Nitric oxide levels in cochlear fluids of guinea pig
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500013 |
Resumo: | PURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method. |
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Acta Cirúrgica Brasileira (Online) |
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Nitric oxide levels in cochlear fluids of guinea pigNitric OxideCochleaGuinea PigsPURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2013-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500013Acta Cirúrgica Brasileira v.28 n.5 2013reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502013000500013info:eu-repo/semantics/openAccessYonamine,Fernando KaoruOnishi,Ektor TsuneoMouro,Margareth GoriHiga,Elisa Mieko SuemitsuCruz,Oswaldo Laércio Mendonçaeng2013-05-20T00:00:00Zoai:scielo:S0102-86502013000500013Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2013-05-20T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Nitric oxide levels in cochlear fluids of guinea pig |
title |
Nitric oxide levels in cochlear fluids of guinea pig |
spellingShingle |
Nitric oxide levels in cochlear fluids of guinea pig Yonamine,Fernando Kaoru Nitric Oxide Cochlea Guinea Pigs |
title_short |
Nitric oxide levels in cochlear fluids of guinea pig |
title_full |
Nitric oxide levels in cochlear fluids of guinea pig |
title_fullStr |
Nitric oxide levels in cochlear fluids of guinea pig |
title_full_unstemmed |
Nitric oxide levels in cochlear fluids of guinea pig |
title_sort |
Nitric oxide levels in cochlear fluids of guinea pig |
author |
Yonamine,Fernando Kaoru |
author_facet |
Yonamine,Fernando Kaoru Onishi,Ektor Tsuneo Mouro,Margareth Gori Higa,Elisa Mieko Suemitsu Cruz,Oswaldo Laércio Mendonça |
author_role |
author |
author2 |
Onishi,Ektor Tsuneo Mouro,Margareth Gori Higa,Elisa Mieko Suemitsu Cruz,Oswaldo Laércio Mendonça |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Yonamine,Fernando Kaoru Onishi,Ektor Tsuneo Mouro,Margareth Gori Higa,Elisa Mieko Suemitsu Cruz,Oswaldo Laércio Mendonça |
dc.subject.por.fl_str_mv |
Nitric Oxide Cochlea Guinea Pigs |
topic |
Nitric Oxide Cochlea Guinea Pigs |
description |
PURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502013000500013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.28 n.5 2013 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126441772810240 |