Nitric oxide levels in cochlear fluids of guinea pig

Detalhes bibliográficos
Autor(a) principal: Yonamine, Fernando Kaoru [UNIFESP]
Data de Publicação: 2013
Outros Autores: Onishi, Ektor Tsuneo [UNIFESP], Mouro, Margaret Gori [UNIFESP], Higa, Elisa Mieko Suemitsu [UNIFESP], Cruz, Oswaldo Laércio Mendonça [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/00130000032pp
Texto Completo: http://dx.doi.org/10.1590/S0102-86502013000500013
http://repositorio.unifesp.br/handle/11600/7742
Resumo: PURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.
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spelling Nitric oxide levels in cochlear fluids of guinea pigNitric OxideCochleaGuinea PigsPURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.Universidade Federal de São Paulo (UNIFESP) Department of Otorhinolaryngology, Head and Neck SurgeryUNIFESP Division of NephrologyUNIFESP Division of Nephrology Division of Emergency Medicine and Evidence Based Medicine. Associate ResearcherUNIFESP, Department of Otorhinolaryngology, Head and Neck SurgeryUNIFESP, Division of NephrologyUNIFESP, Division of Nephrology Division of Emergency Medicine and Evidence Based Medicine. Associate ResearcherSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Yonamine, Fernando Kaoru [UNIFESP]Onishi, Ektor Tsuneo [UNIFESP]Mouro, Margaret Gori [UNIFESP]Higa, Elisa Mieko Suemitsu [UNIFESP]Cruz, Oswaldo Laércio Mendonça [UNIFESP]2015-06-14T13:45:25Z2015-06-14T13:45:25Z2013-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion397-402application/pdfhttp://dx.doi.org/10.1590/S0102-86502013000500013Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 397-402, 2013.10.1590/S0102-86502013000500013S0102-86502013000500013.pdf0102-8650S0102-86502013000500013http://repositorio.unifesp.br/handle/11600/7742WOS:000319994200013ark:/48912/00130000032ppengActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T00:00:10Zoai:repositorio.unifesp.br/:11600/7742Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T19:53:59.658503Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Nitric oxide levels in cochlear fluids of guinea pig
title Nitric oxide levels in cochlear fluids of guinea pig
spellingShingle Nitric oxide levels in cochlear fluids of guinea pig
Yonamine, Fernando Kaoru [UNIFESP]
Nitric Oxide
Cochlea
Guinea Pigs
title_short Nitric oxide levels in cochlear fluids of guinea pig
title_full Nitric oxide levels in cochlear fluids of guinea pig
title_fullStr Nitric oxide levels in cochlear fluids of guinea pig
title_full_unstemmed Nitric oxide levels in cochlear fluids of guinea pig
title_sort Nitric oxide levels in cochlear fluids of guinea pig
author Yonamine, Fernando Kaoru [UNIFESP]
author_facet Yonamine, Fernando Kaoru [UNIFESP]
Onishi, Ektor Tsuneo [UNIFESP]
Mouro, Margaret Gori [UNIFESP]
Higa, Elisa Mieko Suemitsu [UNIFESP]
Cruz, Oswaldo Laércio Mendonça [UNIFESP]
author_role author
author2 Onishi, Ektor Tsuneo [UNIFESP]
Mouro, Margaret Gori [UNIFESP]
Higa, Elisa Mieko Suemitsu [UNIFESP]
Cruz, Oswaldo Laércio Mendonça [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Yonamine, Fernando Kaoru [UNIFESP]
Onishi, Ektor Tsuneo [UNIFESP]
Mouro, Margaret Gori [UNIFESP]
Higa, Elisa Mieko Suemitsu [UNIFESP]
Cruz, Oswaldo Laércio Mendonça [UNIFESP]
dc.subject.por.fl_str_mv Nitric Oxide
Cochlea
Guinea Pigs
topic Nitric Oxide
Cochlea
Guinea Pigs
description PURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.
publishDate 2013
dc.date.none.fl_str_mv 2013-05-01
2015-06-14T13:45:25Z
2015-06-14T13:45:25Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502013000500013
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 397-402, 2013.
10.1590/S0102-86502013000500013
S0102-86502013000500013.pdf
0102-8650
S0102-86502013000500013
http://repositorio.unifesp.br/handle/11600/7742
WOS:000319994200013
dc.identifier.dark.fl_str_mv ark:/48912/00130000032pp
url http://dx.doi.org/10.1590/S0102-86502013000500013
http://repositorio.unifesp.br/handle/11600/7742
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 397-402, 2013.
10.1590/S0102-86502013000500013
S0102-86502013000500013.pdf
0102-8650
S0102-86502013000500013
WOS:000319994200013
ark:/48912/00130000032pp
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 397-402
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1818602392584716288

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