 2014.
10.1590/S0102-86502014001400004
S0102-86502014001400005.pdf
0102-8650
S0102-86502014001400005
http://repositorio.unifesp.br/handle/11600/8144
WOS:000342065300005
url http://dx.doi.org/10.1590/S0102-86502014001400004
http://repositorio.unifesp.br/handle/11600/8144
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 15-20, 2014.
10.1590/S0102-86502014001400004
S0102-86502014001400005.pdf
0102-8650
S0102-86502014001400005
WOS:000342065300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15-20
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268427177558016

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