Experimental rat lung tumor model with intrabronchial tumor cell implantation

Detalhes bibliográficos
Autor(a) principal: Gomes Neto,Antero
Data de Publicação: 2008
Outros Autores: Simão,Antônio Felipe Leite, Miranda,Samuel de Paula, Mourão,Lívia Talita Cajaseiras, Bezerra,Nilfácio Prado, Almeida,Paulo Roberto Carvalho de, Ribeiro,Ronaldo de Albuquerque
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000100014
Resumo: PURPOSE: The objective of this study was to develop a rat lung tumor model for anticancer drug testing. METHODS: Sixty-two female Wistar rats weighing 208 ± 20 g were anesthetized intraperitoneally with 2.5% tribromoethanol (1 ml/100 g live weight), tracheotomized and intubated with an ultrafine catheter for inoculation with Walker's tumor cells. In the first step of the experiment, a technique was established for intrabronchial implantation of 10(5) to 5×10(5) tumor cells, and the tumor take rate was determined. The second stage consisted of determining tumor volume, correlating findings from high-resolution computed tomography (HRCT) with findings from necropsia and determining time of survival. RESULTS: The tumor take rate was 94.7% for implants with 4×10(5) tumor cells, HRCT and necropsia findings matched closely (r=0.953; p<0.0001), the median time of survival was 11 days, and surgical mortality was 4.8%. CONCLUSION: The present rat lung tumor model was shown to be feasible: the take rate was high, surgical mortality was negligible and the procedure was simple to perform and easily reproduced. HRCT was found to be a highly accurate tool for tumor diagnosis, localization and measurement and may be recommended for monitoring tumor growth in this model.
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spelling Experimental rat lung tumor model with intrabronchial tumor cell implantationLung neoplasmsWalker-256 carcinomaratsPURPOSE: The objective of this study was to develop a rat lung tumor model for anticancer drug testing. METHODS: Sixty-two female Wistar rats weighing 208 ± 20 g were anesthetized intraperitoneally with 2.5% tribromoethanol (1 ml/100 g live weight), tracheotomized and intubated with an ultrafine catheter for inoculation with Walker's tumor cells. In the first step of the experiment, a technique was established for intrabronchial implantation of 10(5) to 5×10(5) tumor cells, and the tumor take rate was determined. The second stage consisted of determining tumor volume, correlating findings from high-resolution computed tomography (HRCT) with findings from necropsia and determining time of survival. RESULTS: The tumor take rate was 94.7% for implants with 4×10(5) tumor cells, HRCT and necropsia findings matched closely (r=0.953; p<0.0001), the median time of survival was 11 days, and surgical mortality was 4.8%. CONCLUSION: The present rat lung tumor model was shown to be feasible: the take rate was high, surgical mortality was negligible and the procedure was simple to perform and easily reproduced. HRCT was found to be a highly accurate tool for tumor diagnosis, localization and measurement and may be recommended for monitoring tumor growth in this model.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2008-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000100014Acta Cirúrgica Brasileira v.23 n.1 2008reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502008000100014info:eu-repo/semantics/openAccessGomes Neto,AnteroSimão,Antônio Felipe LeiteMiranda,Samuel de PaulaMourão,Lívia Talita CajaseirasBezerra,Nilfácio PradoAlmeida,Paulo Roberto Carvalho deRibeiro,Ronaldo de Albuquerqueeng2008-03-27T00:00:00Zoai:scielo:S0102-86502008000100014Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2008-03-27T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Experimental rat lung tumor model with intrabronchial tumor cell implantation
title Experimental rat lung tumor model with intrabronchial tumor cell implantation
spellingShingle Experimental rat lung tumor model with intrabronchial tumor cell implantation
Gomes Neto,Antero
Lung neoplasms
Walker-256 carcinoma
rats
title_short Experimental rat lung tumor model with intrabronchial tumor cell implantation
title_full Experimental rat lung tumor model with intrabronchial tumor cell implantation
title_fullStr Experimental rat lung tumor model with intrabronchial tumor cell implantation
title_full_unstemmed Experimental rat lung tumor model with intrabronchial tumor cell implantation
title_sort Experimental rat lung tumor model with intrabronchial tumor cell implantation
author Gomes Neto,Antero
author_facet Gomes Neto,Antero
Simão,Antônio Felipe Leite
Miranda,Samuel de Paula
Mourão,Lívia Talita Cajaseiras
Bezerra,Nilfácio Prado
Almeida,Paulo Roberto Carvalho de
Ribeiro,Ronaldo de Albuquerque
author_role author
author2 Simão,Antônio Felipe Leite
Miranda,Samuel de Paula
Mourão,Lívia Talita Cajaseiras
Bezerra,Nilfácio Prado
Almeida,Paulo Roberto Carvalho de
Ribeiro,Ronaldo de Albuquerque
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes Neto,Antero
Simão,Antônio Felipe Leite
Miranda,Samuel de Paula
Mourão,Lívia Talita Cajaseiras
Bezerra,Nilfácio Prado
Almeida,Paulo Roberto Carvalho de
Ribeiro,Ronaldo de Albuquerque
dc.subject.por.fl_str_mv Lung neoplasms
Walker-256 carcinoma
rats
topic Lung neoplasms
Walker-256 carcinoma
rats
description PURPOSE: The objective of this study was to develop a rat lung tumor model for anticancer drug testing. METHODS: Sixty-two female Wistar rats weighing 208 ± 20 g were anesthetized intraperitoneally with 2.5% tribromoethanol (1 ml/100 g live weight), tracheotomized and intubated with an ultrafine catheter for inoculation with Walker's tumor cells. In the first step of the experiment, a technique was established for intrabronchial implantation of 10(5) to 5×10(5) tumor cells, and the tumor take rate was determined. The second stage consisted of determining tumor volume, correlating findings from high-resolution computed tomography (HRCT) with findings from necropsia and determining time of survival. RESULTS: The tumor take rate was 94.7% for implants with 4×10(5) tumor cells, HRCT and necropsia findings matched closely (r=0.953; p<0.0001), the median time of survival was 11 days, and surgical mortality was 4.8%. CONCLUSION: The present rat lung tumor model was shown to be feasible: the take rate was high, surgical mortality was negligible and the procedure was simple to perform and easily reproduced. HRCT was found to be a highly accurate tool for tumor diagnosis, localization and measurement and may be recommended for monitoring tumor growth in this model.
publishDate 2008
dc.date.none.fl_str_mv 2008-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000100014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000100014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502008000100014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.23 n.1 2008
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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